Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM)

Yue Zhuo; Ji Sun Choi; Thibault Marin; Hojeong Yu; Brendan A. Harley; Brian T. Cunningham

doi:10.1038/s41377-018-0001-5

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Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM)

Original Articles

- Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM)
- Light: Science & Applications Vol. 7, Issue 4, Pages: 104-118(2018)
- Affiliations：
  
  1.Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
  2.Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
  3.Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
  4.Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
  5.Atkins Building, University of Illinois Research Park, 1800 South Oak Street, Champaign, IL 61820, USA
  6.Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
- Author bio：
  
  Brian T. Cunningham (bcunning@illinois.edu)
- Funds：
- DOI：10.1038/s41377-018-0001-5
  CLC：
- Published：2018，
  
  Published Online：30 May 2018，
  
  Received：11 May 2017，
  
  Revised：13 February 2018，
  
  Accepted：14 February 2018
- Accepted：
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Zhuo, Y. et al. Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM). Light: Science & Applications, 4, 104-118 (2018).
DOI：

Zhuo, Y. et al. Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM). Light: Science & Applications, 4, 104-118 (2018). DOI： 10.1038/s41377-018-0001-5.

摘要

Abstract

Focal adhesions are critical cell membrane components that regulate adhesion and migration and have cluster dimensions that correlate closely with adhesion engagement and migration speed. We utilized a label-free approach for dynamic

long-term

quantitative imaging of cell–surface interactions called photonic resonator outcoupler microscopy (PROM) in which membrane-associated protein aggregates outcoupled photons from the resonant evanescent field of a photonic crystal biosensor

resulting in a highly localized reduction of the reflected light intensity. By mapping the changes in the resonant reflected peak intensity from the biosensor surface

we demonstrate the ability of PROM to detect focal adhesion dimensions. Similar spatial distributions can be observed between PROM images and fluorescence-labeled images of focal adhesion areas in dental epithelial stem cells. In particular

we demonstrate that cell–surface contacts and focal adhesion formation can be imaged by two orthogonal label-free modalities in PROM simultaneously

providing a general-purpose tool for kinetic

high axial-resolution monitoring of cell interactions with basement membranes.

关键词

Keywords

references

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