
Optics and Photonics Group, Faculty of Engineering, University of Nottingham, University Park, Nottingham NG7 2RD, UK
Salvatore La Cavera III (salvatore.lacaveraiii@nottingham.ac.uk)
Published:30 June 2021,
Published Online:27 April 2021,
Received:03 October 2020,
Revised:30 March 2021,
Accepted:07 April 2021
Scan QR Code
Cavera III, S. L. et al. Phonon imaging in 3D with a fibre probe. Light: Science & Applications, 10, 1007-1019 (2021).
Cavera III, S. L. et al. Phonon imaging in 3D with a fibre probe. Light: Science & Applications, 10, 1007-1019 (2021). DOI: 10.1038/s41377-021-00532-7.
We show for the first time that a single ultrasonic imaging fibre is capable of simultaneously accessing 3D spatial information and mechanical properties from microscopic objects. The novel measurement system consists of two ultrafast lasers that excite and detect high-frequency ultrasound from a nano-transducer that was fabricated onto the tip of a single-mode optical fibre. A signal processing technique was also developed to extract nanometric in-depth spatial measurements from GHz frequency acoustic waves
while still allowing Brillouin spectroscopy in the frequency domain. Label-free and non-contact imaging performance was demonstrated on various polymer microstructures. This singular device is equipped with optical lateral resolution
2.5 μm
and a depth-profiling precision of 45 nm provided by acoustics. The endoscopic potential for this device is exhibited by extrapolating the single fibre to tens of thousands of fibres in an imaging bundle. Such a device catalyses future phonon endomicroscopy technology that brings the prospect of label-free in vivo histology within reach.
Polglase, A. L. et al. A fluorescence confocal endomicroscope for in vivo microscopy of the upper- and the lower-GI tract.Gastrointest. Endosc.62, 686–695 (2005)..
Flusberg, B. A. et al. Fiber-optic fluorescence imaging.Nat. Methods2, 941–950 (2005)..
Oh, G., Chung, E.&Yun, S. H. Optical fibers for high-resolution in vivo microendoscopic fluorescence imaging.Opt. Fiber Technol.19, 760–771 (2013)..
Yelin, D. et al. Three-dimensional miniature endoscopy.Nature443, 765 (2006)..
Kim, P. et al. In vivo wide-area cellular imaging by side-view endomicroscopy.Nat. Methods7, 303–305 (2010)..
Goetz, M., Malek, N. P.&Kiesslich, R. Microscopic imaging in endoscopy: endomicroscopy and endocytoscopy.Nat. Rev. Gastroenterol. Hepatol.11, 11–18 (2014)..
Pahlevaninezhad, H. et al. Nano-optic endoscope for high-resolution optical coherence tomography in vivo.Nat. Photonics12, 540–547 (2018)..
Plöschner, M., Tyc, T.&Čižmár, T. Seeing through chaos in multimode fibres.Nat. Photonics9, 529–535 (2015)..
Leite, I. T. et al. Three-dimensional holographic optical manipulation through a high-numerical-aperture soft-glass multimode fibre.Nat. Photonics12, 33–39 (2018)..
Choi, Y. et al. Scanner-free and wide-field endoscopic imaging by using a single multimode optical fiber.Phys. Rev. Lett.109, 203901 (2012)..
Orth, A. et al. Optical fiber bundles: ultra-slim light field imaging probes.Sci. Adv.5, eaav1555 (2019)..
Amitonova, L. V.&de Boer, J. F. Endo-microscopy beyond the Abbe and Nyquist limits.Light. Sci. Appl.9, 81 (2020)..
Vasquez-Lopez, S. A. et al. Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber.Light. Sci. Appl.7, 110 (2018)..
Scarcelli, G.&Yun, S. H. Confocal Brillouin microscopy for three-dimensional mechanical imaging.Nat. Photonics2, 39–43 (2008)..
Scarcelli, G. et al. Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy.Nat. Methods12, 1132–1134 (2015)..
Dehoux, T. et al. All-optical broadband ultrasonography of single cells.Sci. Rep.5, 8650 (2015)..
Viel, A. et al. Picosecond ultrasounds as elasticity probes in neuron-like cells models.Appl. Phys. Lett.115, 213701 (2019)..
Pérez-Cota, F. et al. New insights into the mechanical properties ofAcanthamoeba castellaniicysts as revealed by phonon microscopy.Biomed. Opt. Express10, 2399–2408 (2019)..
Liu, L. W. et al. Label-free multi-parametric imaging of single cells: dual picosecond optoacoustic microscopy.J. Biophotonics12, e201900045 (2019)..
Lemons, R. A.&Quate, C. F. Acoustic microscope—scanning version.Appl. Phys. Lett.24, 163–165 (1974)..
Colchester, R. J. et al. Broadband miniature optical ultrasound probe for high resolution vascular tissue imaging.Biomed. Opt. Express6, 1502–1511 (2015)..
Strohm, E. M., Moore, M. J.&Kolios, M. C. High resolution ultrasound and photoacoustic imaging of single cells.Photoacoustics4, 36–42 (2016)..
Strohm, E. M., Moore, M. J.&Kolios, M. C. Single cell photoacoustic microscopy: a review.IEEE J. Sel. Top. Quantum Electron.22, 6801215 (2016)..
Finlay, M. C. et al. Through-needle all-optical ultrasound imaging in vivo: a preclinical swine study.Light. Sci. Appl.6, e17103 (2017)..
Liu, L. W. et al. Remote imaging of single cell 3D morphology with ultrafast coherent phonons and their resonance harmonics.Sci. Rep.9, 6409 (2019)..
Thomsen, C. et al. Coherent phonon generation and detection by picosecond light pulses.Phys. Rev. Lett.53, 989–992 (1984)..
Brillouin, L. Diffusion de la lumière et des rayons X par un corps transparent homogène: influence de l'agitation thermique.Ann. Phys.9, 88–122 (1922)..
Pérez-Cota, F. et al. High resolution 3D imaging of living cells with sub-optical wavelength phonons.Sci. Rep.6, 39326 (2016)..
Danworaphong, S. et al. Three-dimensional imaging of biological cells with picosecond ultrasonics.Appl. Phys. Lett.106, 163701 (2015)..
Ansari, R. et al. All-optical forward-viewing photoacoustic probe for high-resolution 3D endoscopy.Light. Sci. Appl.7, 75 (2018)..
Thomsen, C. et al. Picosecond interferometric technique for study of phonons in the brillouin frequency range.Opt. Commun.60, 55–58 (1986)..
Gusev, V. E.&Ruello, P. Advances in applications of time-domain Brillouin scattering for nanoscale imaging.Appl. Phys. Rev.5, 031101 (2018)..
La Cavera, S. et al. Time resolved Brillouin fiber-spectrometer.Opt. Express27, 25064–25071 (2019)..
Pérez-Cota, F. et al. Apparent attenuation by opto-acoustic defocus in phonon microscopy.Photoacoustics19, 100180 (2020)..
Prevedel, R. et al. Brillouin microscopy: an emerging tool for mechanobiology.Nat. Methods16, 969–977 (2019)..
Devos, A.&Côte, R. Strong oscillations detected by picosecond ultrasonics in silicon: evidence for an electronic-structure effect.Phys. Rev. B70, 125208 (2004)..
Dehoux, T., Tsapis, N.&Audoin, B. Relaxation dynamics in single polymer microcapsules probed with laser-generated GHz acoustic waves.Soft Matter8, 2586–2589 (2012)..
Dehoux, T. et al. Probing single-cell mechanics with picosecond ultrasonics.Ultrasonics56, 160–171 (2015)..
Matsuda, O.&Wright, O. B. Reflection and transmission of light in multilayers perturbed by picosecond strain pulse propagation.J. Opt. Soc. Am. B19, 3028–3041 (2002)..
Uchida, N. Direct measurement of photoelastic coefficients by ultrasonic light diffraction technique.Jpn. J. Appl. Phys.8, 329–333 (1969)..
Newnham, R. E.Properties of Materials: Anisotropy, Symmetry, Structure(Oxford: Oxford University Press, 2005).
Yang, F. et al. Study of phonon propagation in water using picosecond ultrasonics.J. Phys. Conf. Ser.92, 012024 (2007)..
Smith, R. J. et al. 3D phonon microscopy with sub-micron axial-resolution.Sci. Rep.11, 3301 (2021)..
Nikitin, S. M. et al. Revealing sub-μm and μm-scaletextures in H2O ice at megabar pressures by time-domain Brillouin scattering.Sci. Rep.5, 9352 (2015)..
Guo, H. et al. Photoacoustic endoscopy: a progress review.J. Biophotonics13, e202000217 (2020)..
Jabbour, J. M. et al. Confocal endomicroscopy: instrumentation and medical applications.Ann. Biomed. Eng.40, 378–397 (2012)..
Che, S. et al. A scanning acoustic microscope based on picosecond ultrasonics.Ultrasonics56, 153–159 (2015)..
Dehoux, T. et al. Optical tracking of picosecond coherent phonon pulse focusing inside a sub-micron object.Light. Sci. Appl.5, e16082 (2016)..
Fuentes-Domínguez, R. et al. Characterising the size and shape of metallic nano-structures by their acoustic vibrations.Nanoscale12, 14230–14236 (2020)..
Harasaki, A., Schmit, J.&Wyant, J. C. Offset of coherent envelope position due to phase change on reflection.Appl. Opt.40, 2102–2106 (2001)..
O'Mahony, C. et al. Characterization of micromechanical structures using white-light interferometry.Meas. Sci. Technol.14, 1807–1814 (2003)..
Dufrêne, Y. F. et al. Imaging modes of atomic force microscopy for application in molecular and cell biology.Nat. Nanotechnol.12, 295–307 (2017)..
Noor, N. et al. 3D printing of personalized thick and perfusable cardiac patches and hearts.Adv. Sci.6, 1900344 (2019)..
Elzinga, P. A. et al. Pump/probe spectroscopy by asynchronous optical sampling.Appl. Spectrosc.41, 2–4 (1987)..
Smith, R. J. et al. Optically excited nanoscale ultrasonic transducers.J. Acoust. Soc. Am.137, 219–227 (2015)..
0
Views
0
Downloads
0
CSCD
Publicity Resources
Related Articles
Related Author
Related Institution
京公网安备11010802024621