Non-invasive photoacoustic computed tomography of rat heart anatomy and function

Li Lin; Xin Tong; Susana Cavallero; Yide Zhang; Shuai Na; Rui Cao; Tzung K. Hsiai; Lihong V. Wang

doi:10.1038/s41377-022-01053-7

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Non-invasive photoacoustic computed tomography of rat heart anatomy and function

Original Articles

- Non-invasive photoacoustic computed tomography of rat heart anatomy and function
- Light: Science & Applications Vol. 12, Issue 1, Pages: 109-117(2023)
- Affiliations：
  
  1.Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology, Pasadena, CA, USA
  2.Department of Bioengineering, UCLA, Los Angeles, CA, USA
  3.Division of Cardiology, Department of Medicine, UCLA, Los Angeles, CA, USA
  4.Present address: College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, China
  5.Present address: The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
- Author bio：
  
  Tzung K. Hsiai (THsiai@mednet.ucla.edu)
  Lihong V. Wang (LVW@caltech.edu)
- Funds：
- DOI：10.1038/s41377-022-01053-7
  CLC：
- Published：31 January 2023，
  
  Published Online：3 January 2023，
  
  Received：11 July 2022，
  
  Revised：29 November 2022，
  
  Accepted：1 December 2022
Scan for full text
Lin, L. et al. Non-invasive photoacoustic computed tomography of rat heart anatomy and function. Light: Science & Applications, 12, 109-117 (2023).
DOI：

Lin, L. et al. Non-invasive photoacoustic computed tomography of rat heart anatomy and function. Light: Science & Applications, 12, 109-117 (2023). DOI： 10.1038/s41377-022-01053-7.

摘要

Abstract

Complementary to mainstream cardiac imaging modalities for preclinical research

photoacoustic computed tomography (PACT) can provide functional optical contrast with high imaging speed and resolution. However

PACT has not been demonstrated to reveal the dynamics of whole cardiac anatomy or vascular system without surgical procedure (thoracotomy) for tissue penetration. Here

we achieved non-invasive imaging of rat hearts using the recently developed three-dimensional PACT (3D-PACT) platform

demonstrating the regulated illumination and detection schemes to reduce the effects of optical attenuation and acoustic distortion through the chest wall; thereby

enabling unimpeded visualization of the cardiac anatomy and intracardiac hemodynamics following rapidly scanning the heart within 10 s. We further applied 3D-PACT to reveal distinct cardiac structural and functional changes among the healthy

hypertensive

and obese rats

with optical contrast to uncover differences in cardiac chamber size

wall thickness

and hemodynamics. Accordingly

3D-PACT provides high imaging speed and nonionizing penetration to capture the whole heart for diagnosing the animal models

holding promises for clinical translation to cardiac imaging of human neonates.

关键词

Keywords

references

Word Health Organization. The Top 10 Causes Of Death. https://www.who.int/news-room/fact-sheets/detail/the-top-10-causes-of-deathhttps://www.who.int/news-room/fact-sheets/detail/the-top-10-causes-of-death (2020).

I. J. Neeland , 等 . Effects of liraglutide on visceral and ectopic fat in adults with overweight and obesity at high cardiovascular risk: a randomised, double-blind, placebo-controlled, clinical trial . Lancet Diabetes Endocrinol. , 2021 . 9 595 -605 . DOI:10.1016/S2213-8587(21)00179-0http://doi.org/10.1016/S2213-8587(21)00179-0 .

F. Lobelo , 等 . Routine assessment and promotion of physical activity in healthcare settings: a scientific statement from the American Heart Association . Circulation , 2018 . 137 e495 -e522 . DOI:10.1161/CIR.0000000000000559http://doi.org/10.1161/CIR.0000000000000559 .

P. Fei , 等 . Cardiac light-sheet fluorescent microscopy for multi-scale and rapid imaging of architecture and function . Sci. Rep. , 2016 . 6 22489 DOI:10.1038/srep22489http://doi.org/10.1038/srep22489 .

V. Chetboul . Advanced techniques in echocardiography in small animals . Vet. Clin. North Am.: Small Anim. Pract. , 2010 . 40 529 -543 . DOI:10.1016/j.cvsm.2010.03.007http://doi.org/10.1016/j.cvsm.2010.03.007 .

M. Nahrendorf , 等 . Cardiac magnetic resonance imaging in small animal models of human heart failure . Med. Image Anal. , 2003 . 7 369 -375 . DOI:10.1016/S1361-8415(03)00011-2http://doi.org/10.1016/S1361-8415(03)00011-2 .

Bertolini, G. & Angeloni, L. Vascular and cardiac CT in small animals. in Computed Tomography-Advanced Applications (ed Halefoğlu, A. M. ) (Rijeka: IntechOpen, 2017).

Q. Wang , 等 . Application of animal and human PET in cardiac research . Am. J. Cardiovasc. Dis. , 2018 . 8 24 -30. .

R. Golestani , 等 . Small-animal SPECT and SPECT/CT: application in cardiovascular research . Eur. J. Nucl. Med. Mol. Imaging , 2010 . 37 1766 -1777 . DOI:10.1007/s00259-009-1321-8http://doi.org/10.1007/s00259-009-1321-8 .

W. Koba , L. A. Jelicks , E. J. Fine . MicroPET/SPECT/CT imaging of small animal models of disease . Am. J. Pathol. , 2013 . 182 319 -324 . DOI:10.1016/j.ajpath.2012.09.025http://doi.org/10.1016/j.ajpath.2012.09.025 .

C. Nanni , D. A. Torigian . Applications of small animal imaging with PET, PET/CT, and PET/MR imaging . PET Clin. , 2008 . 3 243 -250 . DOI:10.1016/j.cpet.2009.01.002http://doi.org/10.1016/j.cpet.2009.01.002 .

Izaguirre, E. W. et al. Dual modality micro-SPECT and micro-CT for small animal imaging: technical advances and challenges. Proceedings of the SPIE 5923, Penetrating Radiation Systems and Applications VII. (SPIE, 2005).

R. M. Botnar , M. R. Makowski . Cardiovascular magnetic resonance imaging in small animals . Prog. Mol. Biol. Transl. Sci. , 2012 . 105 227 -261 . DOI:10.1016/B978-0-12-394596-9.00008-1http://doi.org/10.1016/B978-0-12-394596-9.00008-1 .

J. J. Yao , J. Xia , L. V. Wang . Multiscale functional and molecular photoacoustic tomography . Ultrasonic Imaging , 2016 . 38 44 -62 . DOI:10.1177/0161734615584312http://doi.org/10.1177/0161734615584312 .

Z. J. Guo , L. Li , L. V. Wang . On the speckle-free nature of photoacoustic tomography . Med. Phys. , 2009 . 36 4084 -4088 . DOI:10.1118/1.3187231http://doi.org/10.1118/1.3187231 .

J. Weber , P. C. Beard , S. E. Bohndiek . Contrast agents for molecular photoacoustic imaging . Nat. Methods , 2016 . 13 639 -650 . DOI:10.1038/nmeth.3929http://doi.org/10.1038/nmeth.3929 .

X. L. Deán-Ben , 等 . Spiral volumetric optoacoustic tomography visualizes multi-scale dynamics in mice . Light Sci. Appl. , 2017 . 6 e16247 DOI:10.1038/lsa.2016.247http://doi.org/10.1038/lsa.2016.247 .

I. Ivankovic , 等 . Volumetric optoacoustic tomography enables non-invasive in vivo characterization of impaired heart function in hypoxic conditions . Sci. Rep. , 2019 . 9 8369 DOI:10.1038/s41598-019-44818-8http://doi.org/10.1038/s41598-019-44818-8 .

H. C. A. Lin , 等 . Ultrafast volumetric optoacoustic imaging of whole isolated beating mouse heart . Sci. Rep. , 2018 . 8 14132 DOI:10.1038/s41598-018-32317-1http://doi.org/10.1038/s41598-018-32317-1 .

Ç. Özsoy , 等 . Ultrafast four-dimensional imaging of cardiac mechanical wave propagation with sparse optoacoustic sensing . Proc. Natl Acad. Sci. USA , 2021 . 118 e2103979118 DOI:10.1073/pnas.2103979118http://doi.org/10.1073/pnas.2103979118 .

B. Desjardins , E. A. Kazerooni . ECG-gated cardiac CT . Am. J. Roentgenol. , 2004 . 182 993 -1010 . DOI:10.2214/ajr.182.4.1820993http://doi.org/10.2214/ajr.182.4.1820993 .

M. S. Nacif , 等 . Cardiac magnetic resonance imaging and its electrocardiographs (ECG): tips and tricks . Int. J. Cardiovasc. Imaging , 2012 . 28 1465 -1475 . DOI:10.1007/s10554-011-9957-4http://doi.org/10.1007/s10554-011-9957-4 .

M. L. Lassen , J. Kwiecinski , P. J. Slomka . Gating approaches in cardiac PET imaging . Pet. Clin. , 2019 . 14 271 -279 . DOI:10.1016/j.cpet.2018.12.008http://doi.org/10.1016/j.cpet.2018.12.008 .

L. Lin , 等 . High-speed three-dimensional photoacoustic computed tomography for preclinical research and clinical translation . Nat. Commun. , 2021 . 12 882 DOI:10.1038/s41467-021-21232-1http://doi.org/10.1038/s41467-021-21232-1 .

S. L. Jacques . Optical properties of biological tissues: a review . Phys. Med. Biol. , 2013 . 58 R37 -R61 . DOI:10.1088/0031-9155/58/11/R37http://doi.org/10.1088/0031-9155/58/11/R37 .

A. N. Price , 等 . Cardiovascular magnetic resonance imaging in experimental models . Open Cardiovasc. Med. J. , 2010 . 4 278 -292 . DOI:10.2174/1874192401004010278http://doi.org/10.2174/1874192401004010278 .

T. Azar , J. Sharp , D. Lawson . Heart rates of male and female sprague-dawley and spontaneously hypertensive rats housed singly or in groups . J. Am. Assoc. Lab. Anim. Sci. , 2011 . 50 175 -184. .

L. Lin , 等 . In vivo photoacoustic tomography of myoglobin oxygen saturation . J. Biomed. Opt. , 2015 . 21 061002 DOI:10.1117/1.JBO.21.6.061002http://doi.org/10.1117/1.JBO.21.6.061002 .

M. E. Piché , A. Tchernof , J. P. Després . Obesity phenotypes, diabetes, and cardiovascular diseases . Circ. Res. , 2020 . 126 1477 -1500 . DOI:10.1161/CIRCRESAHA.120.316101http://doi.org/10.1161/CIRCRESAHA.120.316101 .

L. T. Clark . Cholesterol and heart disease: current concepts in pathogenesis and treatment . J. Natl Med. Assoc. , 1986 . 78 743 -751. .

M. A. Alpert , M. W. Hashimi . Obesity and the heart . Am. J. Med. Sci. , 1993 . 306 117 -123 . DOI:10.1097/00000441-199308000-00011http://doi.org/10.1097/00000441-199308000-00011 .

G. P. Aurigemma , G. De Simone , T. P. Fitzgibbons . Cardiac remodeling in obesity . Circ.: Cardiovasc. Imag. , 2013 . 6 142 -152 . DOI:10.1161/CIRCIMAGING.111.964627http://doi.org/10.1161/CIRCIMAGING.111.964627.

Pantaleone, C. Cancer Immunotherapy and Biological Cancer Treatments (ed. Arnouk, H. ) (IntechOpen, 2019).

J. Mayet , A. Hughes . Cardiac and vascular pathophysiology in hypertension . Heart , 2003 . 89 1104 -1109 . DOI:10.1136/heart.89.9.1104http://doi.org/10.1136/heart.89.9.1104 .

S. M. Artham , 等 . Obesity and hypertension, heart failure, and coronary heart disease-risk factor, paradox, and recommendations for weight loss . Ochsner J. , 2009 . 9 124 -132. .

U. B. Hendgen-Cotta , M. Kelm , T. Rassaf . Myoglobin functions in the heart . Free Radic. Biol. Med. , 2014 . 73 252 -259 . DOI:10.1016/j.freeradbiomed.2014.05.005http://doi.org/10.1016/j.freeradbiomed.2014.05.005 .

P. Hu , 等 . Spatiotemporal antialiasing in photoacoustic computed tomography . IEEE Trans. Med. Imaging , 2020 . 39 3535 -3547 . DOI:10.1109/TMI.2020.2998509http://doi.org/10.1109/TMI.2020.2998509 .

American National Standards Institute. American National Standard for the Safe Use of Lasers ANSI, z136. 131-2014 (Laser Institute of America, 2014).

L. Li , 等 . Single-impulse panoramic photoacoustic computed tomography of small-animal whole-body dynamics at high spatiotemporal resolution . Nat. Biomed. Eng. , 2017 . 1 0071 DOI:10.1038/s41551-017-0071http://doi.org/10.1038/s41551-017-0071 .

M. Maggioni , 等 . Nonlocal transform-domain filter for volumetric data denoising and reconstruction . IEEE Trans. Image Process. , 2013 . 22 119 -133 . DOI:10.1109/TIP.2012.2210725http://doi.org/10.1109/TIP.2012.2210725 .

Frangi, A. F. et al. Multiscale vessel enhancement filtering. Proceedings of the First International Conference on Medical Image Computing and Computer-Assisted Intervention. (pp. 130–137. (Springer, Cambridge, 1998).

S. Cho , 等 . 3D PHOVIS: 3D photoacoustic visualization studio . Photoacoustics , 2020 . 18 100168 DOI:10.1016/j.pacs.2020.100168http://doi.org/10.1016/j.pacs.2020.100168 .

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