New solution for fast axial scanning in fluorescence microscopy

Weijian Zong

doi:10.1038/s41377-020-00442-0

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New solution for fast axial scanning in fluorescence microscopy

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- New solution for fast axial scanning in fluorescence microscopy
- Light: Science & Applications Vol. 9, Issue 6, Pages: 1694-1695(2020)
- Affiliations：
  
  Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, Norway
- Author bio：
  
  Weijian Zong (weijian.zong@ntnu.no)
- Funds：
- DOI：10.1038/s41377-020-00442-0
  CLC：
- Published：2020，
  
  Published Online：14 December 2020，
- Accepted：
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Zong, W. J. New solution for fast axial scanning in fluorescence microscopy. Light: Science & Applications, 9, 1694-1695 (2020).
DOI：

Zong, W. J. New solution for fast axial scanning in fluorescence microscopy. Light: Science & Applications, 9, 1694-1695 (2020). DOI： 10.1038/s41377-020-00442-0.

摘要

Abstract

A novel technique based on the remote-focusing concept

using a galvanometer scanner combined with a self-fabricated "step mirror" or "tilted mirror" to transform fast lateral scanning into axial scanning

was reported as a new solution for fast

subcellular

3D fluorescence imaging.

关键词

Keywords

references

Liu, T. L. et al. Observing the cell in its native state: imaging subcellular dynamics in multicellular organisms.Science360, eaaq1392 (2018)..

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Botcherby, E. J. et al. Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates.Proc. Natl Acad. Sci. USA109, 2919-2924 (2012)..

Wu, J. L. et al. Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo.Nat. Methods17, 287-290 (2020)..

Beaulieu, D. R. et al. Simultaneous multiplane imaging with reverberation two-photon microscopy.Nat. Methods17, 283-286 (2020)..

Weisenburger, S.et al. Volumetric Ca2+imaging in the mouse brain using hybrid multiplexed sculpted light microscopy.Cell177, 1050-1066.E14 (2019)..

Chakraborty, T. et al. Converting lateral scanning into axial focusing to speed up three-dimensional microscopy.Light.: Sci. Appl.9, 165 (2020)..

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