Dynamic conjugate F-SHARP microscopy

Ioannis N. Papadopoulos; Jean-Sebastien Jouhanneau; Naoya Takahashi; David Kaplan; Matthew Larkum; James Poulet; Benjamin Judkewitz

doi:10.1038/s41377-020-00348-x

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Dynamic conjugate F-SHARP microscopy

Original Articles

- Dynamic conjugate F-SHARP microscopy
- Light: Science & Applications Vol. 9, Issue 4, Pages: 1084-1091(2020)
- Affiliations：
  
  1.Charité – Universitätsmedizin Berlin, Einstein Center for Neurosciences, NeuroCure Cluster of Excellence, Charitéplatz 1, 10117 Berlin, Germany
  2.Max Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, 13092 Berlin, Germany
  3.Institute for Biology, Humboldt University, Charitéplatz 1, 10117 Berlin, Germany
- Author bio：
  
  Benjamin Judkewitz (benjamin.judkewitz@charite.de)
- Funds：
- DOI：10.1038/s41377-020-00348-x
  CLC：
- Published：2020，
  
  Published Online：30 June 2020，
  
  Received：30 December 2019，
  
  Revised：01 June 2020，
  
  Accepted：15 June 2020
- Accepted：
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Papadopoulos, I. N. et al. Dynamic conjugate F-SHARP microscopy. Light: Science & Applications, 9, 1084-1091 (2020).
DOI：

Papadopoulos, I. N. et al. Dynamic conjugate F-SHARP microscopy. Light: Science & Applications, 9, 1084-1091 (2020). DOI： 10.1038/s41377-020-00348-x.

摘要

Abstract

Optical microscopy is an indispensable tool in biomedical sciences

but its reach in deep tissues is limited due to aberrations and scattering. This problem can be overcome by wavefront-shaping techniques

albeit at limited fields of view (FOVs). Inspired by astronomical imaging

conjugate wavefront shaping can lead to an increased field of view in microscopy

but this correction is limited to a set depth and cannot be dynamically adapted. Here

we present a conjugate wavefront-shaping scheme based on focus scanning holographic aberration probing (F-SHARP). We combine it with a compact implementation that can be readily adapted to a variety of commercial and home-built two-photon microscopes. We demonstrate the power of the method by imaging with high resolution over extended FOV (> 80 µm) deeper than 400 μm inside a mouse brain through a thinned skull.

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references

Ntziachristos, V. Going deeper than microscopy: the optical imaging frontier in biology.Nat. Methods7, 603–614 (2010)..

Denk, W., Strickler, J. H.&Webb, W. W. Two-photon laser scanning fluorescence microscopy.Science248, 73–76 (1990)..

Horton, N. G. et al. In vivo three-photon microscopy of subcortical structures within an intact mouse brain.Nat. Photonics7, 205–209 (2013)..

Ouzounov, D. G. et al. In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain.Nat. Methods14, 388–390 (2017)..

Mahajan, V. N.Optical Imaging and Aberrations: Part I. Ray Geometrical Optics(SPIE Press, Bellingham, WA, 1998).

Goodman, J. W.Statistical Optics(Wiley, New York, 2000).

Vellekoop, I. M.&Mosk, A. P. Focusing coherent light through opaque strongly scattering media.Opt. Lett.32, 2309–2311 (2007)..

Yaqoob, Z. et al. Optical phase conjugation for turbidity suppression in biological samples.Nat. Photonics2, 110–115 (2008)..

Hsieh, C. L. et al. Digital phase conjugation of second harmonic radiation emitted by nanoparticles in turbid media.Opt. Express18, 12283–12290 (2010)..

Cui, M.&Yang, C. Implementation of a digital optical phase conjugation system and its application to study the robustness of turbidity suppression by phase conjugation.Opt. Express18, 3444–3455 (2010)..

Popoff, S. M. et al. Measuring the transmission matrix in optics: an approach to the study and control of light propagation in disordered media.Phys. Rev. Lett.104, 100601 (2010)..

Booth, M. J. et al. Adaptiveaberration correction in a confocal microscope.Proc. Natl. Acad. Sci. USA99, 5788–5792 (2002)..

Neil, M. A. A. et al. Adaptive aberration correction in a two-photon microscope.J. Microsc.200, 105–108 (2000)..

Olivier, N., Débarre, D.&Beaurepaire, E. Dynamic aberration correction for multiharmonic microscopy.Opt. Lett.34, 3145–3147 (2009)..

Tao, X. D. et al. Adaptive optics confocal microscopy using direct wavefront sensing.Opt. Lett.36, 1062–1064 (2011)..

Wang, K. et al. Rapid adaptive optical recovery of optimal resolution over large volumes.Nat. Methods11, 625–628 (2014)..

Wang, K. et al. Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue.Nat. Commun.6, 7276 (2015)..

Ji, N., Milkie, D. E.&Betzig, E. Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues.Nat. Methods7, 141–147 (2010)..

Wang, C. et al. Multiplexed aberration measurement for deep tissue imaging in vivo.Nat. Methods11, 1037–1040 (2014)..

Tang, J. Y., Germain, R. N.&Cui, M. Superpenetration optical microscopy by iterative multiphoton adaptive compensation technique.Proc. Natl. Acad. Sci. USA109, 8434–8439 (2012)..

Katz, O. et al. Noninvasive nonlinear focusing and imaging through strongly scattering turbid layers.Optica1, 170–174 (2014)..

Papadopoulos, I. N. et al. Scattering compensation by focus scanning holographic aberration probing (F-SHARP).Nat. Photonics11, 116–123 (2017)..

Freund, I., Rosenbluh, M.&Feng, S. C. Memory effects in propagation of optical waves through disordered media.Phys. Rev. Lett.61, 2328–2331 (1988)..

Feng, S. C. et al. Correlations and fluctuations of coherent wave transmission through disordered media.Phys. Rev. Lett.61, 834–837 (1988)..

Schott, S. et al. Characterization of the angular memory effect of scattered light in biological tissues.Opt. Express23, 13505–13516 (2015)..

Judkewitz, B. et al. Translation correlations in anisotropically scattering media.Nat. Phys.11, 684–689 (2015)..

Osnabrugge, G. et al. Generalized optical memory effect.Optica4, 886–892 (2017)..

Hsieh, C. L. et al. Imaging through turbid layers by scanning the phase conjugated second harmonic radiation from a nanoparticle.Opt. Express18, 20723–20731 (2010)..

Vellekoop, I. M.&Aegerter, C. M. Scattered light fluorescence microscopy: imaging through turbid layers.Opt. Lett.35, 1245–1247 (2010)..

Bertolotti, J. et al. Non-invasive imaging through opaque scattering layers.Nature491, 232–234 (2012)..

Katz, O., Small, E.&Silberberg, Y. Looking around corners and through thin turbid layers in real time with scattered incoherent light.Nat. Photonics6, 549–553 (2012)..

Beckers, J. M. Increasing the size of the isoplanatic patch with multiconjugate adaptive optics.Proceedings of European Southern Observatory Conference and Workshop(EuropeanSouthern Observatory, Garching, 1988).

Johnston, D. C.&Welsh, B. M. Analysis of multiconjugate adaptive optics.J. Optical Soc. Am. A11, 394–408 (1994)..

Ragazzoni, R., Marchetti, E.&Valente, G. Adaptive-optics corrections available for the whole sky.Nature403, 54–56 (2000)..

Tokovinin, A., Le Louarn, M.&Sarazin, M. Isoplanatism in a multiconjugate adaptive optics system.J. Optical Soc. Am. A17, 1819–1827 (2000)..

Mertz, J., Paudel, H.&Bifano, T. G. Field of view advantage of conjugate adaptive optics in microscopy applications.Appl. Opt.54, 3498–3506 (2015)..

Park, J. H., Sun, W.&Cui, M. High-resolution in vivo imaging of mouse brain through the intact skull.Proc. Natl Acad. Sci. USA112, 9236–9241 (2015)..

Tao, X. D. et al. Three-dimensional focusing through scattering media using conjugate adaptive optics with remote focusing (CAORF).Opt. Express25, 10368–10383 (2017)..

Goodman, J. W.Introduction to Fourier Optics3rd edn (Roberts and Company Publishers, Greenwoood Village, 2005).

Feng, G. P. et al. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP.Neuron28, 41–51 (2000)..

Paudel, H. P. et al. Axial range of conjugate adaptive optics in two-photon microscopy.Opt. Express23, 20849–20857 (2015)..

Drew, P. J. et al. Chronic optical access through a polished and reinforced thinned skull.Nat. Methods7, 981–984 (2010)..

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