Multiplexed laser particles for spatially resolved single-cell analysis

Sheldon J. J. Kwok; Nicola Martino; Paul H. Dannenberg; Seok-Hyun Yun

doi:10.1038/s41377-019-0183-5

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Multiplexed laser particles for spatially resolved single-cell analysis

Perspectives

- Multiplexed laser particles for spatially resolved single-cell analysis
- Light: Science & Applications Vol. 8, Issue 5, Pages: 649-653(2019)
- Affiliations：
  
  1.Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 50 Blossom Street, Boston, MA 02114, USA
  2.Harvard-MIT Health Sciences and Technology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
  3.LASE Innovation Inc., 650 E. Kendall Street, 2nd FL, Cambridge, MA 02142, USA
- Author bio：
  
  Seok-Hyun Yun (syun@hms.harvard.edu)
- Funds：
- DOI：10.1038/s41377-019-0183-5
  CLC：
- Published：2019，
  
  Published Online：21 August 2019，
  
  Received：02 March 2019，
  
  Revised：11 July 2019，
  
  Accepted：22 July 2019
- Accepted：
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Kwok, S. J. J. et al. Multiplexed laser particles for spatially resolved single-cell analysis. Light: Science & Applications, 8, 649-653 (2019).
DOI：

Kwok, S. J. J. et al. Multiplexed laser particles for spatially resolved single-cell analysis. Light: Science & Applications, 8, 649-653 (2019). DOI： 10.1038/s41377-019-0183-5.

摘要

Abstract

Biomolecular analysis at the single-cell level is increasingly important in the study of cellular heterogeneity and its consequences

particularly in organismic development and complex diseases such as cancer. Single-cell molecular analyses have led to the identification of new cell types

and the discovery of novel targets for diagnosis and therapy

. While these analyses are performed predominantly on dissociated single cells

emerging techniques seek understanding of cellular state

cellular function and cell-cell interactions within the native tissue environment by combining optical microscopy and single-cell molecular analyses. These techniques include in situ multiplexed imaging of fluorescently labeled proteins and nucleotides

as well as low-throughput ex vivo methods in which specific cells are isolated for downstream molecular analyses. However

these methods are limited in either the number and type of molecular species they can identify or the number of cells that ca

n be analyzed. High-throughput methods are needed for comprehensive profiling of many cells (

1000) to detect rare cell types

discriminate relevant biomarkers from intrinsic population noise

and reduce the time and cost of measurement. Many established

high-throughput single-cell analyses are not directly applicable because they require tissue dissociation

leading to a loss of spatial information

. No current methods exist that can seamlessly connect spatial mapping to single-cell techniques. In this Perspective

we review current methods for spatially resolved single-cell analysis and discuss the prospect of novel multiplexed imaging probes

called laser particles

which allow individual cells to be tagged in tissue and analyzed subsequently using high-throughput

comprehensive single-cell techniques.

关键词

Keywords

references

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