Single probes and resonant four-wave-mixing enabling novel correlative light electron microscopy workflow

Long Chen; Chao He

doi:10.1038/s41377-023-01154-x

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Single probes and resonant four-wave-mixing enabling novel correlative light electron microscopy workflow

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- Single probes and resonant four-wave-mixing enabling novel correlative light electron microscopy workflow
- Light: Science & Applications Vol. 12, Issue 6, Pages: 933-934(2023)
- Affiliations：
  
  1.Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK
  2.Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK
- Author bio：
  
  Long Chen (long.chen@strubi.ox.ac.uk)
  Chao He (chao.he@eng.ox.ac.uk)
- Funds：
- DOI：10.1038/s41377-023-01154-x
  CLC：
- Published：30 June 2023，
  
  Published Online：8 May 2023，
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Chen, L. & He, C. Single probes and resonant four-wave-mixing enabling novel correlative light electron microscopy workflow. Light: Science & Applications, 12, 933-934 (2023).
DOI：

Chen, L. & He, C. Single probes and resonant four-wave-mixing enabling novel correlative light electron microscopy workflow. Light: Science & Applications, 12, 933-934 (2023). DOI： 10.1038/s41377-023-01154-x.

摘要

Abstract

Correlative light electron microscopy prefers single probes with stable performance in both optical and electron microscopy. Now researchers have shown how to harness gold nanoparticles featuring exceptional photostability and four-wave-mixing nonlinearity to realize a new correlation imaging approach.

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references

vandenDries,K.,Fransen,J.&Cambi,A.FluorescenceCLEMinbiology:historicdevelopmentsandcurrentsuper-resolutionapplications.FEBS Lett.596,2486–2496(2022)..

Begemann,I.&Galic,M.Correlativelightelectronmicroscopy:connectingsynapticstructureandfunction.Front. Synaptic Neurosci.8,28(2016)..

Kukulski,W.etal.Correlatedfluorescenceand3Delectronmicroscopywithhighsensitivityandspatialprecision.J. Cell Biol.192,111–119(2011)..

Giepmans,B.N.G.etal.CorrelatedlightandelectronmicroscopicimagingofmultipleendogenousproteinsusingQuantumdots.Nat. Methods2,743–749(2005)..

Tanner,H.etal.Fluorescentplatinumnanoclustersascorrelativelightelectronmicroscopyprobes.Methods Cell Biol.162,39–68(2021)..

Zoriniants,G.etal.Background-free3Dnanometriclocalizationandsub-nmasymmetrydetectionofsingleplasmonicnanoparticlesbyfour-wavemixinginterferometrywithopticalvortices.Phys. Rev. X7,041022(2017)..

Pope,I.etal.Correlativelight-electronmicroscopyusingsmallgoldnanoparticlesassingleprobes.Light Sci. Appl.12,80,https://doi.org/10.1038/s41377-023-01115-4(2023)..

Liu,S.L.etal.Single-virustracking:fromimagingmethodologiestovirologicalapplications.Chem. Rev.120,1936–1979(2020)..

Jiang,Z.D.etal.GeneticallyencodedtagsfordirectsynthesisofEM-visiblegoldnanoparticlesincells.Nat. Methods17,937–946(2020)..

Payne,L.M.etal.Theopticalnanosizer-quantitativesizeandshapeanalysisofindividualnanoparticlesbyhigh-throughputwidefieldextinctionmicroscopy.Nanoscale12,16215–16228(2020)..

Wu,P.H.etal.High-throughputballisticinjectionnanorheologytomeasurecellmechanics.Nat. Protoc.7,155–170(2012)..

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