High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization

Wei Zhang; Yanxiu Li; Van Phuc Nguyen; Ziyi Huang; Zhipeng Liu; Xueding Wang; Yannis M. Paulus

doi:10.1038/s41377-018-0093-y

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High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization

Original Articles

- High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization
- Light: Science & Applications Vol. 7, Issue 6, Pages: 956-967(2018)
- Affiliations：
  
  1.Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48105, USA
  2.Institution of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, China
  3.Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI, 48105, USA
  4.Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China
  5.Department of Radiology, University of Michigan, Ann Arbor, MI, 48105, USA
- Author bio：
  
  Zhipeng Liu (lzpeng67@163.com)
  Zhipeng Liu (lzpeng67@163.com)
  Zhipeng Liu (lzpeng67@163.com)
- Funds：
- DOI：10.1038/s41377-018-0093-y
  CLC：
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Zhang, W. et al. High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization. Light: Science & Applications, 7, 956-967 (2018).
DOI：

Zhang, W. et al. High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization. Light: Science & Applications, 7, 956-967 (2018). DOI： 10.1038/s41377-018-0093-y.

摘要

Abstract

Photoacoustic microscopy (PAM) is an emerging imaging technology that can non-invasively visualize ocular structures in animal eyes. This report describes an integrated multimodality imaging system that combines PAM, optical coherence tomography (OCT), and fluorescence microscopy (FM) to evaluate angiogenesis in larger animal eyes. High-resolution in vivo imaging was performed in live rabbit eyes with vascular endothelial growth factor (VEGF)-induced retinal neovascularization (RNV). The results demonstrate that our multimodality imaging system can non-invasively visualize RNV in both albino and pigmented rabbits to determine retinal pathology using PAM and OCT and verify the leakage of neovascularization using FM and fluorescein dye. This work presents high-resolution visualization of angiogenesis in rabbits using a multimodality PAM, OCT, and FM system and may represent a major step toward the clinical translation of the technology.

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references

Campochiaro, P. A. Molecular pathogenesis of retinal and choroidal vascular diseases. Prog. Retin. Eye Res. 49, 67-81 (2015).

Al-Shabrawey, M. et al. Targeting neovascularization in ischemic retinopathy: recent advances. Expert. Rev. Ophthalmol. 8, 267-286 (2013).

Campochiaro, P. A. Ocular neovascularization. J. Mol. Med. 91, 311-321 (2013).

Brand, C. S. Management of retinal vascular diseases: a patient-centric approach. Eye 26, S1-S16 (2012).

Harvey, P. T. Common eye diseases of elderly people: identifying and treating causes of vision loss.Gerontology 49, 1-11 (2003).

Lovett, M., Lee, K., Edwards, A. & Kaplan, D. L. Vascularization strategies for tissue engineering. Tissue Eng. Part B Rev. 15, 353-370 (2009).

Ferris, F. L. Ⅲ & Patz, A. Macular edema: a major complication of diabetic retinopathy. Trans. New Orleans Acad. Ophthalmol. 31, 307-316 (1983).

Kim, J., Brown, W., Maher, J. R., Levinson, H. & Wax, A. Functional optical coherence tomography: principles and progress. Phys. Med. Biol. 60, R211-R237 (2015).

Ng, E. Y., Lanigan, B. & O'Keefe, M. Fundus fluorescein angiography in the screening for and management of retinopathy of prematurity. J. Pediatr. Ophthalmol. Strabismus 43, 85-90 (2006).

Yang, C. Molecular contrast optical coherence tomography: a review. Photochem. Photobiol. 81, 215-237 (2005).

Xu, M. & Wang, L. V. Photoacoustic imaging in biomedicine. Rev. Sci. Instrum. 77, 041101 (2006).

Laufer, J. G. et al. In vivo preclinical photoacoustic imaging of tumor vasculature development and therapy. J. Biomed. Opt. 17, 056016 (2012).

Yang, S. H. et al. Noninvasive monitoring of traumatic brain injury and post-traumatic rehabilitation with laser-induced photoacoustic imaging. Appl. Phys. Lett. 90, 243902 (2007).

Wang, X. D., Pang, Y. J., Ku, G., Stoica, G. & Wang, L. V. Three-dimensional laser-induced photoacoustic tomography of mouse brain with the skin and skull intact. Opt. Lett. 28, 1739-1741 (2003).

Rajian, J. R., Girish, G. & Wang, X. D. Photoacoustic tomography to identify inflammatory arthritis. J. Biomed. Opt. 17, 096013 (2012).

Mitcham, T., Dextraze, K., Taghavi, H., Melancon, M. & Bouchard, R. Photoacoustic imaging driven by an interstitial irradiation source. Photoacoustics 3, 45-54 (2015).

Jo, J. et al. A functional study of human inflammatory arthritis using photoacoustic imaging. Sci. Rep. 7, 15026 (2017).

Xia, J., Yao, J. J. & Wang, L. V. Photoacoustic tomography: principles and advances. Electromagn. Waves 147, 1-22 (2014).

de La Zerda, A. et al. Photoacoustic ocular imaging. Opt. Lett. 35, 270-272 (2010).

Hu, S., Rao, B., Maslov, K. & Wang, L. V. Label-free photoacoustic ophthalmic angiography. Opt. Lett. 35, 1-3 (2010).

Jiao, S. L. et al. Photoacoustic ophthalmoscopy for in vivo retinal imaging. Opt. Express 18, 3967-3972 (2010).

Liu, W. Z. & Zhang, H. F. Photoacoustic imaging of the eye: a mini review. Photoacoustics 4, 112-123 (2016).

Tian, C., Zhang, W., Mordovanakis, A., Wang, X. D. & Paulus, Y. M. Noninvasive chorioretinal imaging in living rabbits using integrated photoacoustic microscopy and optical coherence tomography. Opt. Express 25, 15947-15955 (2017).

Tian, C., Zhang, W., Nguyen, V. P., Wang, X. D., Paulus, Y. M. Novel photoacoustic microscopy and optical coherence tomography dual-modality chorioretinal imaging in living rabbit eyes. J. Vis. Exp. 132, (2018) https://doi.org/10.3791/57135.

Liu, W. Z. et al. In vivo corneal neovascularization imaging by optical-resolution photoacoustic microscopy. Photoacoustics 2, 81-86 (2014).

Lin, R. Q. et al. Longitudinal label-free optical-resolution photoacoustic microscopy of tumor angiogenesis in vivo. Quant. Imaging Med. Surg. 5, 23-29 (2015).

Rabbani, H., Allingham, M. J., Mettu, P. S., Cousins, S. W. & Farsiu, S. Fully automatic segmentation of fluorescein leakage in subjects with diabetic macular edema. Invest. Ophthalmol. Vis. Sci. 56, 1482-1492 (2015).

Wang, T. X. et al. Multiparametric photoacoustic microscopy of the mouse brain with 300-kHz A-line rate. Neurophotonics 3, 045006 (2016).

Ma, T. et al. Systematic study of high-frequency ultrasonic transducer design for laser-scanning photoacoustic ophthalmoscopy. J. Biomed. Opt. 19, 016015 (2014).

Wong, C. G., Rich, K. A., Liaw, L. H. L., Hsu, H. T. & Berns, M. W. Intravitreal VEGF and bFGF produce florid retinal neovascularization and hemorrhage in the rabbit. Curr. Eye Res. 22, 140-147 (2001).

Grossniklaus, H. E., Kang, S. J. & Berglin, L. Animal models of choroidal and retinal neovascularization. Prog. Retin. Eye Res. 29, 500-519 (2010).

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