By coupling dye molecules with a graphene layer and localizing the molecules through quantification of fluorescence lifetime quenching
a novel imaging system offers unprecedented 1-nm resolution with angstrom precision in the axial dimension.
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references
Balzarotti, F. et al. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes.Science355, 606–612 (2017)..
Ghosh, A. et al. Graphene-based metal-induced energy transfer for sub-nanometre optical localization.Nat. Photonics. https://doi.org/10.1038/s41566-019-0510-7 (2019).
Esa, A. et al. Three-dimensional spectral precision distance microscopy of chromatin nanostructures after triple-colour DNA labelling: a study of the BCR region on chromosome 22 and the Philadelphia chromosome.J. Microsc.199, 96–105 (2000)..
Zhanghao, K. et al. Super-resolution dipole orientation mapping via polarization demodulation.Light.: Sci. Appl.5, e16166 (2016)..
Schermelleh, L. et al. Super-resolution microscopy demystified.Nat. Cell Biol.21, 72–84 (2019)..
Lee, C. H.&Wang, J. Noninterferometric differential confocal microscopy with 2-nm depth resolution.Opt. Commun.135, 233–237 (1997)..
Taylor, R. W. et al. Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane.Nat. Photonics13, 480–487 (2019)..
Chizhik, A. I. et al. Metal-induced energy transfer for live cell nanoscopy.Nat. Photonics8, 124–127 (2014)..
Eibl, M. et al. Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate.Biomed. Opt. Express8, 3132–3142 (2017)..