Ultrahigh-throughput single-pixel complex-field microscopy with frequency-comb acousto-optic coherent encoding (FACE)

Daixuan Wu; Yuecheng Shen; Zhongzheng Zhu; Tijian Li; Jiawei Luo; Zhengyang Wang; Jiaming Liang; Zhiling Zhang; Yunhua Yao; Dalong Qi; Lianzhong Deng; Zhenrong Sun; Meng Liu; Zhi-Chao Luo; Shian Zhang

doi:10.1038/s41377-025-01931-w

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Ultrahigh-throughput single-pixel complex-field microscopy with frequency-comb acousto-optic coherent encoding (FACE)

Original Articles

- Ultrahigh-throughput single-pixel complex-field microscopy with frequency-comb acousto-optic coherent encoding (FACE)
- Ultrahigh-throughput single-pixel complex-field microscopy with frequency-comb acousto-optic coherent encoding (FACE)
- LSA 2025年14卷第9期页码：2752-2763
- Affiliations：
  
  1.Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, Guangdong 510006, China
  2.State Key Laboratory of Precision Spectroscopy, School of Physics and Electronic Science, East China Normal University, Shanghai 200241, China
  3.School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou 510006, China
  4.MOE Key Laboratory of Laser Life Science, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, China
  5.Joint Research Center of Light Manipulation Science and Photonic Integrated Chip of East China Normal University and Shandong Normal University, East China Normal University, Shanghai 200241, China
  6.Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan 030006, China
- Author bio：
  
  Yuecheng Shen (ycshen@lps.ecnu.edu.cn)
  Zhi-Chao Luo (zcluo@scnu.edu.cn)
  Shian Zhang (sazhang@phy.ecnu.edu.cn)
- Funds：
- DOI：10.1038/s41377-025-01931-w
  中图分类号：
- 收稿日期：2024-12-02，
  
  修回日期：2025-05-22，
  
  录用日期：2025-06-21，
  
  网络出版日期：2025-08-11，
  
  纸质出版日期：2025-09-30
- Accepted：
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Ultrahigh-throughput single-pixel complex-field microscopy with frequency-comb acousto-optic coherent encoding (FACE)[J]. LSA, 2025,14(9):2752-2763.

Wu, D X. et al. Ultrahigh-throughput single-pixel complex-field microscopy with frequency-comb acousto-optic coherent encoding (FACE). Light: Science & Applications, 14, 2752-2763 (2025).
Ultrahigh-throughput single-pixel complex-field microscopy with frequency-comb acousto-optic coherent encoding (FACE)[J]. LSA, 2025,14(9):2752-2763. DOI： 10.1038/s41377-025-01931-w.

Wu, D X. et al. Ultrahigh-throughput single-pixel complex-field microscopy with frequency-comb acousto-optic coherent encoding (FACE). Light: Science & Applications, 14, 2752-2763 (2025). DOI： 10.1038/s41377-025-01931-w.

摘要

Abstract

Single-pixel imaging (SPI) is a promising technology for optical imaging beyond the visible spectrum

where commercial cameras are expen

sive or unavailable. However

limitations such as slow pattern projection rates and time-consuming reconstruction algorithms hinder its throughput for real-time imaging. Consequently

conventional SPI is inadequate for high-speed

high-resolution tasks. To address these challenges

we developed an ultrahigh-throughput single-pixel complex-field microscopy (SPCM) system utilizing frequency-comb acousto-optic coherent encoding (FACE). This system enables real-time complex-field monitoring in the non-visible domain. Operating at 1030 nm

our system achieves a record-high space-bandwidth-time product (SBP-T) of 1.3 × 10

surpassing previous SPCM (~10

)

SPI (~10

)

and even certain types of commercial near-infrared cameras (~10

). It supports real-time streaming at 1000 Hz with a frame size of 80 × 81 pixels and a lateral resolution of 3.76 μm across an approximately 300 μm field of view. We validated the system by imaging dynamic transparent scenes

including microfluidics

live microorganisms

chemical reactions

as well as imaging through scattering media. This advancement offers a superior solution for high-speed

high-resolution complex-field imaging beyond the visible spectrum

significantly enhancing SPI performance across various applications.

关键词

Keywords

references

Stantchev, R. I. et al. Noninvasive, near-field terahertz imaging of hidden objects using a single-pixel detector. Sci. Adv. 2 , e1600190 (2016)..

Radwell, N. et al. Single-pixel infrared and visible microscope. Optica 1 , 285–289 (2014)..

Manley, M. Near-infrared spectroscopy and hyperspectral imaging: non-destructive analysis of biological materials. Chem. Soc. Rev. 43 , 8200–8214 (2014)..

Shi, L. Y. et al. Transmission in near-infrared optical windows for deep brain imaging. J. Biophotonics 9 , 38–43 (2016)..

Kenry, Duan, Y. K. & Liu, B. Recent advances of optical imaging in the second near-infrared window. Adv. Mater. 30 , 1802394 (2018)..

Zhang, Z. B., Ma, X. & Zhong, J. G. Sing le-pixel imaging by means of Fourier spectrum acquisition. Nat. Commun. 6 , 6225 (2015)..

Edgar, M. P. et al. Simultaneous real-time visible and infrared video with single-pixel detectors. Sci. Rep. 5 , 10669 (2015)..

Sun, M. J. et al. Single-pixel three-dimensional imaging with time-based depth resolution. Nat. Commun. 7 , 12010 (2016)..

Altmann, Y. et al. Quantum-inspired computational imaging. Science 361 , eaat2298 (2018)..

Meng, H. Y. et al. Quantum dot-enabled infrared hyperspectral imaging with single-pixel detection. Light 13 , 121 (2024)..

Sun, B. et al. 3D computational imaging with single-pixel detectors. Science 340 , 844–847 (2013)..

Kirmani, A. et al. First-photon imaging. Science 343 , 58–61 (2014)..

Huynh, N. et al. Single-pixel optical camera for video rate ultrasonic imaging. Optica 3 , 26–29 (2016)..

Phillips, D. B. et al. Adaptive foveated single-pixel imaging with dynamic supersampling. Sci. Adv. 3 , e1601782 (2017)..

Soldevila, F. et al. Phase imaging by spatial wavefront sampling. Optica 5 , 164–174 (2018)..

Xu, Z. H. et al. 1000 fps computational ghost imaging using LED-based structured illumination. Opt. Express 26 , 2427–2434 (2018)..

Zhao, J. P. et al. Compressive ultrafast pulse measurement via time-domain single-pixel imaging. Optica 8 , 1176–1185 (2021)..

Liu, Y. F. et al. Optical single-pixel volumetric imaging by three-dimensional light-field illumination. Proc. Natl Acad. Sci. USA 120 , e2304755120 (2023)..

Bian, L. H. et al. High-resolution single-photon imaging with physics-informed deep learning. Nat. Commun. 14 , 5902 (2023)..

Wang, Y. Q. et al. Mid-infrared single-pixel imaging at the single-photon level. Nat. Commun. 14 , 1073 (2023)..

Zeng, B. B. et al. Hybrid graphene metasurfaces for high-speed mid-infrared light modulation and single-pixel imaging. Light 7 , 51 (2018)..

Stantchev, R. I. et al. Real-time terahertz imaging with a single-pixel detector. Nat. Commun. 11 , 2535 (2020)..

Li, J. X. et al. Rapid sensing of hidden objects and defects using a single-pixel diffractive terahertz sensor. Nat. Commun. 14 , 6791 (2023)..

Li, W. L. et al. Dual-color terahertz spatial light modulator for single-pixel imaging. Light 11 , 191 (2022)..

Liang, J. Y. et al. Spatially Fourier-encoded photoacoustic microscopy using a digital micromirror device. Opt. Lett. 39 , 430–433 (2014)..

Huynh, N. et al. Single-pixel camera photoacoustic tomography. J. Biomed. Opt. 24 , 121907 (2019)..

Clemente, P. et al. Compressive holography with a single-pixel detector. Opt. Lett. 38 , 2524–2527 (2013)..

Martínez-León, L. et al. Single-pixel digital holography with phase-encoded illumination. Opt. Express 25 , 4975–4984 (2017)..

González, H. et al. High sampling rate single-pixel digital holography system employing a DMD and phase-encoded patterns. Opt. Express 26 , 20342–20350 (2018)..

Baek, Y. & Park, Y. Intensity-based holographic imaging via space-domain Kramers–Kronig relations. Nat. Photonics 15 , 354–360 (2021)..

Tian, L. et al. Computational illumination for high-speed in vitro Fourier ptychographic microscopy. Optica 2 , 904–911 (2015)..

Liu, S. Y., Mulligan, J. A. & Adie, S. G. Volumetric optical coherence microscopy with a high space-bandwidth- time product enabled by hybrid adaptive optics. Biomed. Opt. Express 9 , 3137–3152 (2018)..

Higham, C. F. et al. Deep learning for real-time single-pixel video. Sci. Rep. 8 , 2369 (2018)..

Edgar, M. P., Gibson, G. M. & Padgett, M. J. Principles and prospects for single-pixel imaging. Nat. Photonics 13 , 13–20 (2019)..

Stellinga, D. et al. Time-of-flight 3D imaging through multimode optical fibers. Science 374 , 1395–1399 (2021)..

Jiang, W. J. et al. 2, 000, 000 fps 2D and 3D imaging of periodic or reproducible scenes with single-pixel detectors. Photonics Res. 10 , 2157–2164 (2022)..

Wu, D. X. et al. Imaging biological tissue with high-throughput single-pixel compressive holography. Nat. Commun. 12 , 4712 (2021)..

Hahamovich, E. et al. Si ngle pixel imaging at megahertz switching rates via cyclic Hadamard masks. Nat. Commun. 12 , 4516 (2021)..

Kilcullen, P., Ozaki, T. & Liang, J. Y. Compressed ultrahigh-speed single-pixel imaging by swept aggregate patterns. Nat. Commun. 13 , 7879 (2022)..

Goda, K., Tsia, K. K. & Jalali, B. Serial time-encoded amplified imaging for real-time observation of fast dynamic phenomena. Nature 458 , 1145–1149 (2009)..

Diebold, E. D. et al. Digitally synthesized beat frequency multiplexing for sub-millisecond fluorescence microscopy. Nat. Photonics 7 , 806–810 (2013)..

Wu, J. L. et al. Ultrafast laser-scanning time-stretch imaging at visible wavelengths. Light 6 , e16196 (2017)..

Teng, J. J. et al. Time-encoded single-pixel 3D imaging. APL Photonics 5 , 020801 (2020)..

Schraivogel, D. et al. High-speed fluorescence image–enabled cell sorting. Science 375 , 315–320 (2022)..

Liu, J. D. et al. Swept coded aperture real-time femtophotography. Nat. Commun. 15 , 1589 (2024)..

Lei, C. et al. High-throughput imaging flow cytometry by optofluidic time-stretch microscopy. Nat. Protoc. 13 , 1603–1631 (2018)..

Han, Y. Y. et al. Cameraless high-throughput three-dimensional imaging flow cytometry. Optica 6 , 1297–1304 (2019)..

Zhang, Z. M. et al. A high-throughput technique to map cell images to cell positions using a 3D imaging flow cytometer. Proc. Natl Acad. Sci. USA 119 , e2118068119 (2022)..

Chan, A. C. S. et al. Arbitrary two-dimensional spectrally encoded pattern generation—a new strategy for high-speed patterned illumination imaging. Optica 2 , 1037–1044 (2015)..

Feldkhun, D. et al. Focusing and scanning through scattering media in microseconds. Optica 6 , 72–75 (2019)..

Wei, X. M. et al. Real-time frequency-encoded spatiotemporal focusing through scattering media using a programmable 2D ultrafine optical frequency comb. Sci. Adv. 6 , eaay1192 (2020)..

Tajahuerce, E. et al. Image transmission through dynamic scattering media by single-pixel photodetection. Opt. Express 22 , 16945–16955 (2014)..

Durán, V. et al. Compressive imaging in scattering media. Opt. Express 23 , 14424–14433 (2015)..

Lee, W. B. et al. COMPU-EYE: a high resolution computational compound eye. Opt. Express 24 , 2013–2026 (2016)..

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