Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes

Xinzhu Xu; Shu Jia; Peng Xi

doi:10.1038/s41377-022-00983-6

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Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes

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- Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes
- Light: Science & Applications Vol. 11, Issue 10, Pages: 2271-2275(2022)
- Affiliations：
  
  1.Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing 100871, China
  2.Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA
  3.UTS-SUStech Joint Research Centre for Biomedical Materials & Devices, Department of Biomedical Engineering, College of Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China
  4.National Biomedical Imaging Center, Peking University, Beijing 100871, China
- Author bio：
  
  Peng Xi (xipeng@pku.edu.cn)
- Funds：
- DOI：10.1038/s41377-022-00983-6
  CLC：
- Published：31 October 2022，
  
  Published Online：10 October 2022，
- Accepted：
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Xu, X. Z., Jia, S. & Xi, P. Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes. Light: Science & Applications, 11, 2271-2275 (2022).
DOI：

Xu, X. Z., Jia, S. & Xi, P. Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes. Light: Science & Applications, 11, 2271-2275 (2022). DOI： 10.1038/s41377-022-00983-6.

摘要

Abstract

A donut excitation moves around a single molecule with a zigzag configuration lattice by lattice. Such a method implemented in scanning fluorescence microscopy simplifies the conventional MINFLUX process. Consisting of hollow zero-intensity excitation

single-pixel detection

time-correlated single photon counting

and drift stabilization

the system achieves localization precision and resolution very close to conventional MINFLUX theoretically and experimentally. An averaged high-SNR reference

and pixel-registered intensity from a single molecule is essential to reconstruct localization in maximum likelihood estimation. With performance reaching nearly conventional MINFLUX's

the proposed raster-scanning MINFLUX can inspire researchers expertized in STED or confocal setup to quickly transform to MINFLUX and develop for further exploring on bio-specimens or optical applications.

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Keywords

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