Transport of intensity diffraction tomography with non-interferometric synthetic aperture for three-dimensional label-free microscopy

Jiaji Li; Ning Zhou; Jiasong Sun; Shun Zhou; Zhidong Bai; Linpeng Lu; Qian Chen; Chao Zuo

doi:10.1038/s41377-022-00815-7

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Transport of intensity diffraction tomography with non-interferometric synthetic aperture for three-dimensional label-free microscopy

Original Articles

- Transport of intensity diffraction tomography with non-interferometric synthetic aperture for three-dimensional label-free microscopy
- Light: Science & Applications Vol. 11, Issue 10, Pages: 2294-2307(2022)
- Affiliations：
  
  1.School of Electronic and Optical Engineering, Nanjing University of Science and Technology, No. 200 Xiaolingwei Street, Nanjing, Jiangsu Province, 210094, China
  2.Jiangsu Key Laboratory of Spectral Imaging & Intelligent Sense, Nanjing University of Science and Technology, Nanjing, Jiangsu Province, 210094, China
  3.Smart Computational Imaging Laboratory (SCILab), Nanjing University of Science and Technology, Nanjing, Jiangsu Province, 210094, China
- Author bio：
  
  Qian Chen (chenqian@njust.edu.cn)
  Chao Zuo (zuochao@njust.edu.cn)
- Funds：
- DOI：10.1038/s41377-022-00815-7
  CLC：
- Published：31 October 2022，
  
  Published Online：02 June 2022，
  
  Received：25 November 2021，
  
  Revised：12 April 2022，
  
  Accepted：24 April 2022
- Accepted：
Scan QR Code
Li, J. J. et al. Transport of intensity diffraction tomography with non-interferometric synthetic aperture for three-dimensional label-free microscopy. Light: Science & Applications, 11, 2294-2307 (2022).
DOI：

Li, J. J. et al. Transport of intensity diffraction tomography with non-interferometric synthetic aperture for three-dimensional label-free microscopy. Light: Science & Applications, 11, 2294-2307 (2022). DOI： 10.1038/s41377-022-00815-7.

摘要

Abstract

We present a new label-free three-dimensional (3D) microscopy technique

termed transport of intensity diffraction tomography with non-interferometric synthetic aperture (TIDT-NSA). Without resorting to interferometric detection

TIDT-NSA retrieves the 3D refractive index (RI) distribution of biological specimens from 3D intensity-only measurements at various illumination angles

allowing incoherent-diffraction-limited quantitative 3D phase-contrast imaging. The unique combination of z-scanning the sample with illumination angle diversity in TIDT-NSA provides strong defocus phase contrast and better optical sectioning capabilities suitable for high-resolution tomography of thick biological samples. Based on an off-the-shelf bright-field microscope with a programmable light-emitting-diode (LED) illumination source

TIDT-NSA achieves an imaging resolution of 206 nm laterally and 520 nm axially with a high-NA oil immersion objective. We validate the 3D RI tomographic imaging performance on various unlabeled fixed and live samples

including human breast cancer cell lines MCF-7

human hepatocyte carcinoma cell lines HepG2

mouse macrophage cell lines RAW 264.7

Caenorhabditis elegans

(

C. elegans

)

and live Henrietta Lacks (HeLa) cells. These results establish TIDT-NSA as a new non-interferometric approach to optical diffraction tomography and 3D label-free microscopy

permitting quantitative characterization of cell morphology and time-dependent subcellular changes for widespread biological and medical applications.

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Keywords

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