Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration

Dong-Yu Li; Qing Xia; Ting-Ting Yu; Jing-Tan Zhu; Dan Zhu

doi:10.1038/s41377-021-00682-8

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Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration

Articles

- Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration
- Light: Science & Applications Vol. 10, Issue 12, Pages: 2492-2506(2021)
- Affiliations：
  
  1.Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 430074 Wuhan, Hubei, China
  2.MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, 430074 Wuhan, Hubei, China
- Author bio：
  
  Dan Zhu (dawnzh@mail.hust.edu.cn)
- Funds：
- DOI：10.1038/s41377-021-00682-8
  CLC：
- Published：31 December 2021，
  
  Published Online：03 December 2021，
  
  Received：25 June 2021，
  
  Revised：20 October 2021，
  
  Accepted：23 November 2021
- Accepted：
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Li, D. Y. et al. Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration. Light: Science & Applications, 10, 2492-2506 (2021).
DOI：

Li, D. Y. et al. Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration. Light: Science & Applications, 10, 2492-2506 (2021). DOI： 10.1038/s41377-021-00682-8.

摘要

Abstract

Laser speckle contrast imaging (LSCI) is a powerful tool to monitor blood flow distribution and has been widely used in studies of microcirculation

both for animal and clinical applications. Conventionally

LSCI usually works on reflective-detected mode. However

it could provide promising temporal and spatial resolution for in vivo applications only with the assistance of various tissue windows

otherwise

the overlarge superficial static speckle would extremely limit its contrast and resolution. Here

we systematically investigated the capability of transmissive-detected LSCI (TR-LSCI) for blood flow monitoring in thick tissue. Using Monte Carlo simulation

we theoretically compared the performance of transmissive and reflective detection. It was found that the reflective-detected mode was better when the target layer was at the very surface

but the imaging quality would rapidly decrease with imaging depth

while the transmissive-detected mode could obtain a much stronger signal-to-background ratio (SBR) for thick tissue. We further proved by tissue phantom

animal

and human experiments that in a certain thickness of tissue

TR-LSCI showed remarkably better performance for thick-tissue imaging

and the imaging quality would be further improved if the use of longer wavelengths of near-infrared light. Therefore

both theoretical and experimental results demonstrate that TR-LSCI is capable of obtaining thick-tissue blood flow information and holds great potential in the field of microcirculation research.

关键词

Keywords

references

Stern, M. D. In vivo evaluation of microcirculation by coherent light scattering.Nature254, 56–58 (1975)..

Mennes, O. A. et al. Assessment of microcirculation in the diabetic foot with laser speckle contrast imaging.Physiological Meas.40, 065002 (2019)..

Gnyawali, S. C. et al. Quantification of cerebral perfusion using laser speckle imaging and infarct volume using MRI in a pre-clinical model of posterior circulation stroke.J. Vis. Exp.https://doi.org/10.3791/61673https://doi.org/10.3791/61673(2021)..

Ma, J. Q. et al. Impaired collateral flow in pial arterioles of aged rats during ischemic stroke.Transl. Stroke Res.11, 243–253 (2021)..

Shemesh, D. et al. Decreased cerebral blood flow and hemodynamic parameters during acute hyperglycemia in mice model observed by dual-wavelength speckle imaging.J. Biophotonics12, e201900002 (2019)..

Roustit, M.&Cracowski, J. L. Assessment of endothelial and neurovascular function in human skin microcirculation.Trends Pharmacol. Sci.34, 373–384 (2013)..

Hedhli, J. et al. Imaging the landmarks of vascular recovery.Theranostics10, 1733–1745 (2021)..

Feng, W. et al. Comparison of cerebral and cutaneous microvascular dysfunction with the development of type 1 diabetes.Theranostics9, 5854–5868 (2019)..

Schrandt, C. J. et al. Chronic monitoring of vascular progression after ischemic stroke using multiexposure speckle imaging and two-photon fluorescence microscopy.J. Cereb. Blood Flow. Metab.35, 933–942 (2015)..

Tennant, K. A.&Brown, C. E. Diabetes augments in vivo microvascular blood flow dynamics after stroke.J. Neurosci.33, 19194–19204 (2013)..

Sorop, O. et al. Coronary microvascular dysfunction results in impaired coronary flow reserve and altered oxygen balance in a swine model of INOCA with multiple risk factors.Eur. Heart J.41, 3781–3781 (2021)..

Anderson, C. P., Pekas, E. J.&Park, S. Y. Microvascular dysfunction in peripheral artery disease: is heat therapy a viable treatment?Int. J. Environ. Res. Public Health18, 2384 (2021)..

Dunn, A. K. et al. Dynamic imaging of cerebral blood flow using laser speckle.J. Cereb. Blood Flow. Metab.21, 195–201 (2001)..

Kelly, A. et al. Microvascular effects of pulsed dye laser in combination with oxymetazoline.Lasers Surg. Med.52, 17–22 (2021)..

Wang, J. et al. Assessment of optical clearing induced improvement of laser speckle contrast imaging.J. Innovative Optical Health Sci.3, 159–167 (2010)..

Alexandrou, M. E. et al. Haemodialysis and peritoneal dialysis patients have severely impaired post-occlusive skin forearm vasodilatory response assessed with laser speckle contrast imaging.Clin. Kidney J.14, 1419–1427 (2021)..

Brinca, A., Pinho, A.&Vieira, R. Laser speckle contrast imaging for assessment of human skin graft microcirculation.J. Eur. Acad. Dermatol. Venereol.34, e491–e493 (2021)..

Tenland, K. et al. Successful free bilamellar eyelid grafts for the repair of upper and lower eyelid defects in patients and laser speckle contrast imaging of revascularization.Ophthalmic Plast. Reconstructive Surg.37, 168–172 (2021)..

Cheng, H. Y. et al. Modified laser speckle imaging method with improved spatial resolution.J. Biomed. Opt.8, 559–564 (2003)..

Dunn, J. F. et al. A transmissive laser speckle imaging technique for measuring deep tissue blood flow: an example application in finger joints.Lasers Surg. Med.43, 21–28 (2011)..

Meisner, J. K. et al. Trans-illuminated laser speckle imaging of collateral artery blood flow in ischemic mouse hindlimb.J. Biomed. Opt.18, 096011 (2013)..

Wang, L. H., Jacques, S. L.&Zheng, L. Q. MCML—Monte Carlo modeling of light transport in multi-layered tissues.Computer Methods Prog. Biomedicine47, 131–146 (1995)..

Kirkpatrick, S. J., Duncan, D. D.&Wells-Gray, E. M. Detrimental effects of speckle-pixel size matching in laser speckle contrast imaging.Opt. Lett.33, 2886–2888 (2008)..

Postnov, D. D. et al. Dynamic light scattering imaging.Sci. Adv.6, eabc4628 (2021)..

Yousefi, S. et al. Assessment of microcirculation dynamics during cutaneous wound healing phases in vivo using optical microangiography.J. Biomed. Opt.19, 076015 (2014)..

Arpino, J. M. et al. Four-dimensional microvascular analysis reveals that regenerative angiogenesis in ischemic muscle produces a flawed microcirculation.Circulation Res.120, 1453–1465 (2017)..

Feng, W. et al. Visualization of skin microvascular dysfunction of type 1 diabetic mice using in vivo skin optical clearing method.J. Biomed. Opt.24, 031003 (2018)..

Ahrari, F. et al. The efficacy of low-level laser therapy for the treatment of myogenous temporomandibular joint disorder.Lasers Med. Sci.29, 551–557 (2014)..

Dphil, B. M. et al. Double-blind, placebo-controlled investigation of the effect of combined phototherapy/low intensity laser therapy upon experimental ischaemic pain in humans.Lasers Surg. Med.17, 74–81 (1995)..

Zhang, B. M. The Transmission Characteristics Analysis of Polarization Light In Scattering Medium. MSc thesis, Hefei University of Technology, Hefei (2018).

Shen, F. et al. The depolarization performances of the polarized light in different scattering media systems.IEEE Photonics J.10, 3900212 (2018)..

Davis, M. A. et al. Sensitivity of laser speckle contrast imaging to flow perturbations in the cortex.Biomed. Opt. Express7, 759–775 (2016)..

Maeda, A. et al. In vivo imaging reveals significant tumor vascular dysfunction and increased tumor hypoxia-inducible factor-1α expression induced by high single-dose irradiation in a pancreatic tumor model.Int. J. Radiat. Oncol. ·Biol. Phys.97, 184–194 (2017)..

Tong, F. et al. Hypo-fractionation radiotherapy normalizes tumor vasculature in non-small cell lung cancer xenografts through the p-STAT3/HIF-1 alpha signaling pathway.Therapeutic Adv. Med. Oncol.12, 1–12 (2021)..

Machado, M. J. C.&Mitchell, C. A. Temporal changes in microvessel leakiness during wound healing discriminated by in vivo fluorescence recovery after photobleaching.J. Physiol.589, 4681–4696 (2011)..

Boulaftali, Y.et al. The mouse dorsal skinfold chamber as a model for the study of thrombolysis by intravital microscopy.Thrombosis Haemost.107, 962–971 (2012)..

Crescente, M. et al. ADAMTS13 exerts a thrombolytic effect in microcirculation.Thrombosis Haemost.108, 527–532 (2012)..

Xing, L. Z. et al. Nd: YAG laser combined with gold nanorods for potential application in port-wine stains: an in vivo study.J. Biomed. Opt.22, 115005 (2017)..

Li, D. et al. Experimental study on the vascular thermal response to visible laser pulses.Lasers Med. Sci.30, 135–145 (2015)..

Moy, W. J. et al. Hemoporfin-mediated photodynamic therapy on normal vasculature: implications for phototherapy of port-wine stain birthmarks.J. Clin. Transl. Res.2, 107–111 (2016)..

Finžgar, M. et al. Transcutaneous application of the gaseous CO2for improvement of the microvascular function in patients with diabetic foot ulcers.Microvascular Res.133, 104100 (2021)..

Dávida, L. et al. A prospective, longitudinal monocentric study on laser Doppler imaging of microcirculation: comparison with macrovascular pathophysiology and effect of adalimumab treatment in early rheumatoid arthritis.Rheumatol. Int.40, 415–424 (2021)..

Blatter, C. et al. In situ structural and microangiographic assessment of human skin lesions with high-speed OCT.Biomed. Opt. Express3, 2636–2646 (2012)..

Li, D. Y. et al. Visible-near infrared-Ⅱ skull optical clearing window for in vivo cortical vasculature imaging and targeted manipulation.J. Biophotonics13, e202100142 (2021)..

Vo-Dinh, T.&Cullum, B. M.Biomedical Photonics Handbook(CRC Press, 2003).

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