Metastatic status of sentinel lymph nodes in breast cancer determined with photoacoustic microscopy via dual-targeting nanoparticles

Yanfeng Dai; Xiang Yu; Jianshuang Wei; Fanxin Zeng; Yiran Li; Xiaoquan Yang; Qingming Luo; Zhihong Zhang

doi:10.1038/s41377-020-00399-0

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Metastatic status of sentinel lymph nodes in breast cancer determined with photoacoustic microscopy via dual-targeting nanoparticles

Original Articles

- Metastatic status of sentinel lymph nodes in breast cancer determined with photoacoustic microscopy via dual-targeting nanoparticles
- Light: Science & Applications Vol. 9, Issue 5, Pages: 1562-1577(2020)
- Affiliations：
  
  1.Britton Chance Center and MOE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics–Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
  2.School of Biomedical Engineering, Hainan University, Haikou, Hainan 570228, China
- Author bio：
  
  Qingming Luo (qluo@hainanu.edu.cn)
  Zhihong Zhang (czyzzh@mail.hust.edu.cn)
- Funds：
- DOI：10.1038/s41377-020-00399-0
  CLC：
- Published：2020，
  
  Published Online：16 September 2020，
  
  Received：03 May 2020，
  
  Revised：14 August 2020，
  
  Accepted：01 September 2020
- Accepted：
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Dai, Y. F. et al. Metastatic status of sentinel lymph nodes in breast cancer determined with photoacoustic microscopy via dual-targeting nanoparticles. Light: Science & Applications, 9, 1562-1577 (2019).
DOI：

Dai, Y. F. et al. Metastatic status of sentinel lymph nodes in breast cancer determined with photoacoustic microscopy via dual-targeting nanoparticles. Light: Science & Applications, 9, 1562-1577 (2019). DOI： 10.1038/s41377-020-00399-0.

摘要

Abstract

Detection of sentinel lymph nodes (SLNs) is critical to gui

de the treatment of breast cancer. However

distinguishing metastatic SLNs from normal and inflamed lymph nodes (LNs) during surgical resection remains a challenge. Here

we report a CD44 and scavenger receptor class B1 dual-targeting hyaluronic acid nanoparticle (5K-HA-HPPS) loaded with the near-infra-red fluorescent dye DiR-BOA for SLN imaging in breast cancer. The small sized (~40 nm) self-assembled 5K-HA-HPPSs accumulated rapidly in the SLNs after intradermal injection. Compared with normal popliteal LNs (N-LN)

there were ~3.2-fold and ~2.4-fold increases in fluorescence intensity in tumour metastatic SLNs (T-MLN) and inflamed LNs (Inf-LN)

respectively

6 h after nanoparticle inoculation. More importantly

photoacoustic microscopy (PAM) of 5K-HA-HPPS showed a significantly distinct distribution in T-MLN compared with N-LN and Inf-LN. Signals were mainly distributed at the centre of T-MLN but at the periphery of N-LN and Inf-LN. The ratio of PA intensity (R) at the centre of the LNs compared with that at the periphery was 5.93 ± 0.75 for T-MLNs of the 5K-HA-HPPS group

which was much higher than that for the Inf-LNs (

= 0.2 ± 0.07) and N-LNs (

= 0.45 ± 0.09). These results suggest that 5K-HA-HPPS injection combined with PAM provides a powerful tool for distinguishing metastatic SLNs from pLNs and inflamed LNs

thus guiding the removal of SLNs during breast cancer surgery.

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