Super-resolution localization photoacoustic microscopy using intrinsic red blood cells as contrast absorbers

Jongbeom Kim; Jin Young Kim; Seungwan Jeon; Jin Woo BAIK; Seong Hee Cho; Chulhong Kim

doi:10.1038/s41377-019-0220-4

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Super-resolution localization photoacoustic microscopy using intrinsic red blood cells as contrast absorbers

Article

- Super-resolution localization photoacoustic microscopy using intrinsic red blood cells as contrast absorbers
- Light: Science & Applications Vol. 8, Issue 6, Pages: 1033-1043(2019)
- Affiliations：
  
  Departments of Creative IT Engineering, Electrical Engineering, Mechanical Engineering, and Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea
- Author bio：
  
  Chulhong Kim (chulhong@postech.edu)
- Funds：
- DOI：10.1038/s41377-019-0220-4
  CLC：
- Published：2019，
  
  Published Online：20 November 2019，
  
  Received：26 July 2019，
  
  Revised：14 October 2019，
  
  Accepted：04 November 2019
- Accepted：
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Kim, J. B. et al. Super-resolution localization photoacoustic microscopy using intrinsic red blood cells as contrast absorbers. Light: Science & Applications, 8, 1033-1043 (2019).
DOI：

Kim, J. B. et al. Super-resolution localization photoacoustic microscopy using intrinsic red blood cells as contrast absorbers. Light: Science & Applications, 8, 1033-1043 (2019). DOI： 10.1038/s41377-019-0220-4.

摘要

Abstract

Photoacoustic microscopy (PAM) has become a premier microscopy tool that can provide the anatomical

functional

and molecular information of animals and humans in vivo. However

conventional PAM systems suffer from limited temporal and/or spatial resolution. Here

we present a fast PAM system and an agent-free localization method based on a stable and commercial galvanometer scanner with a custom-made scanning mirror (L-PAM-GS). This novel hardware implementation enhances the temporal resolution significantly while maintaining a high signal-to-noise ratio (SNR). These improvements allow us to photoacoustically and noninvasively observe the microvasculatures of small animals and humans in vivo. Furthermore

the functional hemodynamics

namely

the blood flow rate in the microvasculature

is successfully monitored and quantified in vivo. More importantly

thanks to the high SNR and fast B-mode rate (500?Hz)

by localizing photoacoustic signals from captured red blood cells without any contrast agent

unresolved microvessels are clearly distinguished

and the spatial resolution is improved by a factor of 2.5 in vivo. L-PAM-GS has great potential in various fields

such as neurology

oncology

and pathology.

关键词

Keywords

references

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