Array detection enables large localization range for simple and robust MINFLUX

Eli Slenders; Sanket Patil; Marcus Oliver Held; Alessandro Zunino; Giuseppe Vicidomini

doi:10.1038/s41377-025-01883-1

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Array detection enables large localization range for simple and robust MINFLUX

Original Articles

- Array detection enables large localization range for simple and robust MINFLUX
- Array detection enables large localization range for simple and robust MINFLUX
- LSA 2025年14卷第9期页码：2476-2488
- Affiliations：
  
  1.Molecular Microscopy and Spectroscopy, Istituto Italiano di Tecnologia, Genoa, Italy
  2.Department of Informatics, Bioengineering, Robotics and Systems Engineering (DIBRIS), University of Genoa, Genoa, Italy
- Author bio：
  
  Giuseppe Vicidomini (giuseppe.vicidomini@iit.it)
- Funds：
- DOI：10.1038/s41377-025-01883-1
  中图分类号：
- 收稿日期：2025-01-27，
  
  修回日期：2025-04-15，
  
  录用日期：2025-05-01，
  
  网络出版日期：2025-07-03，
  
  纸质出版日期：2025-09-30
- Accepted：
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Array detection enables large localization range for simple and robust MINFLUX[J]. LSA, 2025,14(9):2476-2488.

Slenders, E. et al. Array detection enables large localization range for simple and robust MINFLUX. Light: Science & Applications, 14, 2476-2488 (2025).
Array detection enables large localization range for simple and robust MINFLUX[J]. LSA, 2025,14(9):2476-2488. DOI： 10.1038/s41377-025-01883-1.

Slenders, E. et al. Array detection enables large localization range for simple and robust MINFLUX. Light: Science & Applications, 14, 2476-2488 (2025). DOI： 10.1038/s41377-025-01883-1.

摘要

Abstract

The MINFLUX concept significantly improves the localization properties of single-molecule localization microscopy (SMLM) by overcoming the limit imposed by the fluorophore's photon counts. Typical MINFLUX microscopes localize the target molecule by scanning a zero-intensity focus around the molecule in a circular trajectory

with smaller trajectory diameters yielding better localization uncertainties for a given number of photons. Since this approach requires the molecule to be within the scanned trajectory

MINFLUX typically relies on an iterative scheme with decreasing trajectory diameters. This iterative approach is prone to misplacements of the trajectory and increases the system's complexity. In this work

we introduce ISM-FLUX

a novel implementation of MINFLUX using image-scanning microscopy (ISM) with a single-photon avalanche diode array detector. ISM-FLUX provides a precise MINFLUX localization within the trajectory while maintaining a conventional photon-limited uncertainty outside it. The robustness of ISM-FLUX localization results in a larger localization range and greatly simplifies the architecture

which may facilitate broader adoption of MINFLUX.

关键词

Keywords

references

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