A high-precision multi-dimensional microspectroscopic technique for morphological and properties analysis of cancer cell

Lirong Qiu; Yunhao Su; Ke-Mi Xu; Han Cui; Dezhi Zheng; Yuanmin Zhu; Lin Li; Fang Li; Weiqian Zhao

doi:10.1038/s41377-023-01153-y

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A high-precision multi-dimensional microspectroscopic technique for morphological and properties analysis of cancer cell

Original Articles

- A high-precision multi-dimensional microspectroscopic technique for morphological and properties analysis of cancer cell
- Light: Science & Applications Vol. 12, Issue 6, Pages: 1117-1126(2023)
- Affiliations：
  
  1.MIIT Key Laboratory of Complex-field Intelligent Exploration, School of Optics and Photonics, Beijing Institute of Technology, 100081 Beijing, China
  2.Department of Gastroenterology, Aerospace Central Hospital, Peking University Aerospace School of Clinical Medicine, 100081 Beijing, China
  3.Department of Pathology, Aerospace Central Hospital, Peking University Aerospace School of Clinical Medicine, 100081 Beijing, China
- Author bio：
  
  Weiqian Zhao (zwq669@126.com)
- Funds：
- DOI：10.1038/s41377-023-01153-y
  CLC：
- Published：30 June 2023，
  
  Published Online：29 May 2023，
  
  Received：11 October 2022，
  
  Revised：19 March 2023，
  
  Accepted：11 April 2023
Scan for full text
Qiu, L. R. et al. A high-precision multi-dimensional microspectroscopic technique for morphological and properties analysis of cancer cell. Light: Science & Applications, 12, 1117-1126 (2023).
DOI：

Qiu, L. R. et al. A high-precision multi-dimensional microspectroscopic technique for morphological and properties analysis of cancer cell. Light: Science & Applications, 12, 1117-1126 (2023). DOI： 10.1038/s41377-023-01153-y.

摘要

Abstract

Raman and Brillouin scattering are sensitive approaches to detect chemical composition and mechanical elasticity pathology of cells in cancer development and their medical treatment researches. The application is

however

suffering from the lack of ability to synchronously acquire the scattering signals following three-dimensional (3D) cell morphology with reasonable spatial resolution and signal-to-noise ratio. Herein

we propose a divided-aperture laser differential confocal 3D Geometry-Raman-Brillouin microscopic detection technology

by which reflection

Raman

and Brillouin scattering signals are simultaneously in situ collected in real time with an axial focusing accuracy up to 1 nm

in the height range of 200 μm. The divided aperture improves the anti-noise capability of the system

and the noise influence depth of Raman detection reduces by 35.4%

and the Brillouin extinction ratio increases by 22 dB. A high-precision multichannel microspectroscopic system containing these functions is developed

which is utilized to study gastric cancer tissue. As a result

a 25% reduction of collagen concentration

42% increase of DNA substances

17% and 9% decrease in viscosity and elasticity are finely resolved from the 3D mappings. These findings indicate that our system can be a powerful tool to study cancer development new therapies at the sub-cell level.

关键词

Keywords

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