High-resolution impedance mapping using electrically activated quantitative phase imaging

Cristina Polonschii; Mihaela Gheorghiu; Sorin David; Szilveszter Gáspár; Sorin Melinte; Hassaan Majeed; Mikhail E. Kandel; Gabriel Popescu; Eugen Gheorghiu

doi:10.1038/s41377-020-00461-x

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High-resolution impedance mapping using electrically activated quantitative phase imaging

Articles

- High-resolution impedance mapping using electrically activated quantitative phase imaging
- Light: Science & Applications Vol. 10, Issue 2, Pages: 186-196(2021)
- Affiliations：
  
  1.International Centre of Biodynamics, 060101 Bucharest, Romania
  2.Institute of Information and Communication Technologies, Electronics and Applied Mathematics, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium
  3.Quantitative Light Imaging Laboratory, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
- Author bio：
  
  Eugen Gheorghiu (gpopescu@illinois.edu)
  Gabriel Popescu (egheorghiu@biodyn.ro)
- Funds：
- DOI：10.1038/s41377-020-00461-x
  CLC：
- Published：2021，
  
  Published Online：21 January 2021，
  
  Received：14 July 2020，
  
  Revised：16 December 2020，
  
  Accepted：29 December 2020
- Accepted：
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Polonschii, C. et al. High-resolution impedance mapping using electrically activated quantitative phase imaging. Light: Science & Applications, 10, 186-196 (2021).
DOI：

Polonschii, C. et al. High-resolution impedance mapping using electrically activated quantitative phase imaging. Light: Science & Applications, 10, 186-196 (2021). DOI： 10.1038/s41377-020-00461-x.

摘要

Abstract

Retrieving electrical impedance maps at the nanoscale rapidly via nondestructive inspection with a high signal-to-noise ratio is an unmet need

likely to impact various applications from biomedicine to energy conversion. In this study

we develop a multimodal functional imaging instrument that is characterized by the dual capability of impedance mapping and phase quantitation

high spatial resolution

and low temporal noise. To achieve this

we advance a quantitative phase imaging system

referred to as epi-magnified image spatial spectrum microscopy combined with electrical actuation

to provide complementary maps of the optical path and electrical impedance. We demonstrate our system with high-resolution maps of optical path differences and electrical impedance variations that can distinguish nanosized

semi-transparent

structured coatings involving two materials with relatively similar electrical properties. We map heterogeneous interfaces corresponding to an indium tin oxide layer exposed by holes with diameters as small as ~550 nm in a titanium (dioxide) over-layer deposited on a glass support. We show that electrical modulation during the phase imaging of a macro-electrode is decisive for retrieving electrical impedance distributions with submicron spatial resolution and beyond the limitations of electrode-based technologies (surface or scanning technologies). The findings

which are substantiated by a theoretical model that fits the experimental data very well enable achieving electro-optical maps with high spatial and temporal resolutions. The virtues and limitations of the novel optoelectrochemical method that provides grounds for a wider range of electrically modulated optical methods for measuring the electric field locally are critically discussed.

关键词

Keywords

references

Gan, Q., Bartoli, F. J.&Kafafi, Z. H. Plasmonic-enhanced organic photovoltaics: breaking the 10% efficiency barrier.Adv. Mater.25, 2385-2396 (2013)..

Kuehnel, M. F. et al. ZnSe nanorods as visible-light absorbers for photocatalytic and photoelectrochemical H2evolution in.Water Angew. Chim. Int. Ed.58, 5059-5063 (2019)..

Dias, P. et al. Transparent cuprous oxide photocathode enabling a stacked tandem cell for unbiased water splitting.Adv. Energy Mater.5, 1501537 (2015)..

Branch, S. D. et al. Optically transparent thin-film electrode chip for spectroelectrochemical sensing.Anal. Chem.89, 7324-7332 (2017)..

Spira, M. E.&Hai, A. Multi-electrode array technologies for neuroscience and cardiology.Nat. Nanotechnol.8, 83-94 (2013)..

Lemineur, J. F. et al. In situ optical monitoring of the electrochemical conversion of dielectric nanoparticles: from multi-step charge injection to nanoparticle motion.J. Am. Chem. Soc.142, 7937-7946 (2020)..

Zhu, H. et al. Detection of molecules and charges with a bright field optical microscope.Anal. Chem.92, 5904-5909 (2020)..

Bandarenka, A. S. et al. Localized electrochemical impedance spectroscopy: visualization of spatial distributions of the key parameters describing solid/liquid interfaces.Anal. Chem.85, 2443-2448 (2013)..

Polcari, D., Dauphin-Ducharme, P.&Mauzeroll, J. Scanning electrochemical microscopy: a comprehensive review of experimental parameters from 1989 to 2015.Chem. Rev.116, 13234-13278 (2016)..

Stephens, L. I.&Mauzeroll, J. Altered spatial resolution of scanning electrochemical microscopy induced by multifunctional dual-barrel microelectrodes.Anal. Chem.90, 6796-6803 (2018)..

Koley, G., Spencer, M. G.&Bhangale, H. R. Cantilever effects on the measurement of electrostatic potentials by scanning Kelvin probe microscopy.Appl. Phys. Lett.79, 545-547 (2001)..

Domanski, A. L. et al. Kelvin probe force microscopy in nonpolar liquids.Langmuir28, 13892-13899 (2012)..

Foley, K. J., Shan, X. N.&Tao,N. J. Surface impedance imaging technique.Anal. Chem.80, 5146-5151 (2008)..

Liu, X. W. et al. Plasmonic-based electrochemical impedance imaging of electrical activities in single cells.Angew. Chim. Int. Ed.56, 8855-8859 (2017)..

Wang, W. et al. Single cells and intracellular processes studied by a plasmonic-based electrochemical impedance microscopy.Nat. Chem.3, 249-255 (2011)..

Liang, W. B. et al. Measurement of small molecule binding kinetics on a protein microarray by plasmonic-based electrochemical impedance imaging.Anal. Chem.86, 9860-9865 (2014)..

MacGriff, C. et al. Charge-based detection of small molecules by plasmonic-based electrochemical impedance microscopy.Anal. Chem.85, 6682-6687 (2013)..

Shan, X. N. et al. Imaging local electrochemical current via surface plasmon resonance.Science327, 1363-1366 (2010)..

Popescu, G.Quantitative Phase Imaging of Cells and Tissues. (McGraw-Hill, New York, 2011).

Kandel, M. E. et al. Phase imaging with computational specificity (PICS) for measuring dry mass changes in sub-cellular compartments.Nat. Commun.11, 6256 (2020)..

Langehanenberg, P., von Bally, G.&Kemper, B. Application of partially coherent light in live cell imaging with digital holographic microscopy.J. Mod. Opt.57, 709-717 (2010)..

Merola, F. et al. Tomographic flow cytometry by digital holography.Light Sci. Appl.6, e16241 (2017)..

Miccio, L. et al. Red blood cell as an adaptive optofluidic microlens.Nat. Commun.6, 6502 (2015)..

Park, Y. K., Depeursinge, C.&Popescu, G. Quantitative phase imaging in biomedicine.Nat. Photonics12, 578-589 (2018)..

Ash, W. M. III, Krzewina, L.&Kim, M. K. Quantitative imaging of cellular adhesion by total internal reflection holographic microscopy.Appl. Opt.48, H144-H152 (2009)..

Mandracchia, B. et al. Label-free quantification of the effects of lithium niobate polarization on cell adhesion via holographic microscopy.J. Biophotonics11, e201700332 (2018)..

Merola, F. et al. Phase contrast tomography at lab on chip scale by digital holography.Methods136, 108-115 (2018)..

Charrière, F. et al. Cell refractive index tomography by digital holographic microscopy.Opt. Lett.31, 178-180 (2006)..

Lee, M. et al. Label-free optical quantification of structural alterations in Alzheimer's disease.Sci. Rep.6, 31034 (2016)..

Shan, M. G. et al. White-light diffraction phase microscopy at doubled space-bandwidth product.Opt. Express24, 29033-29039 (2016)..

Wang, Z. et al. Spatial light interference microscopy (SLIM).Opt. Express19, 1016-1026 (2011)..

Kandel, M. E. et al. Epi-illumination gradient light interference microscopy for imaging opaque structures.Nat. Commun.10, 4691 (2019)..

Nguyen, T. H. et al. Gradient light interference microscopy for 3D imaging of unlabeled specimens.Nat. Commun.8, 210 (2017)..

Majeed, H. et al. Magnified image spatial spectrum (MISS) microscopy for nanometer and millisecond scale label-free imaging.Opt. Express26, 5423-5440 (2018)..

Munteanu, R. E. et al. High spatial resolution electrochemical biosensing using reflected light microscopy.Sci. Rep.9, 15196 (2019)..

Bhaduri, B. et al. Diffraction phase microscopy: principles and applications in materials and life sciences.Adv. Opt. Photonics6, 57-119 (2014)..

Shan, X. N. et al. Plasmonic-based imaging of local square wave voltammetry.Anal. Chem.83, 7394-7399 (2011)..

Wang, S. P. et al. Electrochemical surface plasmon resonance: basic formalism and experimental validation.Anal. Chem.82, 935-941 (2010)..

Yuan, L., Tao, N. J.&Wang, W. Plasmonic imaging of electrochemical impedance.Annu. Rev. Anal. Chem.10, 183-200 (2017)..

Zhou, X. L. et al. Surface plasmon resonance microscopy: from single-molecule sensing to single-cell imaging.Angew. Chim. Int. Ed.59, 1776-1785 (2020)..

Rosu-Hamzescu, M. et al. High speed CMOS acquisition system based on FPGA embedded image processing for electro-optical measurements.Rev. Sci. Instrum.89, 065103 (2018)..

Beekers, I. et al. Combined confocal microscope and brandaris 128 ultra-high-speed camera.Ultrasound Med. Biol.45, 2575-2582 (2019)..

Lioubimov, V. et al. Effect of varying electric potential on surface-plasmon resonance sensing.Appl. Opt.43, 3426-3432 (2004)..

Pleskov, Y. V. Electric double layer on semiconductor electrodes. inComprehensive Treatise of Electrochemistry(eds Bockris, J. O. M., Conway, B. E.,&Yeager, E. ) 291-328 (Springer, Boston, 1980).

Brett, C. M. A.&Brett, A. M. O.Electrochemistry—Principles, methods and applications. (Oxford University Press, Oxford, 1993).

Almog, I. F., Bradley, M. S.&Bulović, V.The Lorentz Oscillator and its Applications. (MIT OpenCourseWare, 2011) 1-34.

Cherednichenko, K.&Graham, W. Frequency-dependent impedance and surface waves on the boundary of a stratified dielectric medium.Philos. Trans. R. Soc. A: Math. Phys. Eng. Sci.377, 20190218 (2019)..

Gharbi, T. et al. Fitting optical properties of metals by Drude-Lorentz and partial-fraction models in the [0.5;6]eV range.Optical Mater. Express10, 1129-1162 (2020)..

McIntyre, J. D. E. Electrochemical modulation spectroscopy.Surf. Sci.37, 658-682 (1973)..

Polonschii, C. et al. Complementarity of EIS and SPR to reveal specific and nonspecific binding when interrogating a model bioaffinity sensor; perspective offered by plasmonic based EIS.Anal. Chem.86, 8553-8562 (2014)..

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