Full-field interferometric imaging of propagating action potentials

Tong Ling; Kevin C. Boyle; Georges Goetz; Peng Zhou; Yi Quan; Felix S. Alfonso; Tiffany W. Huang; Daniel Palanker

doi:10.1038/s41377-018-0107-9

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Full-field interferometric imaging of propagating action potentials

Original Articles

- Full-field interferometric imaging of propagating action potentials
- Light: Science & Applications Vol. 7, Issue 6, Pages: 1008-1018(2018)
- Affiliations：
  
  1.Hansen Experimental Physics Laboratory, Stanford University, Stanford, CA, 94305, USA
  2.Department of Ophthalmology, Stanford University, Stanford, CA, 94305, USA
  3.Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA
  4.Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University, Stanford, CA, 94305, USA
  5.Department of Chemistry, Stanford University, Stanford, CA, 94305, USA
- Author bio：
  
  Tong Ling (tongling@stanford.edu)
  Daniel Palanker (palanker@stanford.edu)
- Funds：
- DOI：10.1038/s41377-018-0107-9
  CLC：
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Ling, T. et al. Full-field interferometric imaging of propagating action potentials. Light: Science & Applications, 7, 1008-1018 (2018).
DOI：

Ling, T. et al. Full-field interferometric imaging of propagating action potentials. Light: Science & Applications, 7, 1008-1018 (2018). DOI： 10.1038/s41377-018-0107-9.

摘要

Abstract

Currently, cellular action potentials are detected using either electrical recordings or exogenous fluorescent probes that sense the calcium concentration or transmembrane voltage. Ca imaging has a low temporal resolution, while voltage indicators are vulnerable to phototoxicity, photobleaching, and heating. Here, we report full-field interferometric imaging of individual action potentials by detecting movement across the entire cell membrane. Using spike-triggered averaging of movies synchronized with electrical recordings, we demonstrate deformations up to 3 nm (0.9 mrad) during the action potential in spiking HEK-293 cells, with a rise time of 4 ms. The time course of the optically recorded spikes matches the electrical waveforms. Since the shot noise limit of the camera (~2 mrad/pix) precludes detection of the action potential in a single frame, for all-optical spike detection, images are acquired at 50 kHz, and 50 frames are binned into 1 ms steps to achieve a sensitivity of 0.3 mrad in a single pixel. Using a self-reinforcing sensitivity enhancement algorithm based on iteratively expanding the region of interest for spatial averaging, individual spikes can be detected by matching the previously extracted template of the action potential with the optical recording. This allows all-optical full-field imaging of the propagating action potentials without exogeneous labels or electrodes.

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references

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