Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence

Leanne Maurice; Alberto Bilenca

doi:10.1038/s41377-023-01085-7

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Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence

Original Articles

- Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence
- Light: Science & Applications Vol. 12, Issue 3, Pages: 415-424(2023)
- Affiliations：
  
  1.Biomedical Engineering Department, Ben-Gurion University of the Negev, 1 Ben Gurion Blvd, Be'er-Sheva, 84105, Israel
  2.Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, 1 Ben Gurion Blvd, Be'er-Sheva, 84105, Israel
- Author bio：
  
  Alberto Bilenca (bilenca@bgu.ac.il)
- Funds：
- DOI：10.1038/s41377-023-01085-7
  CLC：
- Published：31 March 2023，
  
  Published Online：3 March 2023，
  
  Received：1 May 2022，
  
  Revised：1 February 2023，
  
  Accepted：1 February 2023
Scan for full text
Maurice, L. & Bilenca, A. Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence. Light: Science & Applications, 12, 415-424 (2023).
DOI：

Maurice, L. & Bilenca, A. Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence. Light: Science & Applications, 12, 415-424 (2023). DOI： 10.1038/s41377-023-01085-7.

摘要

Abstract

Single particle tracking in three dimensions is an indispensable tool for studying dynamic processes in various disciplines

including material sciences

physics

and biology

but often shows anisotropic three-dimensional spatial localization precision

which restricts the tracking precision

and/or a limited number of particles that can be tracked simultaneously over extended volumes. Here we developed an interferometric

three-dimensional fluorescence single particle tracking method based on conventional widefield excitation and temporal phase-shift interference of the emitted

high-aperture-angle

fluorescence wavefronts in a greatly simplified

free-running

triangle interferometer that enables tracking of multiple particles at the same time with

10-nm spatial localization precision in all three dimensions over extended volumes (~35 × 35 × 2 μm

) at video rate (25 Hz). We applied our method to characterize the microenvironment of living cells and up to ~40 μm deep in soft materials.

关键词

Keywords

references

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