Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach

Dominik Schneidereit; Stefanie Nübler; Gerhard Prölß; Barbara Reischl; Sebastian Schürmann; Oliver J Müller; Oliver Friedrich

doi:10.1038/s41377-018-0080-3

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Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach

Original Articles

- Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach
- Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach
- 光：科学与应用 2018年7卷第6期页码：797-810
- Affiliations：
  
  1.Institute of Medical Biotechnology, Friedrich-Alexander-University (FAU) Erlangen-Nürnberg, Paul-Gordan-Str. 3, 91052, Erlangen, Germany
  2.Erlangen Graduate School in Advanced Optical Technologies (SAOT), FAU Erlangen-Nürnberg, Paul-Gordan-Str. 7, 91052, Erlangen, Germany
  3.Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany
  4.Department of Internal Medicine Ⅲ, University of Kiel, Arnold-Heller-Str. 3, 24105, Kiel, Germany
  5.DZHK (German Center for Cardiovascular Research) Partner Site Hamburg/Kiel/Lübeck, Kiel, Germany
- Author bio：
  
  Oliver Friedrich (oliver.friedrich@mbt.uni-erlangen.de)
- Funds：
- DOI：10.1038/s41377-018-0080-3
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Dominik Schneidereit, Stefanie Nübler, Gerhard Prölß, 等. Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach[J]. 光：科学与应用, 2018,7(6):797-810.

Schneidereit, D. et al. Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach. Light: Science & Applications, 6, 797-810 (2018).
Dominik Schneidereit, Stefanie Nübler, Gerhard Prölß, 等. Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach[J]. 光：科学与应用, 2018,7(6):797-810. DOI： 10.1038/s41377-018-0080-3.

Schneidereit, D. et al. Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach. Light: Science & Applications, 6, 797-810 (2018). DOI： 10.1038/s41377-018-0080-3.

摘要

Abstract

Skeletal muscle is an archetypal organ whose structure is tuned to match function. The magnitude of order in muscle fibers and myofibrils containing motor protein polymers determines the directed force output of the summed force vectors and, therefore, the muscle's power performance on the structural level. Structure and function can change dramatically during disease states involving chronic remodeling. Cellular remodeling of the cytoarchitecture has been pursued using noninvasive and label-free multiphoton second harmonic generation (SHG) microscopy. Hereby, structure parameters can be extracted as a measure of myofibrillar order and thus are suggestive of the force output that a remodeled structure can still achieve. However, to date, the parameters have only been an indirect measure, and a precise calibration of optical SHG assessment for an exerted force has been elusive as no technology in existence correlates these factors. We engineered a novel, automated, high-precision biomechatronics system into a multiphoton microscope allows simultaneous isometric Ca,2+,-graded force or passive viscoelasticity measurements and SHG recordings. Using this ,MechaMorph, system, we studied force and SHG in single EDL muscle fibers from wt and ,mdx, mice; the latter serves as a model for compromised force and abnormal myofibrillar structure. We present Ca,2+,-graded isometric force, pCa-force curves, passive viscoelastic parameters and 3D structure in the same fiber for the first time. Furthermore, we provide a direct calibration of isometric force to morphology, which allows noninvasive prediction of the force output of single fibers from only multiphoton images, suggesting a potential application in the diagnosis of myopathies.

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references

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