Endo-microscopy beyond the Abbe and Nyquist limits

Lyubov V. Amitonova; Johannes F. de Boer

doi:10.1038/s41377-020-0308-x

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Endo-microscopy beyond the Abbe and Nyquist limits

Original Articles

- Endo-microscopy beyond the Abbe and Nyquist limits
- Light: Science & Applications Vol. 9, Issue 3, Pages: 783-794(2020)
- Affiliations：
  
  1.LaserLaB, Department of Physics and Astronomy, Vrije Universiteit Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
  2.Advanced Research Center for Nanolithography (ARCNL), Science Park 106, 1098 XG Amsterdam, The Netherlands
- Author bio：
  
  Lyubov V. Amitonova (l.amitonova@vu.nl)
- Funds：
- DOI：10.1038/s41377-020-0308-x
  CLC：
- Published：2020，
  
  Published Online：07 May 2020，
  
  Received：25 September 2019，
  
  Revised：01 April 2020，
  
  Accepted：03 April 2020
- Accepted：
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Amitonova, L. V. & de Boer, J. F. et al. Endo-microscopy beyond the Abbe and Nyquist limits. Light: Science & Applications, 9, 783-794 (2020).
DOI：

Amitonova, L. V. & de Boer, J. F. et al. Endo-microscopy beyond the Abbe and Nyquist limits. Light: Science & Applications, 9, 783-794 (2020). DOI： 10.1038/s41377-020-0308-x.

摘要

Abstract

For several centuries

far-field optical microscopy has remained a key instrument in many scientific disciplines

including physical

chemical

and biomedical research. Nonetheless

far-field imaging has many limitations: the spatial resolution is controlled by the diffraction of light

and the imaging speed follows the Nyquist–Shannon sampling theorem. The recent development of super-resolution techniques has pushed the limits of spatial resolution. However

these methods typically require complicated setups and long acquisition times and are still not applicable to deep-tissue bioimaging. Here

we report imaging through an ultra-thin fibre probe with a spatial resolution beyond the Abbe limit and a temporal resolution beyond the Nyquist limit simultaneously in a simple and compact setup. We use the random nature of mode coupling in a multimode fibre

the sparsity constraint and compressive sensing reconstruction. The new approach of super-resolution endo-microscopy does not use any specific properties of the fluorescent label

such as depletion or stochastic activation of the molecular fluorescent state

and therefore can be used for label-free imaging. We demonstrate a spatial resolution more than 2 times better than the diffraction limit and an imaging speed 20 times faster than the Nyquist limit. The proposed approach can significantly expand the realm of the application of nanoscopy for bioimaging.

关键词

Keywords

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