Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids

Jules Scholler; Kassandra Groux; Olivier Goureau; José-Alain Sahel; Mathias Fink; Sacha Reichman; Claude Boccara; Kate Grieve

doi:10.1038/s41377-020-00375-8

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Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids

Letters

- Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids
- Light: Science & Applications Vol. 9, Issue 5, Pages: 1306-1314(2020)
- Affiliations：
  
  1.Institut Langevin, ESPCI Paris, PSL University, CNRS, 10 rue Vauquelin, Paris, France
  2.Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France
  3.Quinze-Vingts National Eye Hospital, 28 Rue de Charenton, Paris 75012, France
  4.Fondation Ophtalmologique Rothschild, F-75019 Paris, France
  5.Department of Ophthalmology, The University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, United States
- Author bio：
  
  Kate Grieve (kategrieve@gmail.com)
- Funds：
- DOI：10.1038/s41377-020-00375-8
  CLC：
- Published：2020，
  
  Published Online：17 August 2020，
  
  Received：09 December 2019，
  
  Revised：19 June 2020，
  
  Accepted：27 July 2020
- Accepted：
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Scholler, J. et al. Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids. Light: Science & Applications, 9, 1306-1314 (2020).
DOI：

Scholler, J. et al. Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids. Light: Science & Applications, 9, 1306-1314 (2020). DOI： 10.1038/s41377-020-00375-8.

摘要

Abstract

Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids. Coloured images with an endogenous contrast linked to organelle motility are generated

with submicrometre spatial resolution and millisecond temporal resolution

creating a way to identify specific cell types in living tissue via their function.

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references

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