One step synthesis of efficient red emissive carbon dots and their bovine serum albumin composites with enhanced multi-photon fluorescence for in vivo bioimaging

Huiqi Zhang; Gang Wang; Zhiming Zhang; Josh Haipeng Lei; Tzu-Ming Liu; Guichuan Xing; Chu-Xia Deng; Zikang Tang; Songnan Qu

doi:10.1038/s41377-022-00798-5

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One step synthesis of efficient red emissive carbon dots and their bovine serum albumin composites with enhanced multi-photon fluorescence for in vivo bioimaging

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- One step synthesis of efficient red emissive carbon dots and their bovine serum albumin composites with enhanced multi-photon fluorescence for in vivo bioimaging
- Light: Science & Applications Vol. 11, Issue 5, Pages: 955-968(2022)
- Affiliations：
  
  1.Joint Key Laboratory of the Ministry of Education, Institute of Applied Physics and Materials Engineering, University of Macau, Macau, 999078, China
  2.Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau, 999078, China
  3.MoE Frontiers Science Center for Precision Oncology, University of Macau, Macau, 999078, China
  4.Cancer Center, Faculty of Health Sciences, University of Macau, Macau, 999078, China
- Author bio：
  
  Songnan Qu (songnanqu@um.edu.mo)
- Funds：
- DOI：10.1038/s41377-022-00798-5
  CLC：
- Published：31 May 2022，
  
  Published Online：27 April 2022，
  
  Received：25 January 2022，
  
  Revised：05 April 2022，
  
  Accepted：11 April 2022
- Accepted：
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Zhang, H. Q. et al. One step synthesis of efficient red emissive carbon dots and their bovine serum albumin composites with enhanced multi-photon fluorescence for in vivo bioimaging. Light: Science & Applications, 11, 955-968 (2022).
DOI：

Zhang, H. Q. et al. One step synthesis of efficient red emissive carbon dots and their bovine serum albumin composites with enhanced multi-photon fluorescence for in vivo bioimaging. Light: Science & Applications, 11, 955-968 (2022). DOI： 10.1038/s41377-022-00798-5.

摘要

Abstract

Efficient red emissive carbon dots (CDs) in aqueous solutions are very scarce for high performance bioimaging applications. In this work

we report a one-step solvothermal treatment to synthesize pure red emissive CDs (FA-CDs) from citric acid and urea in formic acid without complicated purification procedures. Photoluminescence quantum yield (PLQY) of 43.4% was observed in their dimethyl sulfoxide solutions. High PLQY up to 21.9% in aqueous solutions was achieved in their bovine serum albumin (BSA) composites (FA-CDs@BSA) with significantly enhanced multi-photon fluorescence. The strong surface electron-withdrawing structure of FA-CDs caused by the high content of C = O groups contributes for their pure red emission. Owing to the significantly enhanced single and multi-photon red fluorescence and enlarged particle sizes after composing with BSA

in vivo tumor imaging and two-photon fluorescence imaging of blood vessels in mouse ear have been realized via intravenous injection of FA-CDs@BSA aqueous solutions.

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references

Ye, R. Q. et al. Coal as an abundant source of graphene quantum dots.Nat. Commun.4, 2943 (2013)..

Lim, S. Y., Shen, W.&Gao, Z. Q. Carbon quantum dots and their applications.Chem. Soc. Rev.44, 362–381 (2015)..

Lesani, P. et al. Design principles and biological applications of red-emissive two-photon carbon dots.Commun. Mater.2, 108 (2021)..

Zhu, S. J. et al. Surface chemistry routes to modulate the photoluminescence of graphene quantum dots: from fluorescence mechanism to up-conversion bioimaging applications.Adv. Funct. Mater.22, 4732–4740 (2012)..

Liu, Y. et al. One-step synthesis of robust nitrogen-doped carbon dots: acid-evoked fluorescence enhancement and their application in Fe3+detection.J. Mater. Chem. A3, 17747–17754 (2015)..

Jiang, L. et al. UV–Vis–NIR full-range responsive carbon dots with large multiphoton absorption cross sections and deep-red fluorescence at nucleoli and in vivo.Small16, 2000680 (2020)..

Zhang, T. X. et al. A novel mechanism for red emission carbon dots: hydrogen bond dominated molecular states emission.Nanoscale9, 13042–13051 (2017)..

Li, S. H. et al. Targeted tumour theranostics in mice via carbon quantum dots structurally mimicking large amino acids.Nat. Biomed. Eng.4, 704–716 (2020)..

Lu, S. Y. et al. Near-infrared photoluminescent polymer–carbon nanodots with two-photon fluorescence.Adv. Mater.29, 1603443 (2017)..

Liu, K. K. et al. Efficient red/near-infrared-emissive carbon nanodots with multiphoton excited upconversion fluorescence.Adv. Sci.6, 1900766 (2019)..

Karakoçak, B. B. et al. Optimizing the synthesis of red-emissive nitrogen-doped carbon dots for use in bioimaging.ACS Appl. Nano Mater.1, 3682–3692 (2018)..

Holá, K. et al. Graphitic nitrogen triggers red fluorescence in carbon dots.ACS Nano11, 12402–12410 (2017)..

Lan, M. H. et al. Two-photon-excited near-infrared emissive carbon dots as multifunctional agents for fluorescence imaging and photothermal therapy.Nano Res.10, 3113–3123 (2017)..

Jiang, K. et al. Preparation of multicolor photoluminescent carbon dots by tuning surface states.Nanomaterials9, 529 (2019)..

Pan, L. L. et al. Near-infrared emissive carbon dots for two-photon fluorescence bioimaging.Nanoscale8, 17350–17356 (2016)..

Qu, S. N. et al. Toward efficient orange emissive carbon nanodots through conjugated sp2-domain controlling and surface charges engineering.Adv. Mater.28, 3516–3521 (2016)..

Li, D. et al. Near-infrared excitation/emission and multiphoton-induced fluorescence of carbon dots.Adv. Mater.30, 1705913 (2018)..

Liu, Y. F. et al. Rational synthesis of highly efficient ultra-narrow red-emitting carbon quantum dots forNIR-Ⅱ two-photon bioimaging.Nanoscale12, 1589–1601 (2020)..

Cao, L. et al. Carbon dots for multiphoton bioimaging.J. Am. Chem. Soc.129, 11318–11319 (2007)..

Ye, X. X. et al. A red emissive two-photon fluorescence probe based on carbon dots for intracellular pH detection.Small15, 1901673 (2019)..

Bai, Y. L. et al. Carbon dots with absorption red-shifting for two-photon fluorescence imaging of tumor tissue pH and synergistic phototherapy.ACS Appl. Mater. Interfaces13, 35365–35375 (2021)..

Li, L. et al. Kilogram-scale synthesis and functionalization of carbon dots for superior electrochemical potassium storage.ACS Nano15, 6872–6885 (2021)..

Tang, L. B. et al. Deep ultraviolet to near-infrared emission and photoresponse in layered N-doped graphene quantum dots.ACS Nano8, 6312–6320 (2014)..

Bhattacharya, S. et al. Fluorescent self-healing carbon dot/polymer gels.ACS Nano13, 1433–1442 (2019)..

Yu, H. W. et al. Preparation of carbon dots by non-focusing pulsed laser irradiation in toluene.Chem. Commun.52, 819–822 (2016)..

Xu, X. K. et al. Construction of carbon dots with color-tunable aggregation-induced emission by nitrogen-induced intramolecular charge transfer.Adv. Mater.33, 2104872 (2021)..

Jia, H. R. et al. Electroluminescent warm white light-emitting diodes based on passivation enabled bright red bandgap emission carbon quantum dots.Adv. Sci.6, 1900397 (2019)..

Liu, E. S. et al. Enhanced near-infrared emission from carbon dots by surface deprotonation.J. Phys. Chem. Lett.12, 604–611 (2021)..

Zhang, J. X., Zheng, M.&Xie, Z. G. Co-assembled hybrids of proteins and carbon dots for intracellular protein delivery.J. Mater. Chem. B4, 5659–5663 (2016)..

Gospodarczyk, W., Szutkowski, K.&Kozak, M. Interaction of bovine serum albumin (BSA) with novel Gemini surfactants studied by synchrotron radiation scattering (SR-SAXS), circular dichroism (CD), and nuclear magnetic resonance (NMR).J. Phys. Chem. B118, 8652–8661 (2014)..

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