Spatial heterogeneity of oxygenation and haemodynamics in breast cancer resolved in vivo by conical multispectral optoacoustic mesoscopy

Jiao Li; Andrei Chekkoury; Jaya Prakash; Sarah Glasl; Paul Vetschera; Benno Koberstein-Schwarz; Ivan Olefir; Vipul Gujrati; Murad Omar; Vasilis Ntziachristos

doi:10.1038/s41377-020-0295-y

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Spatial heterogeneity of oxygenation and haemodynamics in breast cancer resolved in vivo by conical multispectral optoacoustic mesoscopy

Original Articles

- Spatial heterogeneity of oxygenation and haemodynamics in breast cancer resolved in vivo by conical multispectral optoacoustic mesoscopy
- Light: Science & Applications Vol. 9, Issue 3, Pages: 595-609(2020)
- Affiliations：
  
  1.School of Precision Instruments and Optoelectronics Engineering, Tianjin University, No.92, Weijin Road, Nankai District, 300072 Tianjin, China
  2.Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany
  3.Chair of Biological Imaging, TranslaTUM, Technische Universität München, Ismaningerstr. 22, D-81675 Munich, Germany
  4.Department of Instrumentation and Applied Physics, Indian Institute of Science Bangalore, CV Raman Rd, Bengaluru 560012 Karnataka, India
- Author bio：
  
  Vasilis Ntziachristos (v.ntziachristos@tum.de)
- Funds：
- DOI：10.1038/s41377-020-0295-y
  CLC：
- Published：2020，
  
  Published Online：13 April 2020，
  
  Received：28 August 2019，
  
  Revised：10 February 2020，
  
  Accepted：19 March 2020
- Accepted：
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Li, J. et al. Spatial heterogeneity of oxygenation and haemodynamics in breast cancer resolved in vivo by conical multispectral optoacoustic mesoscopy. Light: Science & Applications, 9, 595-609 (2020).
DOI：

Li, J. et al. Spatial heterogeneity of oxygenation and haemodynamics in breast cancer resolved in vivo by conical multispectral optoacoustic mesoscopy. Light: Science & Applications, 9, 595-609 (2020). DOI： 10.1038/s41377-020-0295-y.

摘要

Abstract

The characteristics of tumour development and metastasis relate not only to genomic heterogeneity but also to spatial heterogeneity

associated with variations in the intratumoural arrangement of cell populations

vascular morphology and oxygen and nutrient supply. While optical (photonic) microscopy is commonly employed to visualize the tumour microenvironment

it assesses only a few hundred cubic microns of tissue. Therefore

it is not suitable for investigating biological processes at the level of the entire tumour

which can be at least four orders of magnitude larger. In this study

we aimed to extend optical visualization and resolve spatial heterogeneity throughout the entire tumour volume. We developed an optoacoustic (photoacoustic) mesoscope adapted to solid tumour imaging and

in a pilot study

offer the first insights into cancer optical contrast heterogeneity in vivo at an unprecedented resolution of < 50 μm throughout the entire tumour mass. Using spectral methods

we resolve unknown patterns of oxygenation

vasculature and perfusion in three types of breast cancer and showcase different levels of structural and functional organization. To our knowledge

these results are the most detailed insights of optical signatures reported throughout entire tumours in vivo

and they position optoacoustic mesoscopy as a unique investigational tool linking microscopic and macroscopic observations.

关键词

Keywords

references

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