MINFLUX nanoscopy enhanced with high-order vortex beams

Xiao-Jie Tan; Zhiwei Huang

doi:10.1038/s41377-025-01822-0

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MINFLUX nanoscopy enhanced with high-order vortex beams

Original Articles

- MINFLUX nanoscopy enhanced with high-order vortex beams
- MINFLUX nanoscopy enhanced with high-order vortex beams
- LSA 2025年14卷第7期页码：1893-1900
- Affiliations：
  
  1.Optical Bioimaging Laboratory, Department of Biomedical Engineering, College of Design and Engineering, National University of Singapore, Singapore 117576, Singapore
  2.National University of Singapore (Suzhou) Research Institute, Suzhou, Jiangsu 215123, China
  3.NUS Graduate School for Integrative Sciences and Engineering Programme (ISEP), National University of Singapore, Singapore 119077, Singapore
- Author bio：
  
  Zhiwei Huang (biehzw@nus.edu.sg)
- Funds：
- DOI：10.1038/s41377-025-01822-0
  中图分类号：
- 收稿日期：2024-09-18，
  
  修回日期：2025-02-20，
  
  录用日期：2025-03-09，
  
  网络出版日期：2025-05-06，
  
  纸质出版日期：2025-07-31
- Accepted：
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MINFLUX nanoscopy enhanced with high-order vortex beams[J]. LSA, 2025,14(7):1893-1900.

Tan, X. J. & Huang, Z. W. MINFLUX nanoscopy enhanced with high-order vortex beams. Light: Science & Applications, 14, 1893-1900 (2025).
MINFLUX nanoscopy enhanced with high-order vortex beams[J]. LSA, 2025,14(7):1893-1900. DOI： 10.1038/s41377-025-01822-0.

Tan, X. J. & Huang, Z. W. MINFLUX nanoscopy enhanced with high-order vortex beams. Light: Science & Applications, 14, 1893-1900 (2025). DOI： 10.1038/s41377-025-01822-0.

摘要

Abstract

Minimal photon fluxes (MINFLUX) nanoscopy has emerged as a transformative advancement in superresolution imaging

enabling unprecedented nanoscale observations across diverse biological scenarios. In this work

we propose

for the first time

that employing high-order vortex beams can significantly enhance the performance of MINFLUX

surpassing the limitations of the conventional MINFLUX using the first-order vortex beam. Our theoretical analysis indicates that

for standard MINFLUX

high-order vortex beams can improve the maximum localization precision by a factor corresponding to their order

which can approach a sub-nanometer scale under optimal conditions

and for raster scan MINFLUX

high-order vortex beams allow for a wider field of view while maintaining enhanced precision. These findings underscore the potential of high-order vortex beams to elevate the performance of MINFLUX

paving the way towards ultra-high resolution imaging for a broad range of applications.

关键词

Keywords

references

Hell, S. W. & Wichmann, J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Opt. Lett. 19 , 780–782 (1994)..

Hess, S. T., Girirajan, T. P. K. & Mason, M. D. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophysical J. 91 , 4258–4272 (2006)..

Rust, M. J., Bates, M. & Zhuang, X. W. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat. Methods 3 , 793–796 (2006)..

Balzarotti, F. et al. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Science 355 , 606–612 (2017)..

Eilers, Y. et al. MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution. Proc. Natl Acad. Sci. USA 115 , 6117–6122 (2018)..

Gwosch, K. C. et al. MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells. Nat. Methods 17 , 217–224 (2020)..

Masullo, L. A. et al. Pulsed interleaved MINFLUX. Nano Lett. 21 , 840–846 (2021)..

Pape, J. K. et al. Multicolor 3D MINFLUX nanoscopy of mitochondrial MICOS proteins. Proc. Natl Acad. Sci. USA 117 , 20607–20614 (2020)..

Schmidt, R. et al. MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope. Nat. Commun. 12 , 1478 (2021)..

Ostersehlt, L. M. et al. DNA-PAINT MINFLUX nanoscopy. Nat. Methods 19 , 1072–1075 (2022)..

Grabner, C. P. et al. Resolving the molecular architecture of the photoreceptor active zone with 3D-MINFLUX. Sci. Adv. 8 , eabl7560 (2022)..

Wirth, J. O. et al. MINFLUX dissects the unimpeded walking of kinesin-1. Science 379 , 1004–1010 (2023)..

Deguchi, T. et al. Direct observation of motor protein stepping in living cells using MINFLUX. Science 379 , 1010–1015 (2023)..

Remmel, M. et al. Accelerated MINFLUX Nanoscopy, through spontaneously fast‐blinking fluorophores. Small 19 , 2206026 (2023)..

Zähringer, J. et al. Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy. Light Sci. Appl. 12 , 70 (2023)..

Cole, F. et al. Super-resolved FRET and co-tracking in pMINFLUX. Nat. Photonics 18 , 478–484 (2024)..

Rickert, J. D. et al. 4Pi MINFLUX arrangement maximizes spatio-temporal localization precision of fluorescence emitter. Proc. Natl Acad. Sci. USA 121 , e2318870121 (2024)..

Masullo, L. A. et al. An alternative to MINFLUX that enables nanometer resolution in a confocal microscope. Light Sci. Appl. 11 , 199 (2022)..

Zhao, K. et al. Two-photon MINFLUX with doubled localization precision. eLight 2 , 5 (2022)..

Denk, W., Strickler, J. H. & Webb, W. W. Two-photon laser scanning fluorescence microscopy. Science 248 , 73–76 (1990)..

Lin, B. C. et al. Theoretical study of the properties of multiphoton MINFLUX and RASTMIN super-resolution imaging. Appl. Phys. Lett. 123 , 223701 (2023)..

Hoover, E. E. & Squier, J. A. Advances in multiphoton microscopy technology. Nat. Photonics 7 , 93–101 (2013)..

Allen, L. et al. Orbital angular momentum of light and the transformation of Laguerre-Gaussian laser modes. Phys. Rev. A 45 , 8185–8189 (1992)..

Yao, A. M. & Padgett, M. J. Orbital angular momentum: origins, behavior and applications. Adv. Opt. Photonics 3 , 161–204 (2011)..

Shen, Y. J. et al. Optical vortices 30 years on: OAM manipulation from topological charge to multiple singularities. Light Sci. Appl. 8 , 90 (2019)..

Xu, X. Z., Jia, S. & Xi, P. Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes. LightSci. Appl. 11 , 293 (2022)..

Van Trees, H. L. Detection, Estimation, and Modulation Theory, Part Ⅰ: Detection, Estimation, and Linear Modulation Theory. (John Wiley&Sons, 2004).

Gill, R. D. & Levit, B. Y. Applications of the van Trees inequality: a Bayesian Cramér-Rao bound. Bernoulli 1 , 59–79 (1995)..

Masullo, L. A., Lopez, L. F. & Stefani, F. D. A common framework for single-molecule localization using sequential structured illumination. Biophysical Rep. 2 , 100036 (2022)..

Wang, W. B. et al. Deep transmission of Laguerre–Gaussian vortex beams through turbid scattering media. Opt. Lett. 41 , 2069–2072 (2016)..

Mamani, S. et al. Transmission of classically entangled beams through mouse brain tissue. J. Biophotonics 11 , e201800096 (2018)..

He, C. Y. et al. Effects of optical aberrations on localization of MINFLUX super-resolution microscopy. Opt. Express 30 , 46849–46860 (2022)..

Rubano, A. et al. Q-plate technology: a progress review [Invited ] . J. Optical Society America B 36 , D70–D87 (2019)..

Forbes, A., Dudley, A. & McLaren, M. Creation and detection of optical modes with spatial light modulators. Adv. Opt. Photonics 8 , 200–227 (2016)..

Ahmed, H. et al. Optical metasurfaces for generating and manipulating optical vortex beams. Nanophotonics 11 , 941–956 (2022)..

Ramachandran, S. & Kristensen, P. Optical vortices in fiber. Nanophotonics 2 , 455–474 (2013)..

Forbes, A., Mkhumbuza, L. & Feng, L. Orbital angular momentum lasers. Nat. Rev. Phys. 6 , 352–364 (2024)..

Ruffato, G., Massari, M. & Romanato, F. Generation of high-order Laguerre–Gaussian modes by means of spiral phase plates. Opt. Lett. 39 , 5094–5097 (2014)..

Deguchi, T. & Ries, J. Simple and robust 3D MINFLUX excitation with a variable phase plate. Light Sci. Appl. 13 , 134 (2024)..

Astratov, V. N. et al. Roadmap on label‐free super‐resolution imaging. Laser Photonics Rev. 17 , 2200029 (2023)..

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