Laser-emission vibrational microscopy of microdroplet arrays for high-throughput screening of hyperlipidemia

Zhonghao Li; Zhihan Cai; Yuhan Wang; Yuliang Liu; Guifeng Li; Xi Yang; Ming Deng; Yu-Cheng Chen; Jichun Yang; Yang Luo; Chaoyang Gong; Tao Zhu

doi:10.1038/s41377-025-02015-5

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Laser-emission vibrational microscopy of microdroplet arrays for high-throughput screening of hyperlipidemia

Original Articles

- Laser-emission vibrational microscopy of microdroplet arrays for high-throughput screening of hyperlipidemia
- Laser-emission vibrational microscopy of microdroplet arrays for high-throughput screening of hyperlipidemia
- Light: Science & Applications 2025年14卷第12期页码：3925-3934
- Affiliations：
  
  1.Key Laboratory of Optoelectronic Technology and Systems (Ministry of Education of China), School of Optoelectronic Engineering, Chongqing University, Chongqing 400044, China
  2.Department of Laboratory Medicine, Chongqing Center for Clinical Laboratory, Chongqing Academy of Medical Sciences, Chongqing General Hospital, School of Medicine, Chongqing University, Chongqing 401147, China
  3.Key Laboratory of Optical Fiber Sensing and Communications (Ministry of Education of China), School of Information and Communication Engineering, University of Electronic Science and Technology of China, Chengdu, Sichuan 611731, China
  4.School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore
  5.Lee Kong Chian School of Medicine, Singapore 636921, Singapore
- Author bio：
  
  Yu-Cheng Chen (yucchen@ntu.edu.sg)
  Jichun Yang (yangjichun@cqu.edu.cn)
  Yang Luo (Luoy@cqu.edu.cn)
  Chaoyang Gong (cygong@cqu.edu.cn)
- Funds：
- DOI：10.1038/s41377-025-02015-5
  中图分类号：
- 收稿：2025-04-09，
  
  修回：2025-07-31，
  
  录用：2025-08-18，
  
  网络出版：2025-09-17，
  
  纸质出版：2025-12-31
- Accepted：
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Zhonghao Li, Zhihan Cai, Yuhan Wang, 等. Laser-emission vibrational microscopy of microdroplet arrays for high-throughput screening of hyperlipidemia[J]. Light: Science & Applications, 2025,14(12):3925-3934.

Li, Z. H. et al. Laser-emission vibrational microscopy of microdroplet arrays for high-throughput screening of hyperlipidemia. Light: Science & Applications, 14, 3925-3934 (2025).
Zhonghao Li, Zhihan Cai, Yuhan Wang, 等. Laser-emission vibrational microscopy of microdroplet arrays for high-throughput screening of hyperlipidemia[J]. Light: Science & Applications, 2025,14(12):3925-3934. DOI： 10.1038/s41377-025-02015-5.

Li, Z. H. et al. Laser-emission vibrational microscopy of microdroplet arrays for high-throughput screening of hyperlipidemia. Light: Science & Applications, 14, 3925-3934 (2025). DOI： 10.1038/s41377-025-02015-5.

摘要

Abstract

The mechanical properties of biological fluids serve as early indicators of disease

offering valuable insights into complex physiological and pathological processes. However

the existing technologies struggle to achieve high-throughput measurement

limiting their widespread applications in disease diagnosis. Here

we propose laser-emission vibrational microscopy of microdroplets for high-throughput measurement of the intrinsic mechanical properties of fluids. The microdroplet array supporting high Q-factor (10

) whispering gallery modes (WGM) lasing was massively fabricated on a superhydrophobic surface with inkjet printing. Ultrasound was employed to actuate the mechanical vibrations of the microdroplets

and the vibration amplitude was quantified using time-resolved laser spectra. We found that the stimulus-response of the laser emission is strongly dependent on the liquid viscosity. Fast mapping of the microdroplets' viscosities was achieved by stage scanning. High-throughput screening of hyperlipidemia disease was also demonstrated by performing over 2000 measurements within 25 min. Thanks to the small volume of the microdroplets

a single drop of blood can support over seven million measurements. The high-throughput ability and small sample consumption make it a promising tool for clinical diagnoses based on mechanical properties.

关键词

Keywords

references

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