In vivo flow cytometry reveals a circadian rhythm of circulating tumor cells

Xi Zhu; Yuanzhen Suo; Yuting Fu; Fuli Zhang; Nan Ding; Kai Pang; Chengying Xie; Xiaofu Weng; Meilu Tian; Hao He; Xunbin Wei

doi:10.1038/s41377-021-00542-5

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In vivo flow cytometry reveals a circadian rhythm of circulating tumor cells

Articles

- In vivo flow cytometry reveals a circadian rhythm of circulating tumor cells
- Light: Science & Applications Vol. 10, Issue 6, Pages: 1112-1121(2021)
- Affiliations：
  
  1.State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, 200030 Shanghai, China
  2.Biomedical Pioneering Innovation Center, Peking University, 100871 Beijing, China
  3.School of Life Sciences, Peking University, 100871 Beijing, China
  4.School of Instrument Science and Optoelectronics Engineering, Beijing Information Science and Technology University, 100192 Beijing, China
  5.Biomedical Engineering Department, Peking University, 100081 Beijing, China
  6.Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital and Institute, 100142 Beijing, China
- Author bio：
  
  Yuanzhen Suo (suoyuanzhen@pku.edu.cn)
  Hao He (haohe@sjtu.edu.cn)
  Xunbin Wei (xwei01@sjtu.edu.cn)
- Funds：
- DOI：10.1038/s41377-021-00542-5
  CLC：
- Published：30 June 2021，
  
  Published Online：28 May 2021，
  
  Received：18 November 2020，
  
  Revised：21 April 2021，
  
  Accepted：21 April 2021
- Accepted：
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Zhu, X. et al. In vivo flow cytometry reveals a circadian rhythm of circulating tumor cells. Light: Science & Applications, 10, 1112-1121 (2021).
DOI：

Zhu, X. et al. In vivo flow cytometry reveals a circadian rhythm of circulating tumor cells. Light: Science & Applications, 10, 1112-1121 (2021). DOI： 10.1038/s41377-021-00542-5.

摘要

Abstract

Circulating tumor cells (CTCs) is an established biomarker of cancer metastasis. The circulation dynamics of CTCs are important for understanding the mechanisms underlying tumor cell dissemination. Although studies have revealed that the circadian rhythm may disrupt the growth of tumors

it is generally unclear whether the circadian rhythm controls the release of CTCs. In clinical examinations

the current in vitro methods for detecting CTCs in blood samples are based on a fundamental assumption that CTC counts in the peripheral blood do not change significantly over time

which is being challenged by recent studies. Since it is not practical to draw blood from patients repeatedly

a feasible strategy to investigate the circadian rhythm of CTCs is to monitor them by in vivo detection methods. Fluorescence in vivo flow cytometry (IVFC) is a powerful optical technique that is able to detect fluorescent circulating cells directly in living animals in a noninvasive manner over a long period of time. In this study

we applied fluorescence IVFC to monitor CTCs noninvasively in an orthotopic mouse model of human prostate cancer. We observed that CTCs exhibited stochastic bursts over cancer progression. The probability of the bursting activity was higher at early stages than at late stages. We longitudinally monitored CTCs over a 24-h period

and our results revealed striking daily oscillations in CTC counts that peaked at the onset of the night (active phase for rodents)

suggesting that the release of CTCs might be regulated by the circadian rhythm.

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references

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