Near-resonance enhanced label-free stimulated Raman scattering microscopy with spatial resolution near 130 nm

Yali Bi; Chi Yang; Yage Chen; Shuai Yan; Guang Yang; Yaozu Wu; Guoping Zhang; Ping Wang

doi:10.1038/s41377-018-0082-1

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Near-resonance enhanced label-free stimulated Raman scattering microscopy with spatial resolution near 130 nm

Original Articles

- Near-resonance enhanced label-free stimulated Raman scattering microscopy with spatial resolution near 130 nm
- Light: Science & Applications Vol. 7, Issue 6, Pages: 811-820(2018)
- Affiliations：
  
  1.Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China
  2.MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China
  3.MoE Key Laboratory of Quark and Lepton Physics and College of Physics Science and Technology, Central China Normal University, Wuhan, 430079, China
- Author bio：
  
  Guoping Zhang (gpzhang@mail.ccnu.edu.cn)
  Ping Wang (p_wang@hust.edu.cn)
- Funds：
- DOI：10.1038/s41377-018-0082-1
  CLC：
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Bi, Y. L. et al. Near-resonance enhanced label-free stimulated Raman scattering microscopy with spatial resolution near 130 nm. Light: Science & Applications, 6, 811-820 (2018).
DOI：

Bi, Y. L. et al. Near-resonance enhanced label-free stimulated Raman scattering microscopy with spatial resolution near 130 nm. Light: Science & Applications, 6, 811-820 (2018). DOI： 10.1038/s41377-018-0082-1.

摘要

Abstract

High-resolution optical microscopes that can break 180 nm in spatial resolution set to conventional microscopies are much-needed tools. However, current optical microscopes have to rely on exogenous fluorescent labels to achieve high resolution in biological imaging. Herein, we report near-resonance enhanced label-free stimulated Raman scattering (SRS) microscopy with a lateral resolution near 130 nm, in which the high-resolution image contrast originates directly from a low concentration of endogenous biomolecules, with sensitivity gains of approximately 23 times. Moreover, by using a 0.3-m-long optical fiber, we developed hyperspectral SRS microscopy based on spectral focusing technology. Attributed to enhancements in spatial resolution and sensitivity, we demonstrated high-resolution imaging of three-dimensional structures in single cells and high-resolution mapping of large-scale intact mouse brain tissues in situ. By using enhanced high-resolution hyperspectral SRS, we chemically observed sphingomyelin distributed in the myelin sheath that insulates single axons. Our concept opens the door to biomedical imaging with ~130 nm resolution.

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references

Yang, Y. L. et al. Differential diagnosis of breast cancer using quantitative, label-free and molecular vibrational imaging. Biomed. Opt. Express 2, 2160–2174 (2011).

Mittal, R. et al. Evaluation of stimulated Raman scattering microscopy for identifying squamous cell carcinoma in human skin. Lasers Surg. Med. 45, 496–502 (2013).

Uckermann, O. et al. Label-free delineation of brain tumors by coherent anti-stokes Raman scattering microscopy in an orthotopic mouse model and human glioblastoma. PLoS ONE 9, e107115 (2014).

Hering, K. et al. SERS: a versatile tool in chemical and biochemical diagnostics. Anal. Bioanal. Chem. 390, 113–124 (2008).

Kim, H., Bryant, G. W. & Stranick, S. J. Superresolution four-wave mixing microscopy. Opt. Express 20, 6042–6051 (2012).

Wang, Y. et al. Wide-field, surface-sensitive four-wave mixing microscopy of nanostructures. Appl. Opt. 51, 3305–3312 (2012).

Liu, X. J., Wang, Y. & Potma, E. O. Surface-mediated four-wave mixing of nanostructures with counterpropagating surface plasmon polaritons. Opt. Lett. 36, 2348–2350 (2011).

Zumbusch, A., Holtom, G. R. & Xie, X. S. Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering. Phys. Rev. Lett. 82, 4142–4145 (1999).

Evans, C. L. & Xie, X. S. Coherent anti-Stokes Raman scattering microscopy: chemical imaging for biology and medicine. Annu Rev. Anal. Chem. 1, 883–909 (2008).

Camp, C. H. Jr et al. High-speed coherent Raman fingerprint imaging of biological tissues. Nat. Photonics 8, 627–634 (2014).

Yampolsky, S. et al. Seeing a single molecule vibrate through time-resolved coherent anti-Stokes Raman scattering. Nat. Photonics 8, 650–656 (2014).

Liu, W. & Niu, H. B. Diffraction barrier breakthrough in coherent anti-Stokes Raman scattering microscopy by additional probe-beam-induced phonon depletion. Phys. Rev. A 83, 023830 (2011).

Kawata S., Ichimura T., Hayazawa N., Hashimoto M., Inouye Y. Tip-enhanced near-field CARS microscopy for molecular nano-imaging. Proceedings of SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V. 5700: 52–59 (SPIE, San Jose, 2005).

Cheng, J. X. & Xie, X. S. Vibrational spectroscopic imaging of living systems: an emerging platform for biology and medicine. Science 350, aaa8870 (2015).

Freudiger, C. W. et al. Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy. Science 322, 1857–1861 (2008).

Hell, S. W. Toward fluorescence nanoscopy. Nat. Biotechnol. 21, 1347–1355 (2003).

Duncan, M. D., Reintjes, J. & Manuccia, T. J. Scanning coherent anti-Stokes Raman microscope. Opt. Lett. 7, 350–352 (1982).

Houston, W. V. A compound interferometer for fine structure work. Phys. Rev. 29, 478–484 (1927).

Hell, S. W. Far-field optical nanoscopy. Science 316, 1153–1158 (2007).

Allen, K. W. et al. Overcoming the diffraction limit of imaging nanoplasmonic arrays by microspheres and microfibers. Opt. Express 23, 24484–24496 (2015).

Fu, D. et al. Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy. J. Am. Chem. Soc. 134, 3623–3626 (2012).

Ozeki, Y. et al. High-speed molecular spectral imaging of tissue with stimulated Raman scattering. Nat. Photonics 6, 845–851 (2012).

Fu, D., Holtom, G., Freudiger, C., Zhang, X. & Xie, X. S. Hyperspectral imaging with stimulated Raman scattering by chirped femtosecond lasers. J. Phys. Chem. B 117, 4634–4640 (2013).

Paxinos G., Franklin K. B. J. The Mouse Brain in Stereotaxic Coordinates. 2nd edn. (Academic Press, San Diego, 2001).

Ji, M. B. et al. Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy. Sci. Transl. Med. 5, 201ra119 (2013).

Fu, D. et al. In vivo metabolic fingerprinting of neutral lipids with hyperspectral stimulated Raman scattering microscopy. J. Am. Chem. Soc. 136, 8820–8828 (2014).

Heinrich, C. et al. Selective imaging of saturated and unsaturated lipids by wide-field CARS-microscopy. Opt. Express 16, 2699–2708 (2008).

Di Napoli, C. et al. Quantitative spatiotemporal chemical profiling of individual lipid droplets by hyperspectral CARS microscopy in living human adipose-derived stem cells.Anal. Chem. 88, 3677–3685 (2016).

Slipchenko, M. N., Le, T. T., Chen, H. T. & Cheng, J. X. High-speed vibrational imaging and spectral analysis of lipid bodies by compound Raman microscopy. J. Phys. Chem. B 113, 7681–7686 (2009).

Rinia, H. A., Burger, K. N. J., Bonn, M. & Müller, M. Quantitative label-free imaging of lipid composition and packing of individual cellular lipid droplets using multiplex CARS microscopy. Biophys. J. 95, 4908–4914 (2008).

Wu, H. W. et al. In vivo lipidomics using single-cell Raman spectroscopy. Proc. Natl Acad. Sci. USA 108, 3809–3814 (2011).

Krafft, C., Neudert, L., Simat, T. & Salzer, R. Near infrared Raman spectra of human brain lipids. Spectrochim. Acta A Mol. Biomol. Spectrosc. 61, 1529–1535 (2005).

Wei, L. et al. Super-multiplex vibrational imaging. Nature 544, 465–470 (2017).

Albrecht, A. C. & Hutley, M. C. On the dependence of vibrational Raman intensity on the wavelength of incident light. J. Chem. Phys. 55, 4438–4443 (1971).

Asher, S. A. UV resonance Raman studies of molecular structure and dynamics: applications in physical and biophysical chemistry. Annu. Rev. Phys. Chem. 39, 537–588 (1988).

Held P. Quantitation of Peptides and Amino Acids with a Synergy™ HT Using UV Fluorescence (B.-T. Instruments, Winooski, 2003).

Jin, C., Nadakuditi, R. R., Michielssen, E. & Rand, S. C. Iterative, backscatter-analysis algorithms for increasing transmission and focusing light through highly scattering random media. J. Opt. Soc. Am. A 30, 1592–1602 (2013).

Han, Y. B., Li, M. H., Qiu, F. W., Zhang, M. & Zhang, Y. H. Cell-permeable organic fluorescent probes for live-cell long-term super-resolution imaging reveal lysosome-mitochondrion interactions. Nat. Commun. 8, 1307 (2017).

Pan, D. et al. A general strategy for developing cell-permeable photo-modulatable organic fluorescent probes for live-cell super-resolution imaging. Nat. Commun. 5, 5573 (2014).

Matsuzaki, F., Shirane, M., Matsumoto, M. & Nakayama, K. I. Protrudin serves as an adaptor molecule that connects KIF5 and its cargoes in vesicular transport during process formation. Mol. Biol. Cell 22, 4602–4620 (2011).

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