Dual-frequency fiber-array photoacoustic computed tomography for high-resolution deep brain imaging

Zitao Chen; Yuhan Wu; Hexiang Xu; Lanling Liang; Jun Ma; Yi Zhang; Bai-Ou Guan

doi:10.1038/s41377-026-02324-3

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Dual-frequency fiber-array photoacoustic computed tomography for high-resolution deep brain imaging

Original Articles

- Dual-frequency fiber-array photoacoustic computed tomography for high-resolution deep brain imaging
- Dual-frequency fiber-array photoacoustic computed tomography for high-resolution deep brain imaging
- Light: Science & Applications 2026年15卷第8期页码：2818-2832
- Affiliations：
  
  1.Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Institute of Photonics Technology, Jinan University, Guangzhou, China
  2.College of Physics & Optoelectronic Engineering, Jinan University, Guangzhou, China
  3.College of Life Science and Technology, Jinan University, Guangzhou, China
- Author bio：
  
  Jun Ma (jun.ma@jnu.edu.cn)
  Yi Zhang (zhangyi_0424hot@163.com)
  Bai-Ou Guan (tguanbo@jnu.edu.cn)
- Funds：
- DOI：10.1038/s41377-026-02324-3
  中图分类号：
- 收稿：2025-08-02，
  
  修回：2026-04-19，
  
  录用：2026-04-20，
  
  网络首发：2026-06-01，
  
  纸质出版：2026-08-31
- Accepted：
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Zitao Chen, Yuhan Wu, Hexiang Xu, 等. Dual-frequency fiber-array photoacoustic computed tomography for high-resolution deep brain imaging[J]. Light: Science & Applications, 2026,15(8):2818-2832.

Chen, Z. T. et al. Dual-frequency fiber-array photoacoustic computed tomography for high-resolution deep brain imaging. Light: Science & Applications, 15, 2818-2832 (2026).
Zitao Chen, Yuhan Wu, Hexiang Xu, 等. Dual-frequency fiber-array photoacoustic computed tomography for high-resolution deep brain imaging[J]. Light: Science & Applications, 2026,15(8):2818-2832. DOI： 10.1038/s41377-026-02324-3.

Chen, Z. T. et al. Dual-frequency fiber-array photoacoustic computed tomography for high-resolution deep brain imaging. Light: Science & Applications, 15, 2818-2832 (2026). DOI： 10.1038/s41377-026-02324-3.

摘要

Abstract

Photoacoustic tomography as an optical-ultrasound hybrid imaging modality provides rich optical contrast over the extended penetration depth of biological tissues

enabling multiscale multicontrast structural and functional imaging. However

inherent limitations in the state-of-the-art piezoelectric transducer arrays of the photoacoustic tomography

including size-dependent sensitivity

narrow bandwidth

and high material rigidity

compromise the resolution

penetration depth

and functional assessment precision. Here

an arc-shaped fiber ultrasound transducer array with a sheet-like ultrasound focus is demonstrated for photoacoustic computed tomography. At the ultrasound focus

a low detection limit of ~ 5.2 Pa and a dual-frequency response spanning several octaves are achieved. Whole mouse brain imaging with a depth up to ~ 1.2 cm and a spatial resolution of ~ 70 μm in the cerebral cortex region is showcased. The blood oxygen saturation within the entire mouse brain and the brain tumors is visualized

and the assessment precision is improved by leveraging the dual-frequency response of the transducer array. The centimeter-scale imaging depth

fine resolution of the cerebral vessels

and improved precision in the blood oxygenation evaluation make the fiber-array photoacoustic tomography a competitive candidate to the sought-after magnetic resonance imaging and ultrasound localization microscopy for brain functionality study and disease diagnosis.

关键词

Keywords

references

Weissleder, R. & Nahrendorf, M. Advancing biomedical imaging. Proc. Natl. Acad. Sci. USA 112 , 14424–14428 (2015)..

Bischof, J. et al. Multimodal bioimaging across disciplines and scales: challenges, opportunities and breaking down barriers. npj. Imaging 2 , 5 (2024)..

Wallyn, J. et al. Biomedical imaging: principles, technologies, clinical aspects, contrast agents, limitations and future trends in nanomedicines. Pharm. Res. 36 , 78 (2019)..

Errico, C. et al. Ultrafast ultrasound localization microscopy for deep super-resolution vascular imaging. Nature 527 , 499–502 (2015)..

Renaudin, N. et al. Functional ultrasound localizati on microscopy reveals brain-wide neurovascular activity on a microscopic scale. Nat. Methods 19 , 1004–1012 (2022)..

Demené, C. et al. Transcranial ultrafast ultrasound localization microscopy of brain vasculature in patients. Nat. Biomed. Eng. 5 , 219–228 (2021)..

Lin, B. Z. et al. Combined nanodrops imaging and ultrasound localization microscopy for detecting intracerebral hemorrhage. Ultrasound Med. Biol. 51 , 707–714 (2025)..

Wang, L. V. & Hu, S. Photoacoustic tomography: in vivo imaging from organelles to organs. Science 335 , 1458–1462 (2012)..

Wang, L. V. & Yao, J. J. A practical guide to photoacoustic tomography in the life sciences. Nat. Methods 13 , 627–638 (2016)..

Steinberg, I. et al. Photoacoustic clinical imaging. Photoacoustics 14 , 77–98 (2019)..

Ntziachristos, V. Addressing unmet clinical need with optoacoustic imaging. Nat. Rev. Bioeng. 3 , 182–184 (2024)..

Knieling, F., Lee, S. & Ntziachristos, V. A primer on current status and future opportunities of clinical optoacoustic imaging. npj Imaging 3 , 4 (2025)..

Lin, L. & Wang, L. V. The emerging role of photoacoustic imaging in clinical oncology. Nat. Rev. Clin. Oncol. 19 , 365–384 (2022)..

Park, J. et al. Clinical translation of photoacoustic imaging. Nat. Rev. Bioeng. 3 , 193–212 (2024)..

Aguirre, J. et al. Precision assessment of label-free psoriasis biomarkers with ultra-broadband optoacoustic mesoscopy. Nat. Biomed. Eng. 1 , 0068 (2017)..

Kalva, S. K. et al. Spiral volumetric optoacoustic tomography for imaging whole-body biodynamics in small animals. Nat. Protoc. 18 , 2124–2142 (2023)..

Choi, S. et al. Deep learning enhances multiparametric dynamic volumetric photoacoustic computed tomography in vivo (DL-PACT). Adv. Sci. 10 , 2202089 (2023)..

Lin, L. et al. High-speed three-dimensional photoacoustic computed tomography for preclinical research and clinical translation. Nat. Commun. 12 , 882 (2021)..

Ozcan, B. B. et al. Current status of optoacoustic breast imaging and future trends in clinical application: is it ready for prime time?. Eur. Radiol. 34 , 6092–6107 (2024)..

Ku, G. et al. Multiple-bandwidth photoacoustic tomography. Phys. Med. Biol. 49 , 1329–1338 (2004)..

Chekkoury, A., Gateau, J.&Ntziachristos, V. Multiple bandwidth volumetric optoacoustic tomography using conventional ultrasound linear arrays. Proceedings of SPIE 8800, Opto-Acoustic Methods and Applications. Munich, Germany: SPIE, 2013, 880003.

Zheng, Q. C. et al. Thin ceramic PZT dual- and multi-frequency pMUT arrays for photoacoustic imaging. Microsyst. Nanoeng. 8 , 122 (2022)..

Wang, H. R. et al. Development of dual-frequency PMUT arrays based on thin ceramic PZT for endoscopic photoacoustic imaging. J. Microelectromech. Syst. 30 , 770–782 (2021)..

Cai, J. X. et al. Beyond fundamental resonance mode: high-order multi-band ALN PMUT for in vivo photoacoustic imaging. Microsyst. Nanoeng. 8 , 116 (2022)..

Park, J. S. et al. Dual-frequency piezoelectric micromachined ultrasound transducer based on polarization switching in ferroelectric thin films. Microsyst. Nanoeng. 9 , 122 (2023)..

Chee, R. K. W. et al. Multifrequency interlaced CMUTs for photoacoustic imaging. IEEE Trans. Ultrason. Ferroelectr. Freq. Control 64 , 391–401 (2017)..

Subochev, P. V. et al. Ultrawideband high density polymer-based spherical array for real-time functional optoacoustic micro-angiography. Light Sci. Appl. 14 , 239 (2025)..

Shnaiderman, R. et al. A submicrometre silicon-on-insulator resonator for ultrasound detection. Nature 585 , 372–378 (2020)..

Westerveld, W. J. et al. Sensitive, small, broadband and scalable optomechanical ultrasound sensor in silicon photonics. Nat. Photonics 15 , 341–345 (2021)..

Hazan, Y. et al. Silicon-photonics acoustic detector for optoacoustic micro-tomography. Nat. Commun. 13 , 1488 (2022)..

Pan, J. S. et al. Parallel interrogation of the chalcogenide-based micro-ring sensor array for photoacoustic tomography. Nat. Commun. 14 , 3250 (2023)..

Shnaiderman, R. et al. Fiber interferometer for hybrid optical and optoacoustic intravital microscopy. Optica 4 , 1180–1187 (2017)..

Azhari, H. Basics of Biomedical Ultrasound for Engineers. (Hoboken: John Wiley&Sons, 2010).

Hatami, M. et al. Noninvasive tracking of embryonic cardiac dynamics and development with volumetric optoacoustic spectroscopy. Adv. Sci. 11 , 2400089 (2024)..

Liu, S. R. et al. Validation of photoacoustic/ultrasound dual imaging in evaluating blood oxygen saturation. Biomed. Opt. Express 13 , 5551–5570 (2022)..

Seeger, M. et al. Pushing the boundaries of optoacoustic microscopy by total impulse response characterization. Nat. Commun. 11 , 2910 (2020)..

Rosenthal, A., Razansky, D. & Ntziachristos, V. High-sensitivity compact ultrasonic detector based on a pi-phase-shifted fiber Bragg grating. Opt. Lett. 36 , 1833–1835 (2011)..

Rosenthal, A., Razansky, D. & Ntziachristos, V. Wideband optical sensing using pulse interferometry. Opt. Express 20 , 19016–19029 (2012)..

Rosenthal, A. et al. Sensitive interferometric detection of ultrasound for minimally invasive clinical imaging applications. Laser Photonics Rev. 8 , 450–457 (2014)..

Wissmeyer, G. et al. All-optical optoacoustic microscope based on wideband pulse interferometry. Opt. Lett. 41 , 1953–1956 (2016)..

Rosenthal, A. et al. Embedded ultrasound sensor in a silicon-on-insulator photonic platform. Appl. Phys. Lett. 104 , 021116 (2014)..

La, T. A. et al. Bragg grating etalon-based optical fiber for ultrasound and optoacoustic detection. Nat. Commun. 15 , 7521 (2024)..

Shnaiderman, R. et al. Silicon-photonics point sensor for high-resolution optoacoustic imaging. Adv. Opt. Mater. 9 , 2100256 (2021)..

Chen, N. B. et al. Simultaneous head-mounte d imaging of neural and hemodynamic activities at high spatiotemporal resolution in freely behaving mice. Sci. Adv. 11 , eadu1153 (2025)..

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