Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses

Agata M. Kotulska; Aleksandra Pilch-Wróbel; Satu Lahtinen; Tero Soukka; Artur Bednarkiewicz

doi:10.1038/s41377-022-00946-x

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Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses

Original Articles

- Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses
- Light: Science & Applications Vol. 11, Issue 10, Pages: 2321-2334(2022)
- Affiliations：
  
  1.Division of Biomedical Physicochemistry, Institute of Low Temperature and Structure Research, PAN, ul. Okolna 2, Wrocław 50-422, Poland
  2.Department of Life Technologies/Biotechnology, University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland
- Author bio：
  
  Tero Soukka (tejoso@utu.fi)
  Artur Bednarkiewicz (a.bednarkiewicz@intibs.pl)
- Funds：
- DOI：10.1038/s41377-022-00946-x
  CLC：
- Published：31 October 2022，
  
  Published Online：19 August 2022，
  
  Received：28 February 2022，
  
  Revised：03 July 2022，
  
  Accepted：25 July 2022
- Accepted：
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Kotulska, A. M. et al. Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses. Light: Science & Applications, 11, 2321-2334 (2022).
DOI：

Kotulska, A. M. et al. Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses. Light: Science & Applications, 11, 2321-2334 (2022). DOI： 10.1038/s41377-022-00946-x.

摘要

Abstract

Lanthanide-doped colloidal nanoparticles capable of photon upconversion (UC) offer long luminescence lifetimes

narrowband absorption and emission spectra

and efficient anti-Stokes emission. These features are highly advantageous for Förster Resonance Energy Transfer (FRET) based detection. Upconverting nanoparticles (UCNPs) as donors may solve the existing problems of molecular FRET systems

such as photobleaching and limitations in quantitative analysis

but these new labels also bring new challenges. Here we have studied the impact of the core-shell compositional architecture of upconverting nanoparticle donors and the mode of photoexcitation on the performance of UC-FRET from UCNPs to Rose Bengal (RB) molecular acceptor. We have quantitatively compared luminescence rise and decay kinetics of Er

emission using core-only NaYF

: 20% Yb

2% Er and core-shell NaYF

: 20% Yb @ NaYF

: 20% Yb

5% Er donor UCNPs under three photoexcitation schemes: (1) direct short-pulse photoexcitation of Er

at 520 nm; indirect photoexcitation of Er

through Yb

sensitizer with (2) 980 nm short (5–7 ns) or (3) 980 nm long (4 ms) laser pulses. The donor luminescence kinetics and steady-state emission spectra differed between the UCNP architectures and excitation schemes. Aiming for highly sensitive kinetic upconversion FRET-based biomolecular assays

the experimental results underline the complexity of the excitation and energy-migration mechanisms affecting the Er

donor responses and suggest ways to optimize the photoexcitation scheme and the architecture of the UCNPs used as luminescent donors.

关键词

Keywords

references

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