Upconversion nanoscopy revealing surface heterogeneity of tumor-secreted extracellular vesicles

Rui Pu; Qiuqiang Zhan

doi:10.1038/s41377-023-01106-5

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Upconversion nanoscopy revealing surface heterogeneity of tumor-secreted extracellular vesicles

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- Upconversion nanoscopy revealing surface heterogeneity of tumor-secreted extracellular vesicles
- Light: Science & Applications Vol. 12, Issue 4, Pages: 502-504(2023)
- Affiliations：
  
  Centre for Optical and Electromagnetic Research, South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510006, China
- Author bio：
  
  Qiuqiang Zhan (zhanqiuqiang@m.scnu.edu.cn)
- Funds：
- DOI：10.1038/s41377-023-01106-5
  CLC：
- Published：30 April 2023，
  
  Published Online：3 March 2023，
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Pu, R. & Zhan, Q. Q. Upconversion nanoscopy revealing surface heterogeneity of tumor-secreted extracellular vesicles.Light: Science & Applications,12, 502-504 (2023).
DOI：

Pu, R. & Zhan, Q. Q. Upconversion nanoscopy revealing surface heterogeneity of tumor-secreted extracellular vesicles.Light: Science & Applications,12, 502-504 (2023). DOI： 10.1038/s41377-023-01106-5.

摘要

Abstract

Super-resolution microscopic imaging employing upconversion nanoparticles is applied to reveal the surface heterogeneity of tumor cell-derived small extracellular vesicles

i.e.

exosome. The number of surface antigens of every extracellular vesicles can be quantified by both the high imaging resolution and stable brightness of upconversion nanoparticles. This method proves its great potential in nanoscale biological studies.

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references

El Andaloussi, S. et al. Extracellular vesicles: biology and emerging therapeutic opportunities.Nat. Rev. Drug Discov.12, 347–357 (2013).

Huang, G. et al. Upconversion nanoparticles for super-resolution quantification of single small extracellular vesicles.eLight2, 20 (2022).

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Chen, C. H. et al. Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles.Nat. Commun.9, 3290 (2018).

Liang, Y. S. et al. Migrating photon avalanche in different emitters at the nanoscale enables 46th-order optical nonlinearity.Nat. Nanotechnol.17, 524–530 (2022).

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Zhan, Q. Q. et al. Achieving high-efficiency emission depletion nanoscopy by employing cross relaxation in upconversion nanoparticles.Nat. Commun.8, 1058 (2017).

Pu, R. et al. Super-resolution microscopy enabled by high-efficiency surface-migration emission depletion.Nat. Commun.13, 6636 (2022).

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