High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging

Sergey Turtaev; Ivo T. Leite; Tristan Altwegg-Boussac; Janelle M. P. Pakan; Nathalie L. Rochefort; Tomáš Čižmár

doi:10.1038/s41377-018-0094-x

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High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging

Letter

- High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging
- High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging
- 光：科学与应用 2018年7卷第6期页码：663-670
- Affiliations：
  
  1.Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, Jena, 07745, Germany
  2.School of Life Sciences, University of Dundee, Nethergate, Dundee, DD1 4HN, UK
  3.School of Science and Engineering, University of Dundee, Nethergate, Dundee, DD1 4HN, UK
  4.Centre for Discovery Brain Sciences, University of Edinburgh, Hugh Robson Building 15, George Square, Edinburgh, EH8 9XD, UK
  5.Center for Behavioral Brain Sciences, Institute of Cognitive Neurology and Dementia Research, German Center for Neurodegenerative Diseases, Leipziger Straße 44, Haus 64, Magdeburg, 39120, Germany
  6.Simons Initiative for the Developing Brain, University of Edinburgh, Edinburgh, EH8 9XD, UK
  7.Institute of Scientific Instruments of CAS, Kralovopolska 147, Brno, 612 64, Czech Republic
- Author bio：
  
  Nathalie L. Rochefort (n.rochefort@ed.ac.uk)
  Tomáš. Čižmár (Tomas.Cizmar@leibniz-ipht.de)
- Funds：
- DOI：10.1038/s41377-018-0094-x
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Sergey Turtaev, Ivo T. Leite, Tristan Altwegg-Boussac, 等. High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging[J]. 光：科学与应用, 2018,7(6):663-670.

Turtaev, S. et al. High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging. Light: Science & Applications, 7, 663-670 (2018).
Sergey Turtaev, Ivo T. Leite, Tristan Altwegg-Boussac, 等. High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging[J]. 光：科学与应用, 2018,7(6):663-670. DOI： 10.1038/s41377-018-0094-x.

Turtaev, S. et al. High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging. Light: Science & Applications, 7, 663-670 (2018). DOI： 10.1038/s41377-018-0094-x.

摘要

Abstract

Progress in neuroscience relies on new techniques for investigating the complex dynamics of neuronal networks. An ongoing challenge is to achieve minimally invasive and high-resolution observations of neuronal activity in vivo inside deep brain areas. Recently introduced methods for holographic control of light propagation in complex media enable the use of a hair-thin multimode optical fibre as an ultranarrow imaging tool. Compared to endoscopes based on graded-index lenses or fibre bundles, this new approach offers a footprint reduction exceeding an order of magnitude, combined with a significant enhancement in resolution. We designed a compact and high-speed system for fluorescent imaging at the tip of a fibre, achieving a resolution of 1.18 ± 0.04 µm across a 50-µm field of view, yielding 7-kilopixel images at a rate of 3.5 frames/s. Furthermore, we demonstrate in vivo observations of cell bodies and processes of inhibitory neurons within deep layers of the visual cortex and hippocampus of anaesthetised mice. This study paves the way for modern microscopy to be applied deep inside tissues of living animal models while exerting a minimal impact on their structural and functional properties.

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references

Mosk, A. P., Lagendijk, A., Lerosey, G. & Fink, M. Controlling waves in space and time for imaging and focusing in complex media. Nat. Photonics 6, 283–292 (2012).

Di Leonardo, R. & Bianchi, S. Hologram transmission through multi-mode optical fibers. Opt. Express 19, 247–254 (2011).

Čižmár, T. & Dholakia, K. Shaping the light transmission through a multimode optical fibre: complex transformation analysis and applications in biophotonics. Opt. Express 19, 18871–18884 (2011).

Čižmár, T. & Dholakia, K. Exploiting multimode waveguides for pure fibre-based imaging. Nat. Commun. 3, 1027 (2012).

Papadopoulos, I. N., Farahi, S., Moser, C. & Psaltis, D. Focusing and scanning light through a multimode optical fiber using digital phase conjugation. Opt. Express 20, 10583–10590 (2012).

Choi, Y. et al. Scanner-free and wide-field endoscopic imaging by using a single multimode optical fiber. Phys. Rev. Lett. 109, 203901 (2012).

Papadopoulos, I. N., Farahi, S., Moser, C. & Psaltis, D. High-resolution, lensless endoscope based on digital scanning through a multimode optical fiber. Biomed. Opt. Express 4, 260–270 (2013).

Plöschner, M., Tyc, T. & Čižmár, T. Seeing through chaos in multimode fibres. Nat. Photonics 9, 529–535 (2015).

Morales-Delgado, E. E., Psaltis, D. & Moser, C. Two-photon imaging through a multimode fiber. Opt. Express 23, 32158–32170 (2015).

Plöschner, M. et al. Multimode fibre: Light-sheet microscopy at the tip of a needle. Sci. Rep. 5, 18050 (2015).

Leite, I. T. et al. Three-dimensional holographic optical manipulation through a high-numerical-aperture soft-glass multimode fibre. Nat. Photonics 12, 33–39 (2018).

Vellekoop, I. M. & Mosk, A. P. Focusing coherent light through opaque strongly scattering media. Opt. Lett. 32, 2309–2311 (2007).

Vellekoop, I. M. & Mosk, A. P. Universal optimal transmission of light through disordered materials. Phys. Rev. Lett. 101, 120601 (2008).

Popoff, S., Lerosey, G., Fink, M., Boccara, A. C. & Gigan, S. Image transmission through an opaque material. Nat. Commun. 1, 81 (2010).

Popoff, S. M. et al. Measuring the transmission matrix in optics: an approach to the study and control of light propagation in disordered media. Phys. Rev. Lett. 104, 100601 (2010).

Conkey, D. B., Caravaca-Aguirre, A. M. & Piestun, R. High-speed scattering medium characterization with application to focusing light through turbid media. Opt. Express 20, 1733–1740 (2012).

Goorden, S. A., Bertolotti, J. & Mosk, A. P. Superpixel-based spatial amplitude and phase modulation using a digital micromirror device. Opt. Express 22, 17999–18009 (2014).

Akbulut, D., Huisman, T. J., Van Putten, E. G., Vos, W. L. & Mosk, A. P. Focusing light through random photonic media by binary amplitude modulation.Opt. Express 19, 4017–4029 (2011).

Caravaca-Aguirre, A. M., Niv, E., Conkey, D. B. & Piestun, R. Real-time resilient focusing through a bending multimode fiber. Opt. Express 21, 12881–12887 (2013).

Turtaev, S. et al. Comparison of nematic liquid-crystal and DMD based spatial light modulation in complex photonics. Opt. Express 25, 29874–29884 (2017).

Ohayon, S., Caravaca-Aguirre, A., Piestun, R. & DiCarlo, J. J. Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging. Biomed. Opt. Express 9, 1492–1509 (2018).

Kim, C. K., Adhikari, A. & Deisseroth, K. Integration of optogenetics with complementary methodologies in systems neuroscience. Nat. Rev. Neurosci. 18, 222–235 (2017).

Sharp, A. A., Ortega, A. M., Restrepo, D., Curran-Everett, D. & Gall, K. I n vivo penetration mechanics and mechanical properties of mouse brain tissue at micrometer scales.Ieee. Trans. Biomed. Eng. 56, 45–53 (2009).

Kozai, T. D. Y., Jaquins-Gerstl, A. S., Vazquez, A. L., Michael, A. C. & Cui, X. T. Brain tissue responses to neural implants impact signal sensitivity and intervention strategies. ACS Chem. Neurosci. 6, 48–67 (2015).

Chandrasekaran S. N., Ligtenberg H., Steenbergen W., Vellekoop I. M. Using digital micromirror devices for focusing light through turbid media. In Proc. Emerging Digital Micromirror Device Based Systems and Applications VI 897905(SPIE: San Francisco, California, USA, 2014).

Attardo, A., Fitzgerald, J. E. & Schnitzer, M. J. Impermanence of dendritic spines in live adult CA1 hippocampus. Nature 523, 592–596 (2015).

Sato, M., Kawano, M., Yanagawa, Y. & Hayashi, Y. I n vivo two-photon imaging of striatal neuronal circuits in mice. Neurobiol. Learn. Mem. 135, 146–151 (2016).

Lee, W. H. Binary computer-generated holograms. Appl. Opt. 18, 3661–3669 (1979).

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