3D cellular-resolution imaging in arteries using few-mode interferometry

Biwei Yin; Zhonglie Piao; Kensuke Nishimiya; Chulho Hyun; Joseph A. Gardecki; Adam Mauskapf; Farouc A. Jaffer; Guillermo J. Tearney

doi:10.1038/s41377-019-0211-5

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3D cellular-resolution imaging in arteries using few-mode interferometry

Article

- 3D cellular-resolution imaging in arteries using few-mode interferometry
- Light: Science & Applications Vol. 8, Issue 6, Pages: 1044-1052(2019)
- Affiliations：
  
  1.Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, Boston, MA 02114, USA
  2.Cardiovascular Research Center and Cardiology Division, Harvard Medical School and Massachusetts General Hospital, Boston, MA 02114, USA
  3.Department of Pathology, Harvard Medical School and Massachusetts General Hospital, Boston, MA 02114, USA
  4.Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA
- Author bio：
  
  Guillermo J. Tearney (gtearney@partners.org)
- Funds：
- DOI：10.1038/s41377-019-0211-5
  CLC：
- Published：2019，
  
  Published Online：21 November 2019，
  
  Received：26 June 2019，
  
  Revised：15 October 2019，
  
  Accepted：29 October 2019
- Accepted：
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Yin, B. W. et al. 3D cellular-resolution imaging in arteries using few-mode interferometry. Light: Science & Applications, 8, 1044-1052 (2019).
DOI：

Yin, B. W. et al. 3D cellular-resolution imaging in arteries using few-mode interferometry. Light: Science & Applications, 8, 1044-1052 (2019). DOI： 10.1038/s41377-019-0211-5.

摘要

Abstract

Cross-sectional visualisation of the cellular and subcellular structures of human atherosclerosis in vivo is significant

as this disease is fundamentally caused by abnormal processes that occur at this scale in a depth-dependent manner. However

due to the inherent resolution-depth of focus tradeoff of conventional focusing optics

today's highest-resolution intravascular imaging technique

namely

optical coherence tomography (OCT)

is unable to provide cross-sectional images at this resolution through a coronary catheter. Here

we introduce an intravascular imaging system and catheter based on few-mode interferometry

which overcomes the depth of focus limitation of conventional high-numerical-aperture objectives and enables three-dimensional cellular-resolution intravascular imaging in vivo by a submillimetre diameter

flexible catheter. Images of diseased cadaver human coronary arteries and living rabbit arteries were acquired with this device

showing clearly resolved cellular and subcellular structures within the artery wall

such as individual crystals

smooth muscle cells

and inflammatory cells. The capability of this technology to enable cellular-resolution

cross-sectional intravascular imaging will make it possible to study and diagnose human coronary disease with much greater precision in the future.

关键词

Keywords

references

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