Isotropically resolved label-free tomographic imaging based on tomographic moulds for optical trapping

Moosung Lee; Kyoohyun Kim; Jeonghun Oh; YongKeun Park

doi:10.1038/s41377-021-00535-4

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Isotropically resolved label-free tomographic imaging based on tomographic moulds for optical trapping

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- Isotropically resolved label-free tomographic imaging based on tomographic moulds for optical trapping
- Light: Science & Applications Vol. 10, Issue 6, Pages: 1061-1069(2021)
- Affiliations：
  
  1.Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, South Korea
  2.KAIST Institute for Health Science and Technology, KAIST, Daejeon 34141, South Korea
  3.Tomocube Inc., Daejeon 34109, South Korea
  4.Present address: Max Planck Institute for the Science of Light & Max-Planck-Zentrum für Physik und Medizin, 91058 Erlangen, Germany
- Author bio：
  
  YongKeun Park (yk.park@kaist.ac.kr)
- Funds：
- DOI：10.1038/s41377-021-00535-4
  CLC：
- Published：30 June 2021，
  
  Published Online：17 May 2021，
  
  Received：22 November 2020，
  
  Revised：06 April 2021，
  
  Accepted：14 April 2021
- Accepted：
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Lee, M. et al. Isotropically resolved label-free tomographic imaging based on tomographic moulds for optical trapping. Light: Science & Applications, 10, 1061-1069 (2021).
DOI：

Lee, M. et al. Isotropically resolved label-free tomographic imaging based on tomographic moulds for optical trapping. Light: Science & Applications, 10, 1061-1069 (2021). DOI： 10.1038/s41377-021-00535-4.

摘要

Abstract

A major challenge in three-dimensional (3D) microscopy is to obtain accurate spatial information while simultaneously keeping the microscopic samples in their native states. In conventional 3D microscopy

axial resolution is inferior to spatial resolution due to the inaccessibility to side scattering signals. In this study

we demonstrate the isotropic microtomography of free-floating samples by optically rotating a sample. Contrary to previous approaches using optical tweezers with multiple foci which are only applicable to simple shapes

we exploited 3D structured light traps that can stably rotate freestanding complex-shaped microscopic specimens

and side scattering information is measured at various sample orientations to achieve isotropic resolution. The proposed method yields an isotropic resolution of 230 nm and captures structural details of colloidal multimers and live red blood cells

which are inaccessible using conventional tomographic microscopy. We envision that the proposed approach can be deployed for solving diverse imaging problems that are beyond the examples shown here.

关键词

Keywords

references

Streibl, N. Three-dimensional imaging by a microscope.J. Opt. Soc. Am. A2, 121–127 (1985)..

Kim, K. et al. Optical diffraction tomography techniques for the study of cell pathophysiology.J. Biomed. Photonics Eng.2, 020201 (2016)..

Lim, J. et al. Comparative study of iterative reconstruction algorithms for missing cone problems in optical diffraction tomography.Opt. Express23, 16933–16948 (2015)..

Zhang, Y. B. et al. Wide-field imaging of birefringent synovial fluid crystals using lens-free polarized microscopy for gout diagnosis.Sci. Rep.6, 28793 (2016)..

Park, Y. et al. Refractive index maps and membrane dynamics of human red blood cells parasitized byPlasmodium falciparum.Proc. Natl Acad. Sci. USA105, 13730–13735 (2008)..

Park, Y. et al. Measurement of red blood cell mechanics during morphological changes.Proc. Natl Acad. Sci. USA107, 6731–6736 (2010)..

Oh, J. et al. Three-dimensional label-free observation of individual bacteria upon antibiotic treatment using optical diffraction tomography.Biomed. Opt. Express11, 1257–1267 (2020)..

Zhou, K. C. et al. Optical coherence refraction tomography.Nat. Photonics13, 794–802 (2019)..

Van Rooij, J.&Kalkman, J. Large-scale high-sensitivity optical diffraction tomography of zebrafish.Biomed. Opt. Express10, 1782–1793 (2019)..

Simon, B. et al. Tomographic diffractive microscopy with isotropic resolution.Optica4, 460–463 (2017)..

Kim, K., Yoon, J.&Park, Y. Large-scale optical diffraction tomography for inspection of optical plastic lenses.Opt. Lett.41, 934–937 (2016)..

Merola, F. et al. Tomographic flow cytometry by digital holography.Light: Sci. Appl.6, e16241 (2017)..

Schürmann, M. et al. Three-dimensional correlative single-cell imaging utilizing fluorescence and refractive index tomography.J. Biophotonics11, e201700145 (2018)..

Ashkin, A. et al. Observation of a single-beam gradient force optical trap for dielectric particles.Opt. Lett.11, 288–290 (1986)..

Curtis, J. E., Koss, B. A.&Grier, D. G. Dynamic holographic optical tweezers.Opt. Commun.207, 169–175 (2002)..

Kim, K., Yoon, J.&Park, Y. Simultaneous 3D visualization and position tracking of optically trapped particles using optical diffraction tomography.Optica2, 343–346 (2015)..

Habaza, M. et al. Tomographic phase microscopy with 180° rotation of live cells in suspension by holographic optical tweezers.Opt. Lett.40, 1881–1884 (2015)..

Lin, Y. C. et al. Optically driven full-angle sample rotation for tomographic imaging in digital holographic microscopy.Opt. Lett.42, 1321–1324 (2017)..

Vinoth, B. et al. Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution.Sci. Rep.8, 5943 (2018)..

Mohanty, S. K., Uppal, A.&Gupta, P. K. Self-rotation of red blood cells in optical tweezers: prospects for high throughput malaria diagnosis.Biotechnol. Lett.26, 971–974 (2004)..

Rancourt-Grenier, S. et al. Dynamic deformation of red blood cell in Dual-trap Optical Tweezers.Opt. Express18, 10462–10472 (2010)..

Kim, K.&Park, Y. Tomographic active optical trapping of arbitrarily shaped objects by exploiting 3D refractive index maps.Nat. Commun.8, 15340 (2017)..

Jackson, J. D.Classical Electrodynamics.3rd edn. (Wiley, 1999).

Reitz, J. R., Milford, F. J.&Christy, R. W.Foundations of Electromagnetic Theory.4th edn. (Addison-Wesley Publishing Company, 2008).

Park, Y., Depeursinge, C.&Popescu, G. Quantitative phase imaging in biomedicine.Nat. Photonics12, 578–589 (2018)..

Takeda, M., Ina, H.&Kobayashi, S. Fourier-transform method of fringe-pattern analysis for computer-based topography and interferometry.J. Opt. Soc. Am.72, 156–160 (1982)..

Wolf, E. Three-dimensional structure determination of semi-transparent objects from holographic data.Opt. Commun.1, 153–156 (1969)..

Devaney, A. J. Inverse-scattering theory within the Rytov approximation.Opt. Lett.6, 374–376 (1981)..

Yu, H. et al. Fourier-transform light scattering of individual colloidal clusters.Opt. Lett.37, 2577–2579 (2012)..

Lim, J., Ayoub, A. B.&Psaltis, D. Three-dimensional tomography of red blood cells using deep learning.Adv. Photonics2, 026001 (2020)..

Senyuk, B. et al. Topological colloids.Nature493, 200–205 (2013)..

Jung, J. et al. Measurements of polarization-dependent angle-resolved light scattering from individual microscopic samples using Fourier transform light scattering.Opt. Express26, 7701–7711 (2018)..

Sauter, A. et al. Real-time observation of nonclassical protein crystallization kinetics.J. Am. Chem. Soc.137, 1485–1491 (2015)..

Yoon, J. et al. Identification of non-activated lymphocytes using three-dimensional refractive index tomography and machine learning.Sci. Rep.7, 6654 (2017)..

Bennet, M. et al. A bacteria-based remotely tunable photonic device.Adv. Opt. Mater.5, 1600617 (2017)..

Kim, K. et al. Real-time visualization of 3-D dynamic microscopic objects using optical diffraction tomography.Opt. Express21, 32269–32278 (2013)..

Kim, D. et al. Large-scale uniform optical focus array generation with a phase spatial light modulator.Opt. Lett.44, 3178–3181 (2019)..

Lee, M., Shin, S.&Park, Y. Reconstructions of refractive index tomograms via a discrete algebraic reconstruction technique.Opt. Express25, 27415–27430 (2017)..

Kamilov, U. S. et al. Learning approach to optical tomography.Optica2, 517–522 (2015)..

Soto, J. M., Rodrigo, J. A.&Alieva, T. Partially coherent illumination engineering for enhanced refractive index tomography.Opt. Lett.43, 4699–4702 (2018)..

Chen, X. et al. Wolf phase tomography (WPT) of transparent structures using partially coherent illumination.Light: Sci. Appl.9, 142 (2020)..

Baek, Y.&Park, Y. Intensity-based holographic imaging via space-domain Kramers–Kronig relations.Nat. Photonics1–7, (2021)https://doi.org/10.1038/s41566-021-00760-8https://doi.org/10.1038/s41566-021-00760-8..

Fan, S. L. et al. Reconstructing complex refractive-index of multiply-scattering media by use of iterative optical diffraction tomography.Opt. Express28, 6846–6858 (2020)..

Freudiger, C. W. et al. Label-free biomedical imaging with high sensitivity by stimulated raman scattering microscopy.Science322, 1857–1861 (2008)..

Lee, M. et al. Deep-learning based three-dimensional label-free tracking and analysis of immunological synapses of CAR-T cells.eLife9, e49023 (2020)..

Zhang, Z. et al. Far-field diffraction microscopy at λ/10 resolution.Optica3, 609–612 (2016)..

Gerchberg, R. W. Super-resolution through error energy reduction.Opt. Acta21, 709–720 (1974)..

Shin, S. et al. Active illumination using a digital micromirror device for quantitative phase imaging.Opt. Lett.40, 5407–5410 (2015)..

Lee, K. et al. Time-multiplexed structured illumination using a DMD for optical diffraction tomography.Opt. Lett.42, 999–1002 (2017)..

Hale, G. M.&Querry, M. R. Optical constants of water in the 200-nm to 200-μm wavelength region.Appl. Opt.12, 555–563 (1973)..

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