Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo

Zhongya Qin; Sicong He; Chao Yang; Jasmine Sum-Yee Yung; Congping Chen; Christopher Kai-Shun Leung; Kai Liu; Jianan Y. Qu

doi:10.1038/s41377-020-0317-9

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Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo

Original Articles

- Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo
- Light: Science & Applications Vol. 9, Issue 3, Pages: 764-774(2020)
- Affiliations：
  
  1.Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
  2.Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
  3.Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China
  4.State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
  5.Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
- Author bio：
  
  Jianan Y. Qu (eequ@ust.hk)
- Funds：
- DOI：10.1038/s41377-020-0317-9
  CLC：
- Published：2020，
  
  Published Online：06 May 2020，
  
  Received：06 February 2020，
  
  Revised：11 April 2020，
  
  Accepted：15 April 2020
- Accepted：
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Qin, Z. Y. et al. Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo. Light: Science & Applications, 9, 764-774 (2020).
DOI：

Qin, Z. Y. et al. Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo. Light: Science & Applications, 9, 764-774 (2020). DOI： 10.1038/s41377-020-0317-9.

摘要

Abstract

In vivo fundus imaging offers non-invasive access to neuron structures and biochemical processes in the retina. However

optical aberrations of the eye degrade the imaging resolution and prevent visualization of subcellular retinal structures. We developed an adaptive optics two-photon excitation fluorescence microscopy (AO-TPEFM) system to correct ocular aberrations based on a nonlinear fluorescent guide star and achieved subcellular resolution for in vivo fluorescence imaging of the mouse retina. With accurate wavefront sensing and rapid aberration correction

AO-TPEFM permits structural and functional imaging of the mouse retina with submicron resolution. Specifically

simultaneous functional calcium imaging of neuronal somas and dendrites was demonstrated. Moreover

the time-lapse morphological alteration and dynamics of microglia were characterized in a mouse model of retinal disorder. In addition

precise laser axotomy was achieved

and degeneration of retinal nerve fibres was studied. This high-resolution AO-TPEFM is a promising tool for non-invasive retinal imaging and can facilitate the understanding of a variety of eye diseases as well as neurodegenerative disorders in the central nervous system.

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references

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