A Trojan Horse for live-cell super-resolution microscopy

Beliu Gerti; Sauer Markus

doi:10.1038/s41377-019-0238-7

Go to Nature Website

Your Location：

Home >

Browse articles >

A Trojan Horse for live-cell super-resolution microscopy

News & Views

- A Trojan Horse for live-cell super-resolution microscopy
- Light: Science & Applications Vol. 9, Issue 1, Pages: 8-9(2020)
- Affiliations：
  
  Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany
- Author bio：
  
  Markus Sauer (m.sauer@uni-wuerzburg.de)
- Funds：
- DOI：10.1038/s41377-019-0238-7
  CLC：
- Published：2020，
  
  Published Online：06 January 2020，
- Accepted：
Scan QR Code
Beliu, G. & Sauer, M. A Trojan Horse for live-cell super-resolution microscopy. Light: Science & Applications, 9, 8–9 (2020).
DOI：

Beliu, G. & Sauer, M. A Trojan Horse for live-cell super-resolution microscopy. Light: Science & Applications, 9, 8–9 (2020). DOI： 10.1038/s41377-019-0238-7.

摘要

Abstract

New peptide vehicles enable the efficient live-cell labeling of intracellular organelles with cell-impermeable fluorescent probes by simple coincubation

paving the way for refined multicolor super-resolution fluorescence imaging.

关键词

Keywords

references

Sauer, M.&Heilemann, M. Single-molecule localization microscopy in eukaryotes.Chem. Rev.117, 7478-7509 (2017)..

Schermelleh, L. et al. Super-resolution microscopy demystified.Nat. Cell Biol.21, 72-81 (2019)..

Miller, L. W.&Cornish, V. W. Selective chemical labeling of proteins in living cells.Curr. Opin. Chem. Biol.9, 56-61 (2005)..

Fernández-Suárez, M.&Ting, A. Y. Fluorescent probes for super-resolution imaging in living cells.Nat. Rev. Mol. Cell Biol.9, 929-943 (2008)..

Sauer, M., Hofkens, J.&Enderlein, J. Handbook of Fluorescence Spectroscopy and Imaging: From Single Molecules to Ensembles. (Weinheim: Wiley-VCH, 2011).

Frankel, A. D.&Pabo, C. O. Cellular uptake of the tat protein from human immunodeficiency virus.Cell55, 1189-1193 (1988)..

Green, M.&Loewenstein, P. M. Autonomous functional domains of chemically synthesized human immunodeficiency virus tattrans-activator protein.Cell55, 1179-1188 (1988)..

Zhang, M. et al. Simple and efficient delivery of cell-impermeable organic fluorescent probes into live cells for live-cell superresolution imaging.Light.: Sci. Appl.8, 73 (2019)..

Lukinavičius, G. et al. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins.Nat. Chem.5, 132-139 (2013)..

Grimm, J. B. et al. A general method to improve fluorophores for live-cell and single-molecule microscopy.Nat. Methods12, 244-250 (2015)..

Grimm, J. B. et al. Bright photoactivatable fluorophores for single-molecule imaging.Nat. Methods13, 985-988 (2016)..

Bottanelli, F. et al. Two-colour live-cell nanoscale imaging of intracellular targets.Nat. Commun.7, 10778 (2016)..

Lukinavičius, G. Fluorogenic probes for live-cell imaging of the cytoskeleton.Nat. Methods11, 731-733 (2014)..

Erazo-Oliveras, A. et al. Protein delivery into live cells by incubation with an endosomolytic agent.Nat. Methods11, 861-867 (2014)..

Beliu, G. et al. Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy.Commun. Biol.2, 261 (2019)..

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰