Laser particles with omnidirectional emission for cell tracking

Shui-Jing Tang; Paul H. Dannenberg; Andreas C. Liapis; Nicola Martino; Yue Zhuo; Yun-Feng Xiao; Seok-Hyun Yun

doi:10.1038/s41377-021-00466-0

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Laser particles with omnidirectional emission for cell tracking

Articles

- Laser particles with omnidirectional emission for cell tracking
- Light: Science & Applications Vol. 10, Issue 2, Pages: 197-207(2021)
- Affiliations：
  
  1.Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA
  2.State Key Laboratory for Mesoscopic Physics and Frontiers Science Center for Nano-optoelectronics, School of Physics, Peking University, 100871 Beijing, China
  3.Harvard-MIT Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA
- Author bio：
  
  Yun-Feng Xiao yfxiao@pku.edu.cn
  SeokHyun Yun (syun@hms.harvard.edu)
- Funds：
- DOI：10.1038/s41377-021-00466-0
  CLC：
- Published：2021，
  
  Published Online：25 January 2021，
  
  Received：13 August 2020，
  
  Revised：05 January 2021，
  
  Accepted：06 January 2021
- Accepted：
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Tang, S. J. et al. Laser particles with omnidirectional emission for cell tracking. Light: Science & Applications, 10, 197-207 (2021).
DOI：

Tang, S. J. et al. Laser particles with omnidirectional emission for cell tracking. Light: Science & Applications, 10, 197-207 (2021). DOI： 10.1038/s41377-021-00466-0.

摘要

Abstract

The ability to track individual cells in space over time is crucial to analyzing heterogeneous cell populations. Recently

microlaser particles have emerged as unique optical probes for massively multiplexed single-cell tagging. However

the microlaser far-field emission is inherently direction-dependent

which causes strong intensity fluctuations when the orientation of the particle varies randomly inside cells. Here

we demonstrate a general solution based on the incorporation of nanoscale light scatterers into microlasers. Two schemes are developed by introducing either boundary defects or a scattering layer into microdisk lasers. The resulting laser output is omnidirectional

with the minimum-to-maximum ratio of the angle-dependent intensity improving from 0.007 (−24 dB) to > 0.23 (−6 dB). After transfer into live cells in vitro

the omnidirectional laser particles within moving cells could be tracked continuously with high signal-to-noise ratios for 2 h

while conventional microlasers exhibited frequent signal loss causing tracking failure.

关键词

Keywords

references

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