Bond-selective transient phase imaging via sensing of the infrared photothermal effect

Delong Zhang; Lu Lan; Yeran Bai; Hassaan Majeed; Mikhail E. Kandel; Gabriel Popescu; Ji-Xin Cheng

doi:10.1038/s41377-019-0224-0

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Bond-selective transient phase imaging via sensing of the infrared photothermal effect

Article

- Bond-selective transient phase imaging via sensing of the infrared photothermal effect
- Light: Science & Applications Vol. 8, Issue 6, Pages: 1126-1137(2019)
- Affiliations：
  
  1.Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA
  2.Department of Physics, Zhejiang University, Hangzhou 310028, China
  3.National Laboratory on High Power Laser and Physics, Shanghai 201800, China
  4.Key Laboratory of High Power Laser and Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China
  5.Department of Bioengineering, University of Illinois at UrbanaChampaign, Champaign, IL 61801, USA
  6.Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Champaign, IL 61801, USA
  7.Department of Electrical & Computer Engineering, Boston University, Boston, MA 02215, USA
  8.Photonics Center, Boston University, Boston, MA 02215, USA
- Author bio：
  
  Gabriel Popescu (gpopescu@illinois.edu)
  Ji-Xin Cheng (jxcheng@bu.edu)
- Funds：
- DOI：10.1038/s41377-019-0224-0
  CLC：
- Published：2019，
  
  Published Online：11 December 2019，
  
  Received：25 July 2019，
  
  Revised：07 November 2019，
  
  Accepted：18 November 2019
- Accepted：
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Zhang, D. et al. Bond-selective transient phase imaging via sensing of the infrared photothermal effect. Light: Science & Applications, 8, 1126-1137 (2019).
DOI：

Zhang, D. et al. Bond-selective transient phase imaging via sensing of the infrared photothermal effect. Light: Science & Applications, 8, 1126-1137 (2019). DOI： 10.1038/s41377-019-0224-0.

摘要

Abstract

Phase-contrast microscopy converts the phase shift of light passing through a transparent specimen

e.g.

a biological cell

into brightness variations in an image. This ability to observe structures without destructive fixation or staining has been widely utilized for applications in materials and life sciences. Despite these advantages

phase-contrast microscopy lacks the ability to reveal molecular information. To address this gap

we developed a bond-selective transient phase (BSTP) imaging technique that excites molecular vibrations by infrared light

resulting in a transient change in phase shift that can be detected by a diffraction phase microscope. By developing a time-gated pump-probe camera system

we demonstrate BSTP imaging of live cells at a 50 Hz frame rate with high spectral fidelity

sub-microsecond temporal resolution

and sub-micron spatial resolution. Our approach paves a new way for spectroscopic imaging investigation in biology and materials science.

关键词

Keywords

references

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