High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization

Wei Zhang; Yanxiu Li; Van Phuc Nguyen; Ziyi Huang; Zhipeng Liu; Xueding Wang; Yannis M. Paulus

doi:10.1038/s41377-018-0093-y

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High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization

Original Articles

- High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization
- High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization
- 光：科学与应用 2018年7卷第6期页码：956-967
- Affiliations：
  
  1.Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48105, USA
  2.Institution of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, China
  3.Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI, 48105, USA
  4.Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China
  5.Department of Radiology, University of Michigan, Ann Arbor, MI, 48105, USA
- Author bio：
  
  Zhipeng Liu (lzpeng67@163.com)
  Zhipeng Liu (lzpeng67@163.com)
  Zhipeng Liu (lzpeng67@163.com)
- Funds：
- DOI：10.1038/s41377-018-0093-y
  中图分类号：
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Wei Zhang, Yanxiu Li, Van Phuc Nguyen, 等. High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization[J]. 光：科学与应用, 2018,7(6):956-967.

Zhang, W. et al. High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization. Light: Science & Applications, 7, 956-967 (2018).
Wei Zhang, Yanxiu Li, Van Phuc Nguyen, 等. High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization[J]. 光：科学与应用, 2018,7(6):956-967. DOI： 10.1038/s41377-018-0093-y.

Zhang, W. et al. High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization. Light: Science & Applications, 7, 956-967 (2018). DOI： 10.1038/s41377-018-0093-y.

摘要

Abstract

Photoacoustic microscopy (PAM) is an emerging imaging technology that can non-invasively visualize ocular structures in animal eyes. This report describes an integrated multimodality imaging system that combines PAM, optical coherence tomography (OCT), and fluorescence microscopy (FM) to evaluate angiogenesis in larger animal eyes. High-resolution in vivo imaging was performed in live rabbit eyes with vascular endothelial growth factor (VEGF)-induced retinal neovascularization (RNV). The results demonstrate that our multimodality imaging system can non-invasively visualize RNV in both albino and pigmented rabbits to determine retinal pathology using PAM and OCT and verify the leakage of neovascularization using FM and fluorescein dye. This work presents high-resolution visualization of angiogenesis in rabbits using a multimodality PAM, OCT, and FM system and may represent a major step toward the clinical translation of the technology.

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references

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