Mirror-enhanced scanning light-field microscopy for long-term high-speed 3D imaging with isotropic resolution

Bo Xiong; Tianyi Zhu; Yuhan Xiang; Xiaopeng Li; Jinqiang Yu; Zheng Jiang; Yihan Niu; Dong Jiang; Xu Zhang; Lu Fang; Jiamin Wu; Qionghai Dai

doi:10.1038/s41377-021-00665-9

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Mirror-enhanced scanning light-field microscopy for long-term high-speed 3D imaging with isotropic resolution

Articles

- Mirror-enhanced scanning light-field microscopy for long-term high-speed 3D imaging with isotropic resolution
- Light: Science & Applications Vol. 10, Issue 12, Pages: 2369-2379(2021)
- Affiliations：
  
  1.Department of Automation, Tsinghua University, Beijing 100084, China
  2.Institute for Brain and Cognitive Sciences, Tsinghua University, Beijing, 100084 China
  3.Beijing Laboratory of Brain and Cognitive Intelligence, Beijing Municipal Education Commission, Beijing 100084, China
  4.State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Centre for Life Sciences, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing 100084, China
  5.Beijing Institute of Collaborative Innovation, Beijing 100094, China
  6.Department of Electronic Engineering, Tsinghua University, Beijing 100084, China
- Author bio：
  
  Lu Fang (fanglu@tsinghua.edu.cn)
  Jiamin Wu (wujiamin@tsinghua.edu.cn)
  Qionghai Dai (daiqh@tsinghua.edu.cn)
- Funds：
- DOI：10.1038/s41377-021-00665-9
  CLC：
- Published：31 December 2021，
  
  Published Online：04 November 2021，
  
  Received：30 April 2021，
  
  Revised：05 October 2021，
  
  Accepted：18 October 2021
- Accepted：
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Xiong, B. et al. Mirror-enhanced scanning light-field microscopy for long-term high-speed 3D imaging with isotropic resolution. Light: Science & Applications, 10, 2369-2379 (2021).
DOI：

Xiong, B. et al. Mirror-enhanced scanning light-field microscopy for long-term high-speed 3D imaging with isotropic resolution. Light: Science & Applications, 10, 2369-2379 (2021). DOI： 10.1038/s41377-021-00665-9.

摘要

Abstract

Various biological behaviors can only be observed in 3D at high speed over the long term with low phototoxicity. Light-field microscopy (LFM) provides an elegant compact solution to record 3D information in a tomographic manner simultaneously

which can facilitate high photon efficiency. However

LFM still suffers from the missing-cone problem

leading to degraded axial resolution and ringing effects after deconvolution. Here

we propose a mirror-enhanced scanning LFM (MiSLFM) to achieve long-term high-speed 3D imaging at super-resolved axial resolution with a single objective

by fully exploiting the extended depth of field of LFM with a tilted mirror placed below samples. To establish the unique capabilities of MiSLFM

we performed extensive experiments

we observed various organelle interactions and intercellular interactions in different types of photosensitive cells under extremely low light conditions. Moreover

we demonstrated that superior axial resolution facilitates more robust blood cell tracking in zebrafish larvae at high speed.

关键词

Keywords

references

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