Plasmonic bacteria on a nanoporous mirror via hydrodynamic trapping for rapid identification of waterborne pathogens

Keumrai Whang; Jong-Hwan Lee; Yonghee Shin; Wooju Lee; Young Wan Kim; Dongchoul Kim; Luke P. Lee; Taewook Kang

doi:10.1038/s41377-018-0071-4

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Plasmonic bacteria on a nanoporous mirror via hydrodynamic trapping for rapid identification of waterborne pathogens

Original Articles

- Plasmonic bacteria on a nanoporous mirror via hydrodynamic trapping for rapid identification of waterborne pathogens
- Light: Science & Applications Vol. 7, Issue 6, Pages: 716-724(2018)
- Affiliations：
  
  1.Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 04107, Korea
  2.Berkeley Sensor and Actuator Center, Departments of Bioengineering, Electrical Engineering and Computer Science, Biophysics Graduate Program, University of California, Berkeley, Berkeley, CA, 94720, USA
  3.Department of Mechanical Engineering, Sogang University, Seoul, 04107, Korea
- Author bio：
  
  Dongchoul Kim (dckim@sogang.ac.kr)
  Luke P. Lee (lplee@berkeley.edu)
  Taewook Kang (twkang@sogang.ac.kr)
- Funds：
- DOI：10.1038/s41377-018-0071-4
  CLC：
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Whang, K. et al. Plasmonic bacteria on a nanoporous mirror via hydrodynamic trapping for rapid identification of waterborne pathogens. Light: Science & Applications, 7, 716-724 (2018).
DOI：

Whang, K. et al. Plasmonic bacteria on a nanoporous mirror via hydrodynamic trapping for rapid identification of waterborne pathogens. Light: Science & Applications, 7, 716-724 (2018). DOI： 10.1038/s41377-018-0071-4.

摘要

Abstract

A rapid, precise method for identifying waterborne pathogens is critically needed for effective disinfection and better treatment. However, conventional methods, such as culture-based counting, generally suffer from slow detection times and low sensitivities. Here, we developed a rapid detection method for tracing waterborne pathogens by an innovative optofluidic platform, a plasmonic bacteria on a nanoporous mirror, that allows effective hydrodynamic cell trapping, enrichment of pathogens, and optical signal amplifications. We designed and simulated the integrated optofluidic platform to maximize the enrichment of the bacteria and to align bacteria on the nanopores and plasmonic mirror via hydrodynamic cell trapping. Gold nanoparticles are self-assembled to form antenna arrays on the surface of bacteria, such as,Escherichia coli, and ,Pseudomonas aeruginosa, by replacing citrate with hydroxylamine hydrochloride in order to amplify the signal of the plasmonic optical array. Owing to the synergistic contributions of focused light via the nanopore geometry, self-assembled nanoplasmonic optical antennas on the surface of bacteria, and plasmonic mirror, we obtain a sensitivity of detecting ,E. coli, as low as 10,2, cells/ml via surface-enhanced Raman spectroscopy. We believe that our label-free strategy via an integrated optofluidic platform will pave the way for the rapid, precise identification of various pathogens.

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references

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