Sharpening emitter localization in front of a tuned mirror

Hannah S. Heil; Benjamin Schreiber; Ralph Götz; Monika Emmerling; Marie-Christine Dabauvalle; Georg Krohne; Sven Höfling; Martin Kamp; Markus Sauer; Katrin G. Heinze

doi:10.1038/s41377-018-0104-z

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Sharpening emitter localization in front of a tuned mirror

Letter

- Sharpening emitter localization in front of a tuned mirror
- Sharpening emitter localization in front of a tuned mirror
- 光：科学与应用 2018年7卷第6期页码：671-678
- Affiliations：
  
  1.Rudolf Virchow Center, Research Center for Experimental Biomedicine, University of Würzburg, Josef-Schneider-Str.2, 97080, Würzburg, Germany
  2.Department of Biotechnology and Biophysics, Biozentrum, University of Würzburg, Am Hubland, 97074, Würzburg, Germany
  3.Technische Physik, Physikalisches Institut and Wilhelm Conrad Röntgen-Center for Complex Material Systems, University of Würzburg, Am Hubland, 97074, Würzburg, Germany
  4.Division of Electron Microscopy, Biozentrum, University of Würzburg, Am Hubland, 97074, Würzburg, Germany
  5.SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, UK
- Author bio：
  
  Markus Sauer (m.sauer@uni-wuerzburg.de)
  Katrin G. Heinze (katrin.heinze@virchow.uni-wuerzburg.de)
- Funds：
- DOI：10.1038/s41377-018-0104-z
  中图分类号：
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Hannah S. Heil, Benjamin Schreiber, Ralph Götz, 等. Sharpening emitter localization in front of a tuned mirror[J]. 光：科学与应用, 2018,7(6):671-678.

Heil, H. S. et al. Sharpening emitter localization in front of a tuned mirror. Light: Science & Applications, 7, 671-678 (2018).
Hannah S. Heil, Benjamin Schreiber, Ralph Götz, 等. Sharpening emitter localization in front of a tuned mirror[J]. 光：科学与应用, 2018,7(6):671-678. DOI： 10.1038/s41377-018-0104-z.

Heil, H. S. et al. Sharpening emitter localization in front of a tuned mirror. Light: Science & Applications, 7, 671-678 (2018). DOI： 10.1038/s41377-018-0104-z.

摘要

Abstract

Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio,1,1,. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror,2,2,-,4,4,2-4,., Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.

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references

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