Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography

Zhe Wang; Vittorio Bianco; Daniele Pirone; Pasquale Memmolo; Massimiliano Maria Villone; Pier Luca Maffettone; Pietro Ferraro

doi:10.1038/s41377-021-00626-2

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Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography

Articles

- Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography
- Light: Science & Applications Vol. 10, Issue 10, Pages: 1889-1904(2021)
- Affiliations：
  
  1.Dipartimento di Ingegneria Chimica dei Materiali e della Produzione Industriale, Università degli Studi di Napoli "Federico II", Piazzale Tecchio 80, 80125, Napoli, Italy
  2.NEAPoLIS, Numerical and Experimental Advanced Program on Liquids and Interface Systems, Joint Research Center CNR - Università degli Studi di Napoli "Federico II", Napoli, Italy
  3.CNR-ISASI, Institute of Applied Sciences and Intelligent Systems "E. Caianiello", Via Campi Flegrei 34, 80078 Pozzuoli, Napoli, Italy
  4.Dipartimento di Ingegneria Elettrica e delle Tecnologie dell'Informazione, Università degli Studi di Napoli "Federico II", via Claudio 21, 80125, Napoli, Italy
- Author bio：
  
  Pasquale Memmolo (pasquale.memmolo@isasi.cnr.it)
  Pietro Ferraro (pietro.ferraro@cnr.it)
- Funds：
- DOI：10.1038/s41377-021-00626-2
  CLC：
- Published：31 October 2021，
  
  Published Online：15 September 2021，
  
  Received：26 January 2021，
  
  Revised：15 July 2021，
  
  Accepted：27 August 2021
- Accepted：
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Wang, Z. et al. Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography. Light: Science & Applications, 10, 1889-1904 (2021).
DOI：

Wang, Z. et al. Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography. Light: Science & Applications, 10, 1889-1904 (2021). DOI： 10.1038/s41377-021-00626-2.

摘要

Abstract

Single-cell phase-contrast tomography promises to become decisive for studying 3D intracellular structures in biology. It involves probing cells with light at wide angles

which unfortunately requires complex systems. Here we show an intriguing concept based on an inherent natural process for plants biology

i.e.

dehydration

allowing us to easily obtain 3D-tomography of onion-epidermal cells' nuclei. In fact

the loss of water reduces the turgor pressure and we recognize it induces significant rotation of cells' nuclei. Thanks to the holographic focusing flexibility and an ad-hoc angles' tracking algorithm

we combine different phase-contrast views of the nuclei to retrieve their 3D refractive index distribution. Nucleolus identification capability and a strategy for measuring morphology

dry mass

biovolume

and refractive index statistics are reported and discussed. This new concept could revolutionize the investigation in plant biology by enabling dynamic 3D quantitative and label-free analysis at sub-nuclear level using a conventional holographic setup.

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