High-resolution multimodal flexible coherent Raman endoscope

Alberto Lombardini; Vasyl Mytskaniuk; Siddharth Sivankutty; Esben Ravn Andresen; Xueqin Chen; Jérôme Wenger; Marc Fabert; Nicolas Joly; Frédéric Louradour; Alexandre Kudlinski; Hervé Rigneault

doi:10.1038/s41377-018-0003-3

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High-resolution multimodal flexible coherent Raman endoscope

Original Articles

- High-resolution multimodal flexible coherent Raman endoscope
- High-resolution multimodal flexible coherent Raman endoscope
- LSA 2018年7卷第4期页码：87-94
- Affiliations：
  
  1.Aix-Marseille Univ, CNRS, Centrale Marseille, Institut Fresnel, Marseille, France
  2.Laboratoire de Physique des Lasers Atomes et Molécules, UMR 8523, CNRS, Université Lille, 59000 Lille, France
  3.CNRS, XLIM, UMR 7252, Université de Limoges, 87060 Limoges, France
  4.Department of Physics, Max Planck Institute for the Science of Light, University of Erlangen Nuremberg, 91058 Erlangen, Germany
- Author bio：
  
  Hervé. Rigneault (herve.rigneault@fresnel.fr)
- Funds：
- DOI：10.1038/s41377-018-0003-3
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2018-05-30，
  
  收稿日期：2017-09-21，
  
  修回日期：2018-02-12，
  
  录用日期：2018-02-12
- Accepted：
Scan QR Code
High-resolution multimodal flexible coherent Raman endoscope[J]. LSA, 2018,7(4):87-94.

Lombardini, A. et al. High-resolution multimodal flexible coherent Raman endoscope. Light: Science & Applications, 5, 87-94 (2018).
High-resolution multimodal flexible coherent Raman endoscope[J]. LSA, 2018,7(4):87-94. DOI： 10.1038/s41377-018-0003-3.

Lombardini, A. et al. High-resolution multimodal flexible coherent Raman endoscope. Light: Science & Applications, 5, 87-94 (2018). DOI： 10.1038/s41377-018-0003-3.

摘要

Abstract

Coherent Raman scattering microscopy is a fast

label-free

and chemically specific imaging technique that shows high potential for future in vivo optical histology. However

the imaging depth in tissues is limited to the sub-millimeter range because of absorption and scattering. Realization of coherent Raman imaging using a fiber endoscope system is a crucial step towards imaging deep inside living tissues and providing information that is inaccessible with current microscopy tools. Until now

the development of coherent Raman endoscopy has been hampered by several issues

mainly related to the fiber delivery of the excitation pulses and signal collection. Here

we present a flexible

compact

coherent Raman

and multimodal nonlinear endoscope (4.2 mm outer diameter

71 mm rigid length) based on a resonantly scanned hollow-core Kagomé-lattice double-clad fiber. The fiber design enables distortion-less

background-free delivery of femtosecond excitation pulses and back-collection of nonlinear signals through the same fiber. Sub-micrometer spatial resolution over a large field of view is obtained by combination of a miniature objective lens with a silica microsphere lens inserted into the fiber core. We demonstrate high-resolution

high-contrast coherent anti-Stokes Raman scattering

and second harmonic generation endoscopic imaging of biological tissues over a field of view of 320 µm at a rate of 0.8 frames per second. These results pave the way for intraoperative label-free imaging applied to real-time histopathology diagnosis and surgery guidance.

关键词

Keywords

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