CRISPR-powered optothermal nanotweezers: Diverse bio-nanoparticle manipulation and single nucleotide identification

Jiajie Chen; Zhi Chen; Changle Meng; Jianxing Zhou; Yuhang Peng; Xiaoqi Dai; Jingfeng Li; Yili Zhong; Xiaolin Chen; Wu Yuan; Ho-Pui Ho; Bruce Zhi Gao; Junle Qu; Xueji Zhang; Han Zhang; Yonghong Shao

doi:10.1038/s41377-023-01326-9

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CRISPR-powered optothermal nanotweezers: Diverse bio-nanoparticle manipulation and single nucleotide identification

Original Articles

- CRISPR-powered optothermal nanotweezers: Diverse bio-nanoparticle manipulation and single nucleotide identification
- Light: Science & Applications Vol. 12, Issue 12, Pages: 2644-2654(2023)
- Affiliations：
  
  1.State Key Laboratory of Radio Frequency Heterogeneous Integration, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen 518060, China
  2.Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong, China
  3.Department of Bioengineering and COMSET, Clemson University, Clemson, SC 29634, USA
  4.School of Biomedical Engineering, Shenzhen University, Shenzhen, Guangdong 518060, China
- Author bio：
  
  Jiajie Chen (cjj@szu.edu.cn)
  Ho-Pui Ho (aaron.ho@cuhk.edu.hk)
  Han Zhang (hzhang@szu.edu.cn)
  Yonghong Shao (shaoyh@szu.edu.cn)
- Funds：
- DOI：10.1038/s41377-023-01326-9
  CLC：
- Published：31 December 2023，
  
  Published Online：16 November 2023，
  
  Received：26 June 2023，
  
  Revised：26 October 2023，
  
  Accepted：03 November 2023
- Accepted：
Scan QR Code
Chen, J. J. et al. CRISPR-powered optothermal nanotweezers: Diverse bio-nanoparticle manipulation and single nucleotide identification. Light: Science & Applications, 12, 2644-2654 (2023).
DOI：

Chen, J. J. et al. CRISPR-powered optothermal nanotweezers: Diverse bio-nanoparticle manipulation and single nucleotide identification. Light: Science & Applications, 12, 2644-2654 (2023). DOI： 10.1038/s41377-023-01326-9.

摘要

Abstract

Optothermal nanotweezers have emerged as an innovative optical manipulation technique in the past decade

which revolutionized classical optical manipulation by efficiently capturing a broader range of nanoparticles. However

the optothermal temperature field was merely employed for in-situ manipulation of nanoparticles

its potential for identifying bio-nanoparticles remains largely untapped. Hence

based on the synergistic effect of optothermal manipulation and CRIPSR-based bio-detection

we developed CRISPR-powered optothermal nanotweezers (CRONT). Specifically

by harnessing diffusiophoresis and thermo-osmotic flows near the substrate upon optothermal excitation

we successfully trapped and enriched DNA functionalized gold nanoparticles

CRISPR-associated proteins

as well as DNA strands. Remarkably

we built an optothermal scheme for enhancing CRISPR-based single-nucleotide polymorphism (SNP) detection at single molecule level

while also introducing a novel CRISPR methodology for observing nucleotide cleavage. Therefore

this innovative approach has endowed optical tweezers with DNA identification ability in aqueous solution which was unattainable before. With its high specificity and feasibility for in-situ bio-nanoparticle manipulation and identification

CRONT will become a universal tool in point-of-care diagnosis

biophotonics

and bio-nanotechnology.

关键词

Keywords

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