Sharpening emitter localization in front of a tuned mirror

Hannah S. Heil; Benjamin Schreiber; Ralph Götz; Monika Emmerling; Marie-Christine Dabauvalle; Georg Krohne; Sven Höfling; Martin Kamp; Markus Sauer; Katrin G. Heinze

doi:10.1038/s41377-018-0104-z

Go to Nature Website

Your Location：

Home >

Browse articles >

Sharpening emitter localization in front of a tuned mirror

Letter

- Sharpening emitter localization in front of a tuned mirror
- Light: Science & Applications Vol. 7, Issue 6, Pages: 671-678(2018)
- Affiliations：
  
  1.Rudolf Virchow Center, Research Center for Experimental Biomedicine, University of Würzburg, Josef-Schneider-Str.2, 97080, Würzburg, Germany
  2.Department of Biotechnology and Biophysics, Biozentrum, University of Würzburg, Am Hubland, 97074, Würzburg, Germany
  3.Technische Physik, Physikalisches Institut and Wilhelm Conrad Röntgen-Center for Complex Material Systems, University of Würzburg, Am Hubland, 97074, Würzburg, Germany
  4.Division of Electron Microscopy, Biozentrum, University of Würzburg, Am Hubland, 97074, Würzburg, Germany
  5.SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, UK
- Author bio：
  
  Markus Sauer (m.sauer@uni-wuerzburg.de)
  Katrin G. Heinze (katrin.heinze@virchow.uni-wuerzburg.de)
- Funds：
- DOI：10.1038/s41377-018-0104-z
  CLC：
Scan for full text
Heil, H. S. et al. Sharpening emitter localization in front of a tuned mirror. Light: Science & Applications, 7, 671-678 (2018).
DOI：

Heil, H. S. et al. Sharpening emitter localization in front of a tuned mirror. Light: Science & Applications, 7, 671-678 (2018). DOI： 10.1038/s41377-018-0104-z.

摘要

Abstract

Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio,1,1,. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror,2,2,-,4,4,2-4,., Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.

关键词

Keywords

references

Sauer, M. & Heilemann, M. Single-molecule localization microscopy in eukaryotes. Chem. Rev. 117, 7478–7509 (2017).

Bailey, B., Farkas, D. L., Taylor, D. L. & Lanni, F. Enhancement of axial resolution in fluorescence microscopy by standing-wave excitation. Nature 366, 44–48 (1993).

Drexhage, K. H., Kuhn, H. & Schäfer, F. P. Variation of the fluorescence decay time of a molecule in front of a mirror. Ber. Bunsen-Ges. Phys. Chem. 72, 329 (1968).

Le Moal, E. et al. Enhanced fluorescence cell imaging with metal-coated slides. Biophys. J. 92, 2150–2161 (2007).

Drexhage, K. H. Interaction of light with monomolecular dye layers. Prog. Opt. 12, 163–192 (1974).

Chance, R. R., Prock, A. & Silbey, R. Molecular fluorescence and energy transfer near interfaces. Adv. Chem. Phys. 37, 1–65 (1978).

Yang, X. S. et al. Mirror-enhanced super-resolution microscopy.Light Sci. Appl. 5, e16134 (2016).

Betzig, E. et al. Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 1642–1645 (2006).

Rust, M. J., Bates, M. & Zhuang, X. W. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat. Methods 3, 793–796 (2006).

Heilemann, M. et al. Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes. Angew. Chem. Int. Ed. Engl. 47, 6172–6176 (2008).

Jungmann, R. et al. Single-molecule kinetics and super-resolution microscopy by fluorescence imaging of transient binding on DNA origami. Nano Lett. 10, 4756–4761 (2010).

Quan, T. W., Zeng, S. Q. & Huang, Z. L. Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide semiconductor, and charge-coupled devices for superresolution imaging. J. Biomed. Opt. 15, 066005 (2010).

Grimm, J. B. et al. A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12, 244–250 (2015).

Altman, R. B. et al. Cyanine fluorophore derivatives with enhanced photostability. Nat. Methods 9, 68–71 (2011).

Li, W. X., Stein, S. C., Gregor, I. & Enderlein, J. Ultra-stable and versatile widefield cryo-fluorescence microscope for single-molecule localization with sub-nanometer accuracy. Opt. Express 23, 3770–3783 (2015).

Xu, K., Babcock, H. P. & Zhuang, X. W. Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton. Nat. Methods 9, 185–188 (2012).

Strambio-De-Castillia, C., Niepel, M. & Rout, M. P. The nuclear pore complex: bridging nuclear transport and gene regulation. Nat. Rev. Mol. Cell Biol. 11, 490–501 (2010).

Löschberger, A. et al. Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution.J. Cell Sci. 125, 570–575 (2012).

Göttfert, F. et al. Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent. Proc. Natl. Acad. Sci. USA 114, 2125–2130 (2017).

Elsayad, K. et al. Spectrally coded optical nanosectioning (SpecON) with biocompatible metal-dielectric-coated substrates. Proc. Natl. Acad. Sci. USA 110, 20069–20074 (2013).

Nieuwenhuizen, R. P. J. et al. Measuring image resolution in optical nanoscopy. Nat. Methods 10, 557–562 (2013).

Franke, C., Sauer, M. & van de Linde, S. Photometry unlocks 3D information from 2D localization microscopy data. Nat. Methods 14, 41–44 (2017).

Takeda, A., Wu, J. J. & Maizel, A. L. Evidence for monomeric and dimeric forms of CD45 associated with a 30-kDa phosphorylated protein. J. Biol. Chem. 267, 16651–16659 (1992).

Cabriel, C., Bourg, N., Dupuis, G. & Lévêque-Fort, S. Aberration-accounting calibration for 3D single-molecule localization microscopy. Opt. Lett. 43, 174–177 (2018).

Chizhik, A. I., Rother, J., Gregor, I., Janshoff, A. & Enderlein, J. Metal-induced energy transfer for live cell nanoscopy. Nat. Photonics 8, 124–127 (2014).

Karedla, N. et al. Three-dimensional single-molecule localization with nanometer accuracy using Metal-Induced Energy Transfer (MIET) imaging. J. Chem. Phys. 148, 204201 (2018).

Ropp, C. et al. Nanoscale probing of image-dipole interactions in a metallic nanostructure. Nat. Commun. 6, 6558 (2015).

Raab, M., Vietz, C., Stefani, F. D., Acuna, G. P. & Tinnefeld, P. Shifting molecular localization by plasmonic coupling in a single-molecule mirage. Nat. Commun. 8, 13966 (2017).

Mack, D. L. et al. Decoupling absorption and emission processes in super-resolution localization of emitters in a plasmonic hotspot. Nat. Commun. 8, 14513 (2017).

Schreiber, B. et al. Enhanced fluorescence resonance energy transfer in G-pro-tein-coupled receptor probes on nanocoated microscopy coverslips. ACS Photonics 5, 2225–2233 (2018).

Douglass, K. M., Sieben, C., Archetti, A., Lambert, A. & Manley, S. Super-resolution imaging of multiple cells by optimized flat-field epi-illumination. Nat. Photonics 10, 705–708 (2016).

Schreiber, B., Elsayad, K. & Heinze, K. G. Axicon-based Bessel beams for flat-field illumination in total internal reflection fluorescence microscopy. Opt. Lett. 42, 3880–3883 (2017).

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰