Colour compound lenses for a portable fluorescence microscope

Bo Dai; Ziao Jiao; Lulu Zheng; Hunter Bachman; Yongfeng Fu; Xinjun Wan; Yule Zhang; Yu Huang; Xiaodian Han; Chenglong Zhao; Tony Jun Huang; Songlin Zhuang; Dawei Zhang

doi:10.1038/s41377-019-0187-1

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Colour compound lenses for a portable fluorescence microscope

Articles

- Colour compound lenses for a portable fluorescence microscope
- Light: Science & Applications Vol. 8, Issue 5, Pages: 734-746(2019)
- Affiliations：
  
  1.Engineering Research Center of Optical Instrument and System, the Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, 200093 Shanghai, China
  2.Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27709, USA
  3.Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, 200032 Shanghai, China
  4.Department of Laboratory Medicine, Shanghai Cancer Center, Fudan University, 200032 Shanghai, China
  5.Department of Physics, University of Dayton, Dayton, OH 45469, USA
  6.Department of Electro-Optics and Photonics, University of Dayton, Dayton, OH 45469, USA
- Author bio：
  
  Tony Jun Huang (tony.huang@duke.edu)
  Dawei Zhang (dwzhang@usst.edu.cn)
- Funds：
- DOI：10.1038/s41377-019-0187-1
  CLC：
- Published：2019，
  
  Published Online：21 August 2019，
  
  Received：04 March 2019，
  
  Revised：11 July 2019，
  
  Accepted：31 July 2019
- Accepted：
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Dai, B. et al. Colour compound lenses for a portable fluorescence microscope. Light: Science & Applications, 8, 734-746 (2019).
DOI：

Dai, B. et al. Colour compound lenses for a portable fluorescence microscope. Light: Science & Applications, 8, 734-746 (2019). DOI： 10.1038/s41377-019-0187-1.

摘要

Abstract

In this article

we demonstrated a handheld smartphone fluorescence microscope (HSFM) that integrates dual-functional polymer lenses with a smartphone. The HSFM consists of a smartphone

a field-portable illumination source

and a dual-functional polymer lens that performs both optical imaging and filtering. Therefore

compared with the existing smartphone fluorescence microscope

the HSFM does not need any additional optical filters. Although fluorescence imaging has traditionally played an indispensable role in biomedical and clinical applications due to its high specificity and sensitivity for detecting cells

proteins

DNAs/RNAs

etc.

the bulky elements of conventional fluorescence microscopes make them inconvenient for use in point-of-care diagnosis. The HSFM demonstrated in this article solves this problem by providing a multifunctional

miniature

small-form-factor fluorescence module. This multifunctional fluorescence module can be seamlessly attached to any smartphone camera for both bright-field and fluorescence imaging at cellular-scale resolutions without the use of additional bulky lenses/filters; in fact

the HSFM achieves magnification and light filtration using a single lens. Cell and tissue observation

cell counting

plasmid transfection evaluation

and superoxide production analysis were performed using this device. Notably

this lens system has the unique capability of functioning with numerous smartphones

irrespective of the smartphone model and the camera technology housed within each device. As such

this HSFM has the potential to pave the way for real-time point-of-care diagnosis and opens up countless possibilities for personalized medicine.

关键词

Keywords

references

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