Lipid droplets as endogenous intracellular microlenses

Xixi Chen; Tianli Wu; Zhiyong Gong; Jinghui Guo; Xiaoshuai Liu; Yao Zhang; Yuchao Li; Pietro Ferraro; Baojun Li

doi:10.1038/s41377-021-00687-3

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Lipid droplets as endogenous intracellular microlenses

Articles

- Lipid droplets as endogenous intracellular microlenses
- Light: Science & Applications Vol. 10, Issue 12, Pages: 2507-2517(2021)
- Affiliations：
  
  1.Institute of Nanophotonics, Jinan University, 511443 Guangzhou, China
  2.Department of Physiology, School of Medicine, Jinan University, 510632 Guangzhou, China
  3.CNR-ISASI, Institute of Applied Sciences and Intelligent Systems «E. Caianiello», Via Campi Flegrei 34, 80078 Pozzuoli, Naples, Italy
- Author bio：
  
  Yao Zhang (zhyao5@jnu.edu.cn)
  Yuchao Li (liyuchao@jnu.edu.cn)
  Pietro Ferraro (pietro.ferraro@cnr.it)
  Baojun Li (baojunli@jnu.edu.cn)
- Funds：
- DOI：10.1038/s41377-021-00687-3
  CLC：
- Published：31 December 2021，
  
  Published Online：06 December 2021，
  
  Received：27 June 2021，
  
  Revised：31 October 2021，
  
  Accepted：23 November 2021
- Accepted：
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Chen, X. X. et al. Lipid droplets as endogenous intracellular microlenses. Light: Science & Applications, 10, 2507-2517 (2021).
DOI：

Chen, X. X. et al. Lipid droplets as endogenous intracellular microlenses. Light: Science & Applications, 10, 2507-2517 (2021). DOI： 10.1038/s41377-021-00687-3.

摘要

Abstract

Using a single biological element as a photonic component with well-defined features has become a new intriguing paradigm in biophotonics. Here we show that endogenous lipid droplets in the mature adipose cells can behave as fully biocompatible microlenses to strengthen the ability of microscopic imaging as well as detecting intra- and extracellular signals. By the assistance of biolenses made of the lipid droplets

enhanced fluorescence imaging of cytoskeleton

lysosomes

and adenoviruses has been achieved. At the same time

we demonstrated that the required excitation power can be reduced by up to 73%. The lipidic microlenses are finely manipulated by optical tweezers in order to address targets and perform their real-time imaging inside the cells. An efficient detecting of fluorescence signal of cancer cells in extracellular fluid was accomplished due to the focusing effect of incident light by the lipid droplets. The lipid droplets acting as endogenous intracellular microlenses open the intriguing route for a multifunctional biocompatible optics tool for biosensing

endoscopic imaging

and single-cell diagnosis.

关键词

Keywords

references

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