Multifocal photoacoustic microscopy using a single-element ultrasonic transducer through an ergodic relay

Yang Li; Terence T. W. Wong; Junhui Shi; Hsun-Chia Hsu; Lihong V. Wang

doi:10.1038/s41377-020-00372-x

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Multifocal photoacoustic microscopy using a single-element ultrasonic transducer through an ergodic relay

Letters

- Multifocal photoacoustic microscopy using a single-element ultrasonic transducer through an ergodic relay
- Light: Science & Applications Vol. 9, Issue 5, Pages: 1299-1305(2020)
- Affiliations：
  
  1.Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
  2.Department of Biomedical Engineering, Washington University in St. Louis, Campus Box 1097, One Brookings Drive, St. Louis, MO 63130-4899, USA
  3.Present address: Translational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering, Hong Kong University of Science and Technology, Hong Kong, China
- Author bio：
  
  Lihong V. Wang (LVW@caltech.edu)
- Funds：
- DOI：10.1038/s41377-020-00372-x
  CLC：
- Published：2020，
  
  Published Online：31 July 2020，
  
  Received：01 March 2020，
  
  Revised：17 July 2020，
  
  Accepted：20 July 2020
- Accepted：
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Li, Y. et al. Multifocal photoacoustic microscopy using a single-element ultrasonic transducer through an ergodic relay. Light: Science & Applications, 9, 1299-1305 (2020).
DOI：

Li, Y. et al. Multifocal photoacoustic microscopy using a single-element ultrasonic transducer through an ergodic relay. Light: Science & Applications, 9, 1299-1305 (2020). DOI： 10.1038/s41377-020-00372-x.

摘要

Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) has demonstrated high-spatial-resolution imaging of optical absorption in biological tissue. To date

most OR-PAM systems rely on mechanical scanning with confocally aligned optical excitation and ultrasonic detection

limiting the wide-field imaging speed of these systems. Although several multifocal OR-PA (MFOR-PA) systems have attempted to address this limitation

they are hindered by the complex design in a constrained physical space. Here

we present a two-dimensional (2D) MFOR-PAM system that utilizes a 2D microlens array and an acoustic ergodic relay. Using a single-element ultrasonic transducer

this system can detect PA signals generated from 400 optical foci in parallel and then raster scan the optical foci patterns to form an MFOR-PAM image. This system improves the imaging resolution of an acoustic ergodic relay system from 220 to 13 μm and enables 400-folds shorter scanning time than that of a conventional OR-PAM system at the same resolution and laser repetition rate. We demonstrated the imaging ability of the system with both in vitro and in vivo experiments.

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references

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